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Volume 131, Issue 09

Dimorphism in : Contribution of Mannoproteins to the Architecture of Yeast and Mycelial Cell Walls Free

Abstract

Summary: Wall mannoproteins of the two (yeast and mycelial) cellular forms of were solubilized by different agents. Boiling in 2% (w/v) SDS was the best method, as more than70% of the total mannoprotein was extracted. Over 40 different bands (from 15 to 80 kDal) were detected on SDS-polyacrylamide gel electrophoresis of this material. The residual wall mannoproteins were releasedafter enzymic (Zymolyase and endogenous wall -glucanases) degradation of wall glucan, suggesting that theyare covalently linked to this structural polymer. Four bands (of 160 kDal, 205 kDal and higher molecular mass) were observed in the material released from yeast walls but only the two smaller components weredetected in the material obtained from mycelial walls. Moreover, the mannoproteins of high molecular mass, which are covalently linked in walls of normal cells, were not incorporated into walls of regeneratingprotoplasts, but non-covalently linked mannoproteins were retained from the beginning of the process.

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© Society for General Microbiology 1985
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1985-09-01
2025-03-22
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References

  1. Cassone A., Kerridge D., Gale E. F. 1979; Ultrastructural changes in the cell wall of Candida albicans following cessation of growth and their possible relationship to thedevelopment of polyene resistance. Journal of General Microbiology 110:339–349
     [Google Scholar]
  2. Chaffin w. L., Stocco D.M. 1983; Cell wall proteins of Candida albicans. Canadian Journal of Microbiology 29:1438–1444
     [Google Scholar]
  3. Chattaway F. W., Holmes M. R., Barlow J. E. 1968; Cell wall composition ofthe mycelial and blastospore forms of Candida albicans. Journal of General Microbiology 51:367–376
     [Google Scholar]
  4. Elorza M. V., Rico H., Gozalbo D., Sentandreu R., Dreu R. 1983; Cell wall composition and proto-plast regeneration in Candida albicans. Antonie van Leeuwenhoek 49:457–469
     [Google Scholar]
  5. Farks V., Svoboda A., Bauer S. 1970; Secretion of cell wall glycoprotein by yeast protoplasts. Effect of 2-deoxy-o-glucose and cycloheximide. Biochemical Journal 118:755–758
     [Google Scholar]
  6. Gopal P., Sullivan P. A., Shepherd M. G. 1984; Metabolism of [14C]glucose by regeneratingspheroplasts of Candida albicans. Journal of General Microbiology 130:325–335
     [Google Scholar]
  7. Herrero E., Pastor J., Sentandreu R. 1982; Turnover of protein components of the plasma membrane ofSaccharomyces cerevisiae. Biochimica et biophysica acta 689:38–44
     [Google Scholar]
  8. Kolarova N., Masler L., Sikl D. 1973; Cell wall glycopeptides of Candida albicansserotypes A and B. Biochimica et biophysica acta 328:221–227
     [Google Scholar]
  9. Lee K. L., Buckley H. R., Campbell C. 1975; An amino acid liquid synthetic medium for development of mycelial and yeast forms of Candida albicans. Sabouraudia 13:148–153
     [Google Scholar]
  10. Manning M., Mitchell T.G. 1980; Morphogenesis of Candida albicans and cytoplasmic proteins associated with differences in morphology, strain or temperature. Journal of Bacteriology 144:258–273
     [Google Scholar]
  11. Necas O. 1961; Physical conditions as important factors for the regeneration of naked yeast protoplasts. Nature, Lendon 192:580–581
     [Google Scholar]
  12. Necas O. 1962; The mechanism of regeneration of yeast protoplasts. I. Physical conditions. Folia biologica (Praha) 11:97–102
     [Google Scholar]
  13. Nickerson W. J., Swaminathan V. 1977; Linkages in cell wall of yeast. In Second International Mycologica/ Congress Abstracts vol 112 p. 476 Tampa, Florida, USA:
     [Google Scholar]
  14. Notario V. 1982; β-Glucanases from Candida albicans:purification, characterization and the nature of their attachment to cell wall components. Journal of General Microbiology 128:747–759
     [Google Scholar]
  15. Pastor F. I. J., Valentin E., Herrero E., Sentandreu R. 1984; Structure of the Saccharo myces cere1isiaecell wall:mannoproteins released by zymolyase and their contribution to wall architecture. Biochimica et biophysica acta 802:292–300
     [Google Scholar]
  16. Shibata N., Mizugami K., Takano K., Suzuki S. 1983; Isolation ofmannan-protein complexes from viable cells of Saccharomyces cerevisiae X2l80-1A wild type and Saccharomyces cerevisiaeX2180-IA-5 mutant strains by the action of Zymolyase-60000. Journal of Bacteriology 156:552–558
     [Google Scholar]
  17. Somogyi M. 1952; Notes on sugar determination. Journal of Biological Chemistry 195:19–23
     [Google Scholar]
  18. Sullivan P. A., Chjew Y. Y., Molloy C., Templeton M., Shepherd M. G. 1983; An analysis of the metabolism and cell wall composition of Candida albicans during germ tube formation. Canadian Journal of Microbiology 29:1514–1525
     [Google Scholar]
  19. Valentin E., Herrero E., Pastor F. I. J., Sentandreu R. 1984; Solubilization and analysis of mannoprotein molecules from the cell wall of Saccharomyces cerevisiae. Journal ofGeneral Microbiology 130:1419–1428
     [Google Scholar]
  20. Yu R. J., Bishop C.T., Cooper F. P., Hasenclever H. F., Blank F. 1967; Structural studies of mannan from Candida albicans (serotypes A & B), Candida parapsiosis, Candida ste/latoideaandCandida tropicalis. Canadian Journal of Chemistry 45:2205–2211
     [Google Scholar]
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Dimorphism in Candida albicans: Contribution of Mannoproteins to the Architecture of Yeast and Mycelial Cell Walls
Microbiology 131, 2209 (1985); https://doi.org/10.1099/00221287-131-9-2209
/content/journal/micro/10.1099/00221287-131-9-2209
/content/journal/micro/10.1099/00221287-131-9-2209
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