1887

Abstract

SUMMARY: Indicator plates containing eosin, methylene blue, glucosamine and proline were used to select mutants of impaired in the utilization of glucosamine. One such mutant, strain hOG298, grew on glucosamine at a slower rate than the parent and was severely impaired in growth on -acetylglucosamine. The mutant was unable to express the first three steps in the -acetylglucosamine pathway: the permease, -acetylglucosamine kinase and -acetylglucos-amine-6-phosphate deacetylase. Glucosamine-6-phosphate deaminase was, however, induced by -acetylglucosamine. The mutant still possessed a constitutive uptake system and kinase activity for glucosamine but glucosamine neither increased the glucosamine kinase activity nor induced -acetylglucosamine kinase. These findings accounted for the decreased growth rate on glucosamine. The parent strain formed germ-tubes in -acetylglucosamine or 4% (v/v) serum but the mutant formed germ-tubes only in serum.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-1-15
1986-01-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/1/mic-132-1-15.html?itemId=/content/journal/micro/10.1099/00221287-132-1-15&mimeType=html&fmt=ahah

References

  1. Dabrowa N., Howard D. H. 1983; Blastoconidium germination. In Fungi Pathogenic for Humans and Animals, pp. 525–545 Edited by D. H. Howard. New York & Basel: Marcel Dekker.
    [Google Scholar]
  2. Eggstein M., Kreutz F. H. 1967; Techniques in Protein Chemistry, p. 340–340 Edited by J. L. Bailey. London: Elsevier.
    [Google Scholar]
  3. Gopal P. K., Sullivan P. A., Shepherd M. G. 1982; Enzymes of N-acetylglucosamine metabolism during germ-tube formation in Candida albicans. Journal of General Microbiology 128:2319–2326
    [Google Scholar]
  4. Mattia E., Cassone A. 1979; Inducibility of germ-tube formation in Candida albicans at different phases of yeast growth. Journal of General Microbiology 113:439–442
    [Google Scholar]
  5. Mattia E., Carruba G., Angiolella L., Cassone A. 1982; Induction of germ tube formation by N-acetyl-d-glucosamine in Candida albicans: uptake of inducer and germinative response. Journal of Bacteriology 152:555–562
    [Google Scholar]
  6. Odds F. C. 1979; Data chart for yeast pathogens. In Candida and Candidosis, pp. 253–257 Leicester: Leicester University Press.
    [Google Scholar]
  7. Poulter R., Jeffery K., Hubbard M. J., Shepherd M. G., Sullivan P. A. 1981; Parasexual genetic analysis of Candida albicans by spheroplast fusion. Journal of Bacteriology 146:833–840
    [Google Scholar]
  8. Shepherd M. G., Sullivan P. A. 1976; The production and growth characteristics of yeast and mycelial forms of Candida albicans in continuous culture. Journal of General Microbiology 93:361–370
    [Google Scholar]
  9. Shepherd M. G., Sullivan P. A. 1983; Candida albicans germ-tube formation with immobilized GlcNAc. FEMS Microbiology Letters 17:167–170
    [Google Scholar]
  10. Shepherd M. G., Sullivan P. A. 1984; The control of morphogenesis in Candida albicans. Journal of Dental Research 63:435–440
    [Google Scholar]
  11. Shepherd M. G., Chiew Y. Y., Ram S. P., Sullivan P. A. 1980a; Germ-tube induction in Candida albicans. Canadian Journal of Microbiology 26:21–26
    [Google Scholar]
  12. Shepherd M. G., Ghazali H. M., Sullivan P. A. 1980b; N-Acetyl-d-glucosamine kinase and germ-tube formation in Candida albicans. Experimental Mycology 4:147–159
    [Google Scholar]
  13. Simonetti N., Strippoli V., Cassone A. 1974; Yeast–mycelial conversion induced by N-acetyl-d-glucosamine in Candida albicans. Nature, London 250:344–346
    [Google Scholar]
  14. Singh B. R., Datta A. 1979; Induction of N-acetylglucosamine-catabolic pathway in sphero-plasts of Candida albicans. Biochemical Journal 178:427–431
    [Google Scholar]
  15. Sullivan P. A., Shepherd M. G. 1982; Gratuitous induction by N-acetylmannosamine of germ-tube formation and enzymes for N-acetylglucos-amine utilisation in Candida albicans. Journal of Bacteriology 151:1118–1122
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-1-15
Loading
/content/journal/micro/10.1099/00221287-132-1-15
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error