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Volume 131, Issue 4

Effect of Oxygen Concentration on the Growth and Respiratory Efficiency of Free

Abstract

Maximum molar growth yields on oxygen ( ) and succinate ( ) were not affected when carbon-limited NCIB 8250 was changed to oxygen limitation.→H/O quotients for cells grown with succinate- or ammonium-limitation did not differ significantly from those obtained with oxygen-limited cells. The levels of cytochromes and remained constant at 0‧13 and 0‧05 nmol (mg protein) respectively in crude cell-free extracts of succinate-limited organisms grown at high (80 mmHg) or low (5–10 mmHg) dissolved oxygen tension respectively. Their levels increased in oxygen-limited conditions, when cytochrome () was additionally synthesized and organisms exhibited increased resistance to cyanide inhibition of respiration. It is concluded that the respiratory efficiency of NCIB 8250 is unaffected by oxygen concentration under the conditions studied. Theoretical calculations are presented for maximum molar growth yields on succinate and oxygen and these are compared with experimental values. Although the effective P/O ratio is only about unity, energy is probably conserved at two sites of oxidative phosphorylation and this is independent of the synthesis of cytochrome () under conditions of oxygen limitation. Possible reasons for the observed low molar growth yields are discussed.

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1985-04-01
2024-05-10
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References

