1887

Abstract

To examine one possible mechanism of damage to leucocytes and tissue cells in legionellosis, seven species of were examined for cytolytic activity and for elaboration of phospholipase C, an enzyme that can damage mammalian cell membranes. Cytolysis was assessed using erythrocytes in agar. Phospholipase C was assayed by release of -nitrophenol from -nitrophenylphosphorylcholine and of tritiated phosphorylcholine from -α-dipalmitoyl-[-H]phosphatidylcholine. and all lysed dog red blood cells, which have a high ratio of membrane phosphatidylcholine to sphingomyelin. The same strains hydrolysed varying amounts of -nitrophenylphosphorylcholine; exhibited the greatest activity. and , but not , released tritiated phosphorylcholine from labelled substrate. These results indicate that several species of possess cytolytic capability; exotoxins with phospholipase C activity may play a role.

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1985-06-01
2022-07-02
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References

  1. Ahkong Q. F., Fisher D., Tampion W., Lucy J. A. 1973; The fusion of erythrocytes by fatty acids, esters, retinol and α-tocopherol. Biochemical Journal 136:147–155
    [Google Scholar]
  2. Allan D., Low M. G., Finean J. B., Michell R. H. 1975; Changes in lipid metabolism and cell morphology following attack by phospholipase C (Clostridium perfringens) on red cells or lymphocytes. Biochimica et biophysica acta 413:309–316
    [Google Scholar]
  3. Allan D., Billah M. M., Finean J. B., Michell R. H. 1976; Release of diacylglycerol-enriched vesicles from erythrocytes with increased intracellular [Ca2+]. Nature, London 261:58–60
    [Google Scholar]
  4. Allen S. D., Siders J. A. 1980; Procedures for isolation and characterization of anaerobic bacteria. In Manual of Clinical Microbiology, 3rd.397–417 Lennette E. H., Balows A., Hausler W. J. Jr, Truant J. P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Alouf J. E. 1977; Cell membranes and cytolytic bacterial toxins. In The Specificity and Action of Animal., Bacterial and Plant Toxins219–270 Cuatrecasas P. London: Chapman & Hall;
    [Google Scholar]
  6. Arbuthnott J. P. 1982; Bacterial cytolysins (membrane-damaging toxins). In Molecular Action of Toxins and Viruses107–129 Cohen P., Van Heyningen S. Amsterdam: Elsevier Biomedical Press;
    [Google Scholar]
  7. Avigad G. 1976; Microbial phospholipases. In Mechanisms in Bacterial Toxinology99–167 Bernheimer A. W. New York: John Wiley & Sons;
    [Google Scholar]
  8. Baine W. B., Rasheed J. K., Mackel D. C., Bopp C. A., Wells J. G., Kaufmann A. F. 1979a; Exotoxin activity associated with the Legionnaires disease bacterium. Journal of Clinical Microbiology 9:453–456
    [Google Scholar]
  9. Baine W. B., Rasheed J. K., Maca H. W., Kaufmann A. F. 1979b; Hemolytic activity of plasma and urine from rabbits experimentally infected with Legionella pneumophila . Reviews of Infectious Diseases 1:912–917
    [Google Scholar]
  10. Bayer A. S., Nelson S. C., Galpin J. E., Chow A. W., Guze L. B. 1975; Necrotizing pneumonia and empyema due to Clostridium perfringens . American Journal of Medicine 59:851–856
    [Google Scholar]
  11. Berdal B. P., Bovre K., Olsvik Ø., Omland T. 1983; Patterns of extracellular proline-specific endopeptidases in Legionella and Flavobacterium spp. demonstrated by use of chromogenic peptides. Journal of Clinical Microbiology 17:970–974
    [Google Scholar]
  12. Blackmon J. A., Chandler F. W., Cherry W. B., England A. C. III, Feeley J. C., Hicklin M. D., McKinney R. M., Wilkinson H. W. 1981; Legionellosis. American Journal of Pathology 103:427–465
    [Google Scholar]
  13. Chrisope G. L., Fox C. W., Marshall R. T. 1976; Lecithin agar for detection of microbial phospholipases. Applied and Environmental Microbiology 31:784–786
    [Google Scholar]
  14. Edwards D., Finlayson D. M. 1980; Legionnaires’ disease causing severe lung abscesses. Canadian Medical Association Journal 123:524–527
