1887

Abstract

Summary: Three benzylpenicillin-resistant, clinical isolates of (MIC values 16-64 μ;g ml) contained six penicillin-binding proteins (PBPs), of which PBP5 was the most abundant and had the lowest affinity for the antibiotic. Four benzylpenicillin-susceptible strains (MIC values 0·031-0·5 μ;g ml) were obtained as spontaneous derivatives from these above organisms. There were significant decreases in the amounts of PBP5 in each of the derivatives, with the concomitant appearance of a new, higher affinity PBP (5) in three strains. Increased amounts of PBP5, with no changes in PBP5, were found in several mutants with intermediate-level benzylpenicillin-resistance (MIC values 1-8 μ;g ml) selected from two of the susceptible strains. Examination of 18 other clinical isolates, with a wide range of susceptibilities to benzylpenicillin (MIC values 0·062-128 μ;g ml), showed that PBP5 was present in 13 strains, and PBP5 in all of them, but in differing amounts. The results concerning the relative amounts and relative affinities of PBPs 5 and 5 allowed the categorization of the various strains into six groups, within which organisms had somewhat similar susceptibilities to benzylpenicillin.

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/content/journal/micro/10.1099/00221287-131-8-1933
1985-08-01
2021-05-17
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References

  1. Coyette J., Ghuysen J. -M., Fontana R. 1980; The penicillin-binding proteins in Streptococcus faecalis ATCC 9790. European Journal of Biochemistry 110:445–456
    [Google Scholar]
  2. Daneo-Moore L., Pucci M. 1982; Penicillin tolerance and resistance in Streptococcus faecium . In Microbiology – 1982 pp 199–203 Edited by Schlessinger D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. Daneo-Moore L., Pucci M., Zito E., Ferrero M. 1983; Studies of β-lactam resistance in Streptococcus faecium . In Target of Penicillin pp 493–497 Edited by Hakenbeck R., Höltje J. -V., Labischinski H. Berlin: Walter de Gruyter;
    [Google Scholar]
  4. Eliopoulos G. M., Wennersten C., Moellering R. C. Jr 1982; Resistance to β-lactam antibiotics in Streptococcus faecium . Antimicrobial Agents and Chemotherapy 22:295–301
    [Google Scholar]
  5. Facklam R. R. 1972; Recognition of group D streptococcal species of human origin by biochemical and physiological tests. Applied Microbiology 23:1131–1139
    [Google Scholar]
  6. Fontana R., Cerini R., Longoni P., Grossato A., Canepari P. 1983; Identification of a streptococcal penicillin-binding protein that reacts very slowly with penicillin. Journal of Bacteriology 155:1343–1350
    [Google Scholar]
  7. Handwerger S., Tomasz A. 1984; Both concentration and affinity of PBPs change in pneumococcal penicillin resistance. In Program and Abstracts. 24th Interscience Conference on Antimicrobial Agents and Chemotherapy abstract 1000 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Horodniceanu T., Delbos F. 1980; Group D streptococci in human infections: identification and sensitivity to antibiotics. Annales de Microbiologie 131B:131–144
    [Google Scholar]
  9. Horodniceanu T., Bouanchaud D. H., Bieth G., Chabbert Y. A. 1976; R plasmids in Streptococcus agalactiae (group B). Antimicrobial Agents and Chemotherapy 10:795–801
    [Google Scholar]
  10. Le Bouguénec C., Horodniceanu T. 1982; Conjugative R plasmids in Streptococcus faecium (group D). Antimicrobial Agents and Chemotherapy 21:698–705
    [Google Scholar]
  11. Weinstein A. J., Moellering R. C. Jr 1975; Studies of cephalothin: aminoglycoside synergism against enterococci. Antimicrobial Agents and Chemotherapy 7:522–529
    [Google Scholar]
  12. Williamson R., Calderwood S. B., Moellering R. C. Jr, Tomasz A. 1983; Studies on the mechanism of intrinsic resistance to β-lactam antibiotics in group D streptococci. Journal of General Microbiology 129:813–822
    [Google Scholar]
  13. Zighelboim S., Tomasz A. 1980; Penicillin-binding proteins of multiply antibiotic-resistant South African strains of Streptococcus pneumoniae . Antimicrobial Agents and Chemotherapy 17:434–442
    [Google Scholar]
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