1887

Abstract

attached to, penetrated and replicated within the three eukaryotic cell lines, MRC-5, HEp-2 and Vero. Multiplication occurred rapidly in these cells for an initial 48 h after inoculation and declined thereafter. Infected MRC-5 cell monolayers developed lytic-type cytopathic changes, with organisms being readily released. HEp-2 cells showed a more chronic infection, with slowly developing granular changes in the monolayers, and slow release of intracellular bacteria. In Vero cells, organisms were released rapidly along with a more progressively developing granular cytopathic effect in the monolayers. was unable to grow in cell-free culture fluids. Uptake and intracellular development was similar for each cell type, and was initiated by ‘bacteriopexis’, a process in which the organisms bound via receptors and were surrounded by cellular microvilli which eventually fused, leading to bacterial engulfment. Replication of organisms in vacuoles within the cytoplasm of infected cells was confirmed by thorium labelling. These vacuoles were lined with ribosomes and, at the early stages of intracellular development, were found in close proximity to mitochondria, cytoplasmic filaments and banded enclosures. Ruthenium red staining showed that acid mucopolysaccharide capsular material was not present on these organisms during the attachment process or intracellular phase. Organism release was by lysis of the infected cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-4-697
1985-04-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/4/mic-131-4-697.html?itemId=/content/journal/micro/10.1099/00221287-131-4-697&mimeType=html&fmt=ahah

References

  1. Chandler F. W., Cole R. M., Hicklin M. D., Blackmon J. A., Callaway C. S. 1979; Ultra-structure of the Legionnaires’ disease bacterium. A study using transmission electron microscopy. Annals of Internal Medicine 90:642–647
    [Google Scholar]
  2. Daisy J. A., Benson C. E., McKitrick J., Friedman H. M. 1981; Intracellular replication of Legionella pneumophila . Journal of Infectious Diseases 143:460–464
    [Google Scholar]
  3. Davis G. S., Winn W. C., Gump D. W., Craighead J. E., Beaty H. N. 1982; Legionnaires’ pneumonia after aerosol exposure in guinea-pigs and rats. American Review of Respiratory Disease 126:1050–1057
    [Google Scholar]
  4. Dennis P. J., Taylor J. A., Barrow G. I. 1981; Phosphate buffered, low sodium chloride blood agar medium for Legionella pneumophila . Lancet 2:656
    [Google Scholar]
  5. Edelstein P. H. 1984; Laboratory diagnosis of Legionnaires’ disease. Legionella3–5 Thornsberry C., Balows A., Feeley J. C., Jakubowski W. Washington: ASM;
    [Google Scholar]
  6. Flesher A. R., Ito S., Mansheim B. J., Kasper D. L. 1979; The cell envelope of the Legionnaires’ disease bacterium. Morphological and biochemical characteristics. Annals of Internal Medicine 90:628–630
    [Google Scholar]
  7. Friedman R. L., Lochner J. E., Bigley R. H., Iglewski B. H. 1982; The effects of Legionella pneumophila toxin on oxidative processes and bacterial killing of human poly-morphonuclear leucocytes. Journal of Infectious Diseases 146:328–334
    [Google Scholar]
  8. Glavin F. L., Winn W. C., Craighead J. E. 1979; Ultrastructure of lung in Legionnaires’ disease, observations on three biopsies done during the Vermont epidemic. Annals of Internal Medicine 90:555–559
    [Google Scholar]
  9. Horwitz M. A. 1984; Interactions between Legionella pneumophila and human mononuclear phagocytes. In Legionella159–166 Thornsberry C., Balows A., Feeley J. C., Jakubowski W. Washington: A.S.M.;
    [Google Scholar]
  10. Horwitz M. A., Silverstein S. C. 1980; The Legionnaires’ disease bacterium (Legionella pneumophila) multiplies intracellularly in human monocytes. Journal of Clinical Investigation 66:441–450
    [Google Scholar]
  11. Horwitz M. A., Silverstein S. C. 1981; Interaction of the Legionnaires’ disease bacterium (Legionella pneumophila) with human phagocytes 1: L. pneumophila resists killing by polymorphonuclear leukocytes, antibody and complement. Journal of Experimental Medicine 153:386–397
    [Google Scholar]
  12. Kishimoto R. A., Kastello M. D., White J. D., Shirey F. G., McGann V. G., Larson E. W., Hedlund K. W. 1979; In υitro interaction between normal cynolmolgus monkey alveolar macrophages and Legionnaires’ disease bacteria. Injection and Immunity 25:761–763
    [Google Scholar]
  13. Kishimoto R. A., White J. D., Shirey F. G., McGann V. G., Berendt R. F., Larson E. W., Hedlund K. W. 1981; In υitro response of guineapig peritoneal macrophages to Legionella pneumophila . Infection and Immunity 31:1209–1213
    [Google Scholar]
  14. Rodgers F. G. 1979; Ultrastructure of Legionella pneumophila . Journal of Clinical Pathology 32:1195–1202
    [Google Scholar]
  15. Rodgers F. G. 1983; The role of structure and invasiveness on the pathogenicity of Legionella . Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (Abteilung I, Originale A) 255:138–144
    [Google Scholar]
  16. Rodgers F. G., Davey M. R. 1982; Ultrastructure of the cell envelope layers and surface details of Legionella pneumophila . Journal of General Microbiology 128:1547–1557
    [Google Scholar]
  17. Rodgers F. G., Macrae A. D., Lewis M. J. 1978; Electron microscopy of the organism of Legionnaires’ disease. Nature London: 272825–826
    [Google Scholar]
  18. Rodgers F. G., Greaves P. W