  1. Ackrell B. A. C., Jones C. W. 1971a; The respiratory system of Azotobdcter vinelandii. 1. Properties of phosphorylating respiratory membranes. European Journal of Biochemistry 20:22–28
     [Google Scholar]
  2. Ackrell B. A. C., Jones C. W. 1971b; The respiratory system of Azotobacter vinelandii. 2. Oxygen effects. European Journal of Biochemistry 20:29–35
     [Google Scholar]
  3. Asperger O., Kleber H. P., Aurich H. 1978; Cytochrome composition of Acinetobacter calcoaceticus. Acta biologica et medica germanica 37:191–198
     [Google Scholar]
  4. Bauchop T., Elsden S. R. 1960; The growth of microorganisms in relation to their energy supply. Journal of General Microbiology 23:457–469
     [Google Scholar]
  5. Beggs J. D., Fewson C. A. 1977; Regulation of synthesis of benzyl alcohol dehydrogenase in Acinetobacter calcoaceticus NCIB 8250. Journal of General Microbiology 103:127–140
     [Google Scholar]
  6. Carter I. S., Dawes E. A. 1979; Effect of oxygen concentration and growth rate on glucose metabolism, poly-β-hydroxybutyrate biosynthesis and respiration of Azotobacter beijerinckii. Journal of General Microbiology 110:393–400
     [Google Scholar]
  7. Chaney A. L., Marbach E. P. 1962; Modified reagents for determination of urea and ammonia. Clinical Chemistry 8:130–132
     [Google Scholar]
  8. Dalton H., Postgate J. R. 1969; Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous culture. Journal of General Microbiology 54:463–473
     [Google Scholar]
  9. Drozd J., Postgate J. R. 1970; Effects of oxygen on acetylene reduction, cytochrome content and respiratory activity of Azotobacter chroococcum. Journal of General Microbiology 63:63–73
     [Google Scholar]
  10. Ensley B. D., Finnerty W. R. 1980; Influences of growth substrates and oxygen on the electron transport system in Acinetobacter sp. HO1-N. Journal of Bacteriology 142:859–868
     [Google Scholar]
  11. Ensley B. D., Irwin R. M., Carreira L. A., Hoffman P. S., Morgan T. V., Finnerty W. R. 1981; Effects of growth substrates and respiratory chain composition on bioenergetics in Acinetobacter sp. strain HO1-N. Journal of Bacteriology 148:508–513
     [Google Scholar]
  12. Fewson C. A. 1985; Growth yields and respiratory efficiency of Acinetobacter calcoaceticus. Journal of General Microbiology 131:865–872
     [Google Scholar]
  13. Harrison D. E. F. 1973; Growth, oxygen, and respiration. CRC Critical Reviews in Microbiology 2:185–228
     [Google Scholar]
  14. Harrison D. E. F. 1976; The regulation of respiration rate in growing bacteria. Advances in Microbial Physiology 14:243–313
     [Google Scholar]
  15. Harrison D. E. F., Loveless J. E. 1971; The effect of growth conditions on respiratory activity and growth efficiency in facultative anaerobes grown in chemostat culture. Journal of General Microbiology 68:35–43
     [Google Scholar]
  16. Herbert D., Phipps P. J., Tempest D. W. 1965; The chemostat: design and instrumentation. Laboratory Practice 14:1150–1161
     [Google Scholar]
  17. Holdeman L. V., Cato E. P., Moore W. E. C. 1977 Anaerobe Laboratory Manual, 4th.134–135 Blacksburg: Virginia Polytechnic Institute and State University;
     [Google Scholar]
  18. Holz G., Bergmeyer H. U. 1978; In Principles of Enzymatic Analysis. 3 Section D, Methods for Determination of Metabolites1528–1532 Bergmeyer H. U. London: Academic Press;
     [Google Scholar]
  19. Jackson F. A., Dawes E. A. 1976; Regulation of the tricarboxylic acid cycle and poly-β-Miydroxybu-tyrate metabolism in Azotobacter beijerinckii grown under nitrogen or oxygen limitation. Journal of General Microbiology 97:303–312
     [Google Scholar]
  20. Jones C. W., Brice J. M., Downs A. J., Drozd J. W. 1975; Bacterial respiration-linked proton translocation and its relationship to respiratory-chain composition. European Journal of Biochemistry 52:265–271
     [Google Scholar]
  21. Jones C. W., Brice J. M., Edwards C. 1977; The effect of respiratory chain composition on the growth efficiencies of aerobic bacteria. Archives of Microbiology 115:85–93
     [Google Scholar]
  22. Jones M., King H. K. 1972; Particulate malate oxidation in strictly aerobic bacteria: the respiratory chain of Moraxella Iwoffi. FEBS Utters 22:277–279
     [Google Scholar]
  23. Keevil C. W., Hough J. S., Cole J. A. 1977; The effects of glucose, succinate and 3′,5′-cyclic adenosine monophosphate on the synthesis of the tricarboxylic acid cycle enzymes and respiratory components in Citrobacter freundii. FEMS Micro-biology Letters 1:329–331
     [Google Scholar]
  24. Keevil C. W., Hough J. S., Cole J. A. 1979; Regulation of respiratory and fermentative modes of growth of Citrobacter freundii by oxygen, nitrate and glucose. Journal of General Microbiology 113:83–95
