1887

Abstract

We have observed plasmid fusions in a background mediated by a single end of Tn. These occur when transposase is provided either in or in . Insertions of the plasmid carrying the TnA inverted repeat sequence occur at many sites in the target plasmid. The point of fusion on the plasmid carrying TnA sequences always appears to be located in the region which carries the TnA inverted repeat sequence. In contrast to the transposition of an intact Tn element, plasmid fusions mediated by one end of Tn are very rare events. The implications of our results for models of transposition are discussed.

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1985-05-01
2021-10-27
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