Summary: The gene of plays a key role in co-ordinating cell proliferation and nutrient availability. A 2·6 kb yeast DNA sequence has been cloned which fully suppresses the mutation. Integration of this sequence to demonstrate that the structural gene itself had been cloned proved difficult. Integration occurred only rarely and only at the locus which was also present on the integrating plasmid. To circumvent these difficulties an adjacent sequence, present on the original isolate from the gene library, was subcloned onto the integrating vector YIp5, after which directed integration proved straightforward. The integrated sequence was closely linked to , confirming that the structural gene had been cloned. A chromosomal restriction map of the region is presented; no gross changes were observed in the region as cells entered stationary phase.


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