    [Google Scholar]
  15. Feeley J. C., Gorman G. W., Weaver R. E., Mackel D. C., Smith H. W. 1978; Primary isolation media for Legionnaires disease bacterium. Journal of Clinical Microbiology 8:320–325
    [Google Scholar]
  16. Feeley J. C., Gibson R. J., Gorman G. W., Langford N. C., Rasheed J. K., Mackel D. C., Baine W. B. 1979; Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila . Journal of Clinical Microbiology 10:437–441
    [Google Scholar]
  17. Finnerty W. R., Makula R. A., Feeley J. C. 1979; Cellular lipids of the Legionnaires’ disease bacterium. Annals of Internal Medicine 90:631–634
    [Google Scholar]
  18. Friedman R. L., Iglewski B. H., Miller R. D. 1980; Identification of a cytotoxin produced by Legionella pneumophila . Infection and Immunity 29:271–274
    [Google Scholar]
  19. Friedman R. L., Lochner J. E., Bigley R. H., Iglewski B. H. 1982; The effects of Legionella pneumophila toxin on oxidative processes and bacterial killing of human polymorphonuclear leukocytes. Journal of Infectious Diseases 146:328–334
    [Google Scholar]
  20. Frisch C. F., Baine W. B. 1984; Production of extracellular hemolytic cytotoxin by Legionella bozemanii . Microbiology and Immunology 28:1267–1272
    [Google Scholar]
  21. Grossman S., Oestreicher G., Hogue P. K., Cobley J. G., Singer T. P. 1974; Microanalytical determination of the activities of phospholipases A, C, and D and of their mixtures. Analytical Biochemistry 58:301–309
    [Google Scholar]
  22. Gulewitsch W. 1898; Uber Cholin und einige Verbindungen desselben. Zeitschrift für Physiologische Chemie 24:513–541
    [Google Scholar]
  23. Hebert G. A., Steigerwalt A. G., Brenner D. J. 1980; Legionella micdadei species nova: classification of a third species of Legionella associated with human pneumonia. Current Microbiology 3:255–257
    [Google Scholar]
  24. Horwitz M. A. 1983; The Legionnaires’ disease bacterium (Legionella pneumophila) inhibits phago-some-lysosome fusion in human monocytes. Journal of Experimental Medicine 158:2108–2126
    [Google Scholar]
  25. Horwitz M. A., Silverstein S. C. 1980; Legionnaires’ disease bacterium (Legionella pneumophila) multiplies intracellularly in human monocytes. Journal of Clinical Investigation 66:441–450
    [Google Scholar]
  26. Horwitz M. A., Silverstein S. C. 1981; Interaction of the Legionnaires’ disease bacterium (Legionellapneumophila) with human phagocytes. II. Antibody promotes binding of L. pneumophila to monocytes but does not inhibit intracellular multiplication. Journal of Experimental Medicine 153:398–406
    [Google Scholar]
  27. Kennerly D. A., Sullivan T. J., Sylwester P., Parker C. W. 1979; Diacylglycerol metabolism in mast cells: a potential role in membrane fusion and arachidonic acid release. Journal of Experimental Medicine 150:1039–1044
    [Google Scholar]
  28. Klaus M. H., Clements J. A., Havel R. J. 1961; Composition of surface-active material isolated from beef lung. Proceedings of the National Academy of Sciences of the United States of America 471858–1859
    [Google Scholar]
  29. Kurioka S., Matsuda M. 1976; Phospholipase C assay using p-nitrophenylphosphorylcholine together with sorbitol and its application to studying the metal and detergent requirement of the enzyme. Analytical Biochemistry 75:281–289
    [Google Scholar]
  30. Lehninger A. L. 1975 Biochemistry, 2nd.303 New York: Worth Publishers;
    [Google Scholar]
  31. Lewin S., Brettman L. R., Goldstein E. J. C., Holzman R. S., Devila H., Taubman F., Sierra M. F., Edelstein P. H. 1979; Legionnaires’ disease: a cause of severe abscess-forming pneumonia. American Journal of Medicine 67:339–342
    [Google Scholar]
  32. Liu P. V. 1977; Exotoxins of gram-negative bacteria exclusive of enterotoxins. In Perspectives in Toxinology43–60 Bernheimer A. W. New York: John Wiley & Sons;
    [Google Scholar]
