1887

Abstract

The uptake of succinate and malate has been compared in cultured cells and bacteroids of two species of slow-growing : . (USDA I-110) and cowpea (USDA 3278). Cultured cells of both organisms actively accumulated both compounds, and uptake was abolished by KCN and 2,4-DNP, but not by arsenate. Kinetic studies using cultured cells showed that succinate competitively inhibited malate uptake, and vice versa, implying a common step in the uptake of these dicarboxylic acids. Uptake of both of these compounds was inhibited by osmotic shock and -ethylmaleimide in cultured cells of both species. Purified bacteroids accumulated succinate in a process that was sensitive to 2,4-DNP and KCN, but at a rate significantly slower than for cultured cells. No detectable malate uptake was observed in purified symbiotic cells. Furthermore, succinate uptake was insensitive to osmotic shock in bacteroids of both strains. These results show that although bacteroids of both strains are competent in succinate uptake, significant differences exist in the expression and/or stability of dicarboxylate uptake systems between free-living and symbiotic cells.

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1985-04-01
2024-11-13
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References

  1. American Type Culture Collection 1982; Catalogue of Strains I. , 15.606 Rhizobium X medium Rockville, Md: American Type Culture Collection;
    [Google Scholar]
  2. Anraku Y. 1978; Active transport of amino acids. Bacterial Transport171–219 Rosen B. P. New York: Marcel Dekker;
    [Google Scholar]
  3. Brown C. M., Dilworth M. J. 1975; Ammonia assimilation by Rhizobium cultures and bacteroids. Journal of General Microbiology 86:39–48
    [Google Scholar]
  4. Ching T. M., Hedtke S., Newcomb W. 1977; Isolation of bacteria, transforming bacteria and bacteroids from soybean nodules. Plant Physiology 60:771–774
    [Google Scholar]
  5. Dilworth M. J., Glenn A. R. 1981; Control of carbon substrate utilization by rhizobia. Current Perspectives in Nitrogen Fixation244–251 Gibson A. H., Newton W. E. Amsterdam: Elsevier/North Holland;
    [Google Scholar]
  6. Finan T. M., Wood J. M., Jordan D. C. 1981; Succinate transport in Rhizobium leguminosarum . Journal of Bacteriology 148:193–202
    [Google Scholar]
  7. Finan T. M., Wood J. M., Jordan D. C. 1983; Symbiotic properties of C4-dicarboxylic acid transport mutants of Rhizobium leguminosarum . Journal of Bacteriology 154:1403–1413
    [Google Scholar]
  8. Gardiol A., Arias A., Cervanansky C., Martinez-De Drets G. 1982; Succinate dehydrogenase mutant in Rhizobium meliloti . Journal of Bacteriology 151:1621–1623
    [Google Scholar]
  9. Glenn A. R., Poole P. S., Hudman J. F. 1980; Succinate uptake by free-living and bacteroid forms of Rhizobium leguminosarum . Journal of General Microbiology 119:267–271
    [Google Scholar]
  10. Jordan D. C. 1982; Transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a genus of slow-growing root nodule bacteria from leguminous plants. International Journal of Systematic Bacteriology 32:136–139
    [Google Scholar]
  11. Keele B. B. Jr, Hamilton P. B., Elkan G. H. 1969; Glucose catabolism in Rhizobium japonicum . Journal of Bacteriology 97:1184–1191
    [Google Scholar]
  12. Lo T. C. Y., Rayman M. K., Sanwal B. D. 1972; Transport of succinate in Escherichia coli. I. Biochemical and genetic studies of transport in whole cells. Journal of Biological Chemistry 247:6323–6331
    [Google Scholar]
  13. McAllister C. F., Lepo J. E. 1983; Succinate transport by free-living forms of Rhizobium japonicum . Journal of Bacteriology 153:1155–1162
    [Google Scholar]
  14. Nimmo I. A., Atkins G. L. 1979; The statistical analysis of non-normal (real?) data. Trends in Biochemical Science 4:236–239
    [Google Scholar]
  15. Reibach P. H., Streeter J. G. 1984; Evaluation of active versus passive uptake of metabolites by Rhizobium japonicum bacteroids. Journal of Bacteriology 159:47–52
    [Google Scholar]
  16. Reibach P. H., Mask P. L., Streeter J. G. 1981; A rapid one-step method for the isolation of bacteroids from root nodules of soybean plants, utilizing self-generating Percoli gradients. Canadian Journal of Microbiology 27:491–495
    [Google Scholar]
  17. Riordan J. F., Vallee B. L. 1967; Reactions with N-ethylmaleimide and p-mercuribenzoate. Methods .in Enzymology 11:541–548
    [Google Scholar]
  18. Ronson C. W., Lyttleton P., Robertson J. G. 1981; C4-dicarboxylate transport mutants of Rhizobium trifola form ineffective nodules on Trifolium repens . Proceedings of the National Academy of Sciences of the United States of America 784284–4288
    [Google Scholar]
  19. Sloger C. 1969; Symbiotic effectiveness and N2 fixation in nodulated soybean. Plant Physiology 44:1666–1668
    [Google Scholar]
  20. Ucker D. S., Signer E. R. 1978; Catabolite-repression-like phenomenon in Rhizobium meliloti . Journal of Bacteriology 136:1197–1200
    [Google Scholar]
  21. Verma D. P. S., Kazazian V., Zogbi V., Bal A. K. 1978; Isolation and characterization of the membrane envelope enclosing the bacteroids in soybean root nodules. Journal of Cell Biology 78:919–936
    [Google Scholar]
  22. de Vries G., van Brussel A. A. N., Quispel A. 1982; Mechanism and regulation of glucose transport in Rhizobium leguminosarum . Journal of Bacteriology 149:872–879
    [Google Scholar]
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