1887

Abstract

Summary: Two monoclonal antibodies specific for type-1C fimbriae of were produced. In enzyme-linked immunosorbent assay and immunoblotting the antibodies, which were of the IgG1 isotype, reacted with type-1C, but not with P or type-1 fimbriae of strain KS71. Immunoblotting and immunoprecipitation of crude fimbrial extracts from 25 strains invariably gave an apparent molecular weight of 17000 for the type-1C fimbrillin. A total of 313 strains, isolated from patients with extraintestinal infection or from faeces of healthy children, were screened for the presence of type-1C fimbriae using both the monoclonal and polyclonal antibodies. Of these, 45 (14%) strains had type-1C fimbriae, with the highest frequency (27%) on strains isolated from patients with pyelonephritis. No faecal strain had type-1C fimbriae, and the frequency on the other diagnostic groups ranged from 11 to 15%. Thus, no direct correlation between type-1C fimbriae and bacterial virulence in human extraintestinal infections was found. Type-1C fimbriae were detected on only a few serotypes, notably on all O6:K2:H1 and O22:K13:H1 strains tested.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-7-1705
1985-07-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/7/mic-131-7-1705.html?itemId=/content/journal/micro/10.1099/00221287-131-7-1705&mimeType=html&fmt=ahah

References

  1. Achtman M., Mercer A., Kusecek B., Pohl A., Heuzenroeder M., Aaronson W., Sutton A., Silver R. P. 1983; Six widespread bacterial clones among Escherichia coli K1 isolates. Infection and Immunity 39:315–335
    [Google Scholar]
  2. Duguid J. P., Old D. C. 1980; Adhesive properties of Enterobacteriaceae . In Bacterial Adherence. Receptors and Recognition series B, vol 6 pp 185–217 Edited by Beachey E. H. London: Chapman & Hall;
    [Google Scholar]
  3. Engvall E., Perlman P. 1972; Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. Journal of Immunology 109:129–135
    [Google Scholar]
  4. Gaastra W., de Graaf F. K. 1982; Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains. Microbiological Reviews 46:129–161
    [Google Scholar]
  5. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. 1977; Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature, London 266:550–552
    [Google Scholar]
  6. Hermodson M. A., Chen K. C. S., Buchanan T. M. 1978; Neisseria pili proteins: amino-terminal sequence and identification of an unusual amino acid. Biochemistry 17:442–445
    [Google Scholar]
  7. Källenius G., Svenson S. B., Mölby R., Cedergren B., Hultberg H., Wlndberg J. 1981; Structure of carbohydrate part of receptor on human uroepithelial cells for pyelonephritogenic Escherichia coli . Lancet ii:604–606
    [Google Scholar]
  8. Klemm P., Ørskov I., Ørskov F. 1982; F7 and type 1-like fimbriae from three Escherichia coli strains isolated from urinary tract infections: protein chemical and immunological aspects. Infection and Immunity 36:462–468
    [Google Scholar]
  9. Korhonen T. K., Edén S., Svanborg Edén C. 1980; Binding of purified Escherichia coli pili to human urinary tract epithelial cells. FEMS Microbiology Letters 7:237–240
    [Google Scholar]
  10. Korhonen T. K., Väisänen V., Saxen H., Hultberg H., Svenson S. B. 1982; P-antigen-recognizing fimbriae from human uropathogenic Escherichia coli strains. Infection and Immunity 37:286–291
    [Google Scholar]
  11. Korhonen T. K., Väisänen-Rhen V., Rhen M., Pere A., Parkkinen J., Finne J. 1984; Escherichia coli fimbriae recognizing sialyl galactosides. Journal of Bacteriology 159:762–766
    [Google Scholar]
  12. Korhonen T. K., Valtonen M. V., Parkkinen J., Väisänen-Rhen V., Finne J., Ørskov F., Ørskov I., Svenson S. B., Mäkelä P. H. 1985; Serotypes, hemolysin production and receptor recognition of Escherichia coli strains associated with neonatal sepsis and meningitis. Infection and Immunity 48: (in the Press)