  33. MacLennan J. D. 1962; The histotoxic clostridial infections of man. Bacteriological Reviews 26:177–276
    [Google Scholar]
  34. Magnussen C. R., Israel R. H. 1980; Legionnaires’ lung abscess. American Journal of the Medical Sciences 279:117–120
    [Google Scholar]
  35. Meyer R. D. 1983; Legionella infections: a review of five years of research. Reviews of Infectious Diseases 5:258–278
    [Google Scholar]
  36. Möllby R. 1978; Bacterial phospholipases. In Bacterial Toxins and Cell Membranes367–424 Jeljaszewicz J., Wadstrom T. New York: Academic Press;
    [Google Scholar]
  37. Muller H. E. 1981; Enzymatic profile of Legionella pneumophila . Journal of Clinical Microbiology 13:423–426
    [Google Scholar]
  38. Nash T. W., Libby D. M., Horwitz M. A. 1982; Legionnaires’ disease bacteria multiply in human alveolar macrophages (AM) and activated alveolar macrophages inhibit multiplication [abstract no. 93]. In Program and Abstracts of the 22nd Interscience Conference on Antimicrobial Agents and ChemotherapyWashington, DC American Society for Microbiology;
    [Google Scholar]
  39. Nolte F. S., Hollick G. E., Robertson R. G. 1982; Enzymatic activities of Legionella pneumo. phila and Legionella-Yike organisms. Journal of Clinical Microbiology 15:175–177
    [Google Scholar]
  40. Pasculle A. W., Feeley J. C., Gibson R. J., Cordes L. G., Myerowitz R. L., Patton C. M., Gorman G. W., Carmack C. L., Ezzell J. W., Dowling J. N. 1980; Pittsburgh pneumonia agent: direct isolation from human lung tissue. Journal of Infectious Diseases 141:727–732
    [Google Scholar]
  41. Renshaw R. R. 1910; Preparation of choline and some of its salts. Journal of the American Chemical Society 32:128–130
    [Google Scholar]
  42. Scarpelli E. M. 1968 The Surfactant System of the Lung269 Philadelphia: Lea & Febiger;
    [Google Scholar]
  43. Smith L. D. S., Dowell V. R. Jr, Allen S. D. 1980; Clostridium. In Manual of Clinical Microbiology, 3rd.418–425 Lennette E. H., Balows A., Hausler W. J. Jr, Truant J. P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  44. Sullivan T. J. 1981; Diacylglycerol metabolism and the release of mediators from mast cells. In Biochemistry of the Acute Allergic Reactions229–238 Becker E. L., Simon A. S., Austen K. F. New York: Alan R. Liss;
    [Google Scholar]
  45. Taguchi R., Mizuno M., Inoue M., Ikezawa H. 1983; Increase in osmotic fragility of bovine erythrocytes induced by bacterial phospholipases C. Journal of Biochemistry 93:403–412
    [Google Scholar]
  46. Thompson M. R., Miller R. D., Iglewski B. H. 1981; In vitro production of an extracellular protease by Legionella pneumophila . Infection and Immunity 34:299–302
    [Google Scholar]
  47. Thorpe T. C., Miller R. D. 1981; Extracellular enzymes of Legionella pneumophila . Infection and immunity 33:632–635
    [Google Scholar]
  48. Thorpe T. C., Miller R. D. 1984; Characterization of an extracellular hemolysin from Legionella pneumophila . In Legionella: Proceedings of the 2nd International Symposium97–98 Thornsberry C., Balows A., Feeley J. C., Jakubowski W. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  49. Turner J. C., Anderson H. M., Gandal C. P. 1958; Species differences in red blood cell phosphatides separated by column and paper chromatography. Biochimica et biophysica acta 30:130–134
    [Google Scholar]
  50. Wilkinson P. C. 1975; Inhibition of leukocyte locomotion and chemotaxis by lipid-specific bacterial toxins. Nature, London 255:485–487
    [Google Scholar]
  51. Wilkinson P. C., Allan R. B. 1978; Binding of protein chemotactic factors to the surfaces of neutrophil leukocytes and its modification with lipid-specific bacterial toxins. Molecular and Cellular Biochemistry 20:25–40
    [Google Scholar]
  52. Winn W. C. Jr, Myerowitz R. L. 1981; The pathology of the Legionella pneumonias. Human Pathology 12:401–422
    [Google Scholar]
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