1887

Abstract

The strongest of five ‘early’ promoters of phage SPP1 was localized in a DNA restriction fragment by analysis of RNA polymerase binding and R-loop formation. The nucleotide sequence of the promoter region was established. The signal structures identified were similar to those recognized by the RNA polymerase of . . The promoter precedes an open reading frame with 51 codons. A protein with the predicted from the nucleotide sequence was identified in minicells.

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/content/journal/micro/10.1099/00221287-131-5-1259
1985-05-01
2024-05-06
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References

  1. Amann E., Reeve J. N. 1981; Cloning and expression of the Bacillus subtilis phage SPP1 in E. coli: II. Gene expression of λ/SPPl hybrid phages in E. coli minicells and the identification of SPP1 polypeptides. Molecular and General Genetics 182:299–303
    [Google Scholar]
  2. Behrens B., Pawlek B., Morelli G., Trautner T. A. 1983; Restriction and modification in Bacillus subtilis: construction of hybrid λ and SPP1 phages containing a DNA methyltransferase gene from B. subtilis phage SPR. Molecular and General Genetics 189:10–16
    [Google Scholar]
  3. Brack C. 1979; Electron microscopic analysis of transcription: mapping of initiation sites and direction of transcription. Proceedings of the National Academy of Sciences of the United States of America 763164–3168
    [Google Scholar]
  4. Dougan G., Sherratt D. 1977; The transposon Tnl as a probe for studying ColEl structure and function. Molecular and General Genetics 151:151–160
    [Google Scholar]
  5. Esche H. 1975; Gene expression of bacteriophage SPP1. II. Regulatory aspects. Molecular and General Genetics 142:57–66
    [Google Scholar]
  6. Flock J. I., Fotheringham J., Light J., Bell L., Derbyshire R. 1984; Expression in Bacillus subtilis of the gene for human urogastrone using synthetic ribosome binding sites. Molecular and General Genetics 195:246–251
    [Google Scholar]
  7. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 111680–685
    [Google Scholar]
  8. Maxam A. M., Gilbert W. 1977; A new method for sequencing DNA. Proceedings of the National Academy of Sciences of the United States of America 79560–564
    [Google Scholar]
  9. Mertens G., Reeve J. N. 1977; Synthesis of cell envelope components by anucleate cells (minicells) of Bacillus subtilis. Journal of Bacteriology 129:1198–1207
    [Google Scholar]
  10. Montenegro M. A., Trautner T. A. 1981; In υivo transcription of Bacillus subtilis bacteriophage SPP1. Molecular and General Genetics 181:512–517
    [Google Scholar]
  11. Moran C. P. Jr, Land N., Le Grice S. F. J., Lee G., Stephens M., Sonenshein A. L., Pero J., Losick P. 1982; Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Molecular and General Genetics 186:339–346
    [Google Scholar]
  12. Morelli G., Montenegro M. A., Hillenbrandt G., Scherzinger E., Trautner T. A. 1978; The genome of B. subtilis phage SPP1: assignment of 5'-3'-polarity to the complementary strands of SPP1 DNA. Molecular and General Genetics 164:93–97
    [Google Scholar]
  13. Morelli G., Buhk H.-J., Fisseau C, Lother H., Yoshinaga K., Messer W. 1981; Promoters in the region of the E. coli replication origin. Molecular and General Genetics 184:255–259
    [Google Scholar]
  14. StÜber D., Morelli G., Bujard H., Montenegro M. A., Trautner T. A. 1981; Promoter sites in the genome of B. subtilis phage SPP1. Molecular and General Genetics 181:518–521
    [Google Scholar]
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