Summary: We have previously reported that has multiple copies of nitrogen-fixation gene sequences. In this work we extend our analysis to cover a broader range of strains and other rhizobia isolated from different legumes. Our results indicate that most strains have reiterated sequences. Reiterations are also found in rhizobia from nodules of other species and from the close relative of However, gene reiteration is not always found in the rhizobia able to nodulate and fix nitrogen in , since some strains isolated from nodules of as well as from other legumes are able to establish an effective symbiosis with and do not show reiterations. We propose that there are different evolutionary lines of The reiteration of genes may be considered a marker for the most abundant and specialized of these evolutionary lines.


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