1887

Abstract

Summary: The free lipids of a sample of were extracted by a procedure designed to produce separate non-polar and polar fractions. The composition of these lipids was analysed semi-quantitatively by five special thin-layer chromatographic systems covering the total range of mycobacterial lipid polarities. In order of increasing polarity, the major lipids were dimycocerosates of phthiocerol A, phthiocerol B and phthiodiolone A, glycosyl phenolphthiocerol dimycocerosates and phospholipids, including monoacylphosphatidylinositol di- and pentamannosides. The diacylated forms of these latter lipids, found in most mycobacteria, were not present. The composition of the free lipids of the leprosy bacillus, surveyed over the total polarity range for the first time, showed that the patterns were particularly related to those of and .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-8-2007
1985-08-01
2021-05-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/8/mic-131-8-2007.html?itemId=/content/journal/micro/10.1099/00221287-131-8-2007&mimeType=html&fmt=ahah

References

  1. Asselineau C., Clavel S., Clément F., Daffé M., David H., Lanéelle M. A., Promé J. C. 1981; Constituants lipidiques de Mycobacterium leprae isolé de tatou infecté expérimentalement. Annales de Microbiologie 132A:19–30
    [Google Scholar]
  2. Athwal R. S., Deo S. S., Imaeda T. 1984; Deoxyribonucleic acid relatedness among Mycobacterium leprae, Mycobacterium lepraemurium, and selected bacteria by dot blot and spectrophotometric deoxyribonucleic acid hybridization assays. International Journal of Systematic Bacteriology 34:371–375
    [Google Scholar]
  3. Daffé M., Lanéelle M. A., Roussel J., Asselineau C. 1984; Lipides specifiques de Mycobacterium ulcerans . Annales de Microbiologie 135A:191–201
    [Google Scholar]
  4. Dhariwal K. R., Dhariwal G., Goren M. B. 1984; Observations on the ubiquity of the Mycobacterium tuberculosis sulfatides in mycobacteria. American Review of Respiratory Diseases 130:641–646
    [Google Scholar]
  5. Dobson G., Minnikin D. E., Minnikin S. M., Parlett J. H., Goodfellow M., Ridell M., Magnusson M. 1985; Systematic analysis of complex mycobacterial lipids. In Chemical Methods in Bacterial Svstematics pp 237–265 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  6. Draper P. 1980; Studies on Mycobacterium leprae from infected armadillos. In Leprosy – Proceedings of the 11th International Leprosy Congress Mexico City 1978, pp 97–101 Edited by Latapi F. Excerpta Medica International Congress Series no. 466
    [Google Scholar]
  7. Draper P., Dobson G., Minnikin D. E., Minnikin S. M. 1982; The mycolic acids of Mycobacterium leprae harvested from experimentally infected nine-banded armadillos. Annales de Microbiologie 133B:39–47
    [Google Scholar]
  8. Draper P., Payne S. N., Dobson G., Minnikin D. E. 1983; Isolation of a characteristic phthiocerol dimycocerosate from Mycobacterium leprae . Journal of General Microbiology 129:859–863
    [Google Scholar]
  9. Hunter S. W., Brennan P. J. 1983; Further specific extracellular phenolic glycolipid antigens and a related diacylphthiocerol from Mycobacterium leprae . Journal of Biological Chemistry 258:7556–7562
    [Google Scholar]
  10. Hunter S. W., Fujiwara T., Brennan P. J. 1982; Structure and antigenicity of the major specific glycolipid antigen of Mycobacterium leprae . Journal of Biological Chemistry 257:15072–15078
    [Google Scholar]
  11. Imaeda T., Kirchheimer W. F., Barksdale L. 1982; DNA isolated from Mycobacterium leprae: genome size, base ratio, and homology with other related bacteria as determined by optical DNA–DNA reassociation. Journal of Bacteriology 150:414–417
    [Google Scholar]
  12. Khuller G. K., Malik U., Kumar B. 1982; Antibodies to sulfolipids in human leprosy sera. International Journal of Leprosy 50:319–321
    [Google Scholar]
  13. Minnikin D. E. 1982; Lipids: complex lipids, their chemistry, biosynthesis and roles. In The Biology of the Mycobacteria vol 1 pp 95–184 Edited by Ratledge C., Stanford J. L. London: Academic Press;
    [Google Scholar]
  14. Minnikin D. E., Goodfellow M. 1980; Lipid composition in the classification and identification of acid-fast bacteria. In Microbiological Classification and Identification pp 189–256 Edited by Goodfellow M, Board R. G. London: Academic Press;
    [Google Scholar]
  15. Minnikin D. E., Dobson G., Hutchinson I. G. 1983; Characterization of phthiocerol dimycocerosates from Mycobacterium tuberculosis . Biochimica et biophysica acta 753:445–449
    [Google Scholar]
  16. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. Journal of Microbiological Methods 2:233–241
    [Google Scholar]
  17. Minnikin D. E., Dobson G., Goodfellow M., Draper P., Magnusson M. 1985a; Quantitative comparison of the mycolic and fatty acid composition of Mycobacterium leprae and Mycobacterium gordonae . Journal of General Microbiology 131:2013–2021
    [Google Scholar]
  18. Minnikin D. E., Dobson G., Goodfellow M., Magnusson M., Ridell M. 1985b; Distribution of some mycobacterial waxes based on the phthiocerol family. Journal of General Microbiology 131:1375–1381
    [Google Scholar]
  19. Minnikin D. E., Dobson G., Sesardic D., Ridell M. 1985c; Mycolipenates and mycolipanolates of trehalose from Mycobacterium tuberculosis . Journal of General Microbiology 131:1369–1374
    [Google Scholar]
  20. Prabhudesai A. V., Kaur S., Khuller G. K. 1981; Sulpholipids of Nocardia species – a preliminary report. Indian Journal of Medical Research 73:181–183
    [Google Scholar]
  21. World Health Organization 1975; Proposed system for preparing purified suspensions of M. leprae from tissues of infected armadillos. Leprosy Review 47:313–332
    [Google Scholar]
  22. Young D. B. 1981; Detection of mycobacterial lipids in skin biopsies from leprosy patients. International Journal of Leprosy 49:198–204
    [Google Scholar]
  23. Young D. B. 1982; Mycobacterial lipids in infected tissue samples. Annales de Microbiologie 133B:53–58
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-8-2007
Loading
/content/journal/micro/10.1099/00221287-131-8-2007
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error