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Leffler H., Svanborg Edén C. 1980; Chemical identification of a glycosphingolipid receptor for Escherichia coli attaching to human urinary tract epithelial cells and agglutinating human erythrocytes. FEMS Microbiology Letters 8:127–134
    [Google Scholar]
  15. Littlefield J. W. 1964; Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145:709–710
    [Google Scholar]
  16. Nowicki B., Rhen M., Väisänen-Rhen V., Pere A., Korhonen T. K. 1984; Immunofluorescence study of fimbrial phase variation in Escherichia coli KS71. Journal of Bacteriology 160:691–695
    [Google Scholar]
  17. Ørskov I., Ørskov F., Birch-Andersen A., Klemm P., Svanborg Edén C. 1982; Protein attachment factors: fimbriae in adhering Escherichia coli strains. In Seminars in Infectious Diseases. Volume IV. Bacterial Vaccines pp 97–103 Edited by Robbins J. B., Hill J. C., Sadoff J. C. New York: Thieme–Stratton;
    [Google Scholar]
  18. Parkkinen J., Finne J., Achtman M., Väisänen V., Korhonen T. K. 1983; Escherichia coli strains binding neuraminyl α2-3 galactosides. Biochemical and Biophysical Research Communications 111:456–461
    [Google Scholar]
  19. Rhen M. 1985; Characterization of DNA fragments encoding fimbriae of the uropathogenic Escherichia coli strain KS71. Journal of General Microbiology 131:571–580
    [Google Scholar]
  20. Rhen M., Wahlström E., Korhonen T. K. 1983a; P-fimbriae of Escherichia coli: fractionation by immune precipitation. FEMS Microbiology Letters 18:227–232
    [Google Scholar]
  21. Rhen M., Klemm P., Wahlström E., Svenson S. B., Källenius G., Korhonen T. K. 1983b; P-fimbriae of Escherichia coli: immuno- and protein-chemical characterization of fimbriae from two pyelonephritogenic strains. FEMS Microbiology Letters 18:233–238
    [Google Scholar]
  22. Rhen M., Knowles J., Penttilä M. E., Sarvas M., Korhonen T. K. 1983c; P-fimbriae of Escherichia coli: molecular cloning of DNA fragments containing the structural genes. FEMS Microbiology Letters 19:119–123
    [Google Scholar]
  23. Rhen M., Mäkelä, P. H. & Korhonen T. K. 1983d; P-fimbriae of Escherichia coli are subject to phase variation. FEMS Microbiology Letters 19:267–271
    [Google Scholar]
  24. Salit I. E., Gotschlich E. C. 1977; Hemagglutination by purified type 1 Escherichia coli pili. Journal of Experimental Medicine 146:169–1181
    [Google Scholar]
  25. Svanborg Edén C., Hanson L. Å., Jodal U., Lindberg U., Sohl-Åkerlund A. 1976; Variable adhesion to normal urinary tract epithelial cells of Escherichia coli strains associated with various forms of urinary tract infection. Lancet ii:490–492
    [Google Scholar]
  26. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrosellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  27. Väisänen V., Elo J., Tallgren L. G., Siitonen A., Mäkelä P. H., Svanborg Edén C., Källenius G., Svenson S. B., Hultberg H., Korhonen T. K. 1981; Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis. Lancet ii:1366–1369
    [Google Scholar]
  28. Väisänen V., Korhonen T. K., Jokinen M., Gahmberg G. C., Ehnholm C. 1982; Blood group M specific hemagglutinin in pyelonephritogenic Escherichia coli . Lancet i:1192
    [Google Scholar]
  29. Väisänen-Rhen V., Elo J., Väisänen E., Siitonen A., Ørskov I., Ørskov F., Svenson S. B., Mäkelä, P. H. & Korhonen T. K. 1984; P-fimbriated clones among uropathogenic Escherichia coli strains. Infection and Immunity 43:149–155
    [Google Scholar]
/content/journal/micro/10.1099/00221287-131-7-1705
Loading
/content/journal/micro/10.1099/00221287-131-7-1705
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error