Summary: Regulatory genes controlling expression and also promoters fused to from were cloned on wide-host-range plasmids and introduced into to compare regulation of expression in the two organisms. A low-copy-number plasmid carrying corrected an Nifregulatory mutation, whereas a plasmid carrying did not. A high-copy-number plasmid carrying eliminated expression in but not in and fusions were expressed strongly in and were not repressed by ammonium. fusion was not expressed in any conditions in this background except very weakly when was also present. The implications of these findings are discussed.


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