     [Google Scholar]
  25. Meyer D. J., Jones C. W. 1973; Oxidative phosphorylation in bacteria which contain different cytochrome oxidases. European Journal of Biochemistry 36:144–151
     [Google Scholar]
  26. Midgley M., Dawes E. A. 1973; The regulation of transport of glucose and methyl α-glucoside in Pseudomonas aeruginosa. Biochemical Journal 132:141–154
     [Google Scholar]
  27. Milner H. W., Lawrence N. S., French C. S. 1950; Colloidal dispersion of chloroplast material. Science 111:633–634
     [Google Scholar]
  28. Mitchell C. G., Dawes E. A. 1982; The role of oxygen in the regulation of glucose metabolism, transport and the tricarboxylic acid cycle in Pseudomonas aeruginosa. Journal of General Microbiology 128:49–59
     [Google Scholar]
  29. Mitchell P., Moyle J. 1967; Respiration-driven proton translocation in rat liver mitochondria. Biochemical Journal 105:1147–1162
     [Google Scholar]
  30. Nagai S., Nishizawa Y., Aiba S. 1969; Energetics of growth of Azotobacter vinelandii in a glucose-limited chemostat culture. Journal of General Microbiology 59:163–169
     [Google Scholar]
  31. Neijssel O. M., Tempest D. W. 1976; The role of energy-spilling reactions in the growth of Klebsiella aerogenes NCTC418 m aerobic chemostat culture. Archives of Microbiology 110:305–311
     [Google Scholar]
  32. Nishizawa Y., Nagai S., Aiba S. 1971; Effect of dissolved oxygen on electron transport system of Azotobacter vinelandii in glucose-limited and oxygen-limited chemostat cultures. Journal of General and Applied Microbiology 17:131–140
     [Google Scholar]
  33. Pirt S. J. 1975 Principles of Microbe and Cell Cultivation Oxford: Blackwell Scientific Publications;
     [Google Scholar]
  34. Ratcliffe H. D., Drozd J. W., Bull A. T. 1983; The utilization of 5-oxoproline, ammonia and glutamine by Rhizobium leguminosarum in chemostat culture. Journal of General Microbiology 129:1707–1712
     [Google Scholar]
  35. Reynafarje B., Lehninger A. L. 1978; The K+/site and H+/site stoichiometry of mitochondrial electron transport. Journal of Biological Chemistry 253:6331–6334
     [Google Scholar]
  36. Robinson J., Cooper J. M. 1970; Method of determining oxygen concentrations in biological media, suitable for calibration of the oxygen elec-trode. Analytical Biochemistry 33:390–399
     [Google Scholar]
  37. Senior P. J., Beech G. A., Ritchie G. A. F., Dawes E. A. 1972; The role of oxygen limitation in the formation of poly-β-hydroxybutyrate during batch and continuous culture of Azotobacter beijer-inckii. Biochemical Journal 128:1193–1201
     [Google Scholar]
  38. Stouthamer A. H. 1979; The search for correlation between theoretical and experimental growth yields. In International Reviews of Biochemistry. Microbial Biochemistry 211–47 Quayle J. R. Baltimore: University Park Press;
     [Google Scholar]
  39. van Verseveld H. W. 1979; Influence of environmental factors on the efficiency of energy conservation in Paracoccus denitrificans. PhD thesis Vrije Universi-teit te Amsterdam;
     [Google Scholar]
  40. Wales D. S., Cartledge T. G. 1980; Effects of glucose repression and anaerobiosis on the activities and subcellular distribution of tricarboxylic acid cycle and associated enzymes in Saccharomyces carlsbergensis. Journal of General Microbiology 116:93–98
     [Google Scholar]
  41. Ward A. C., Rowley B. I., Dawes E. A. 1977; Effect of oxygen and nitrogen limitation on poly-β-hydroxybutyrate biosynthesis in ammonium grown Azotobacter beijerinckii. Journal of General Microbiology 102:61–68
     [Google Scholar]
  42. Whiting P. H., Midgley M., Dawes E. A. 1976a; The regulation of transport of glucose, gluconate and 2-oxogluconate and of glucose catabo-lism in Pseudomonas aeruginosa. Biochemical Journal 154:659–668
     [Google Scholar]
  43. Whiting P. H., Midgley M., Dawes E. A. 1976b; The role of glucose limitation in the regulation of the transport of glucose, gluconate and 2-oxogluconate, and of glucose metabolism in Pseudomonas aeruginosa. Journal of General Microbiology 92:304–310
     [Google Scholar]
  44. Whittaker P. A. 1971; Terminal respiration in Moraxella Iwoffi. Microbios 4:65–70
     [Google Scholar]
  45. Wimpenny J. W. T. 1969; The effect of E h on regulatory processes in facultative anaerobes. Biotechnology and Bioengineering 11:623–629
     [Google Scholar]
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Effect of Oxygen Concentration on the Growth and Respiratory Efficiency of Acinetobacter calcoaceticus
Microbiology 131, 855 (1985); https://doi.org/10.1099/00221287-131-4-855
/content/journal/micro/10.1099/00221287-131-4-855
/content/journal/micro/10.1099/00221287-131-4-855
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