1887

Abstract

Summary: Capsular polysaccharide from two strains of serotype T15 was purified and characterized by chemical analysis and NMR spectroscopy. The polymer, a teichoic acid, proved to be very similar in structure to the capsular polysaccharide of serotype T4 and identical to the previously described K62 (K2ab) capsular polysaccharide of . and the capsular polysaccharide of serotype H, i.e. (2-glycerol-3) (phosphate) (4-x-D-galactopyranose-1) with partial -acetylation on the galactose residues. Electron microscopy with Protein A-gold labelled antisera showed that the polysaccharide was peripherally located on the surface of all three organisms. Chemical removal of -acetyl groups from the polysaccharide yielded a structure identical to that previously described for K2 (K2a). Both -acetylated and de--acetylated T15 polymers, when absorbed on to sheep erythroyctes in passive haemagglutination assays, yielded identical antibody titres with sera raised against T15, K 2 or H whole cells. De--acetylation of the Pasteurella polysaccharide influenced its precipitability with immune sera, but this could not be related to the absence of -acetyl groups because the non-acetylated K2 polymer readily precipitated with a line of ‘identity’ with the acetylated T15 polymer.

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/content/journal/micro/10.1099/00221287-131-8-1963
1985-08-01
2021-05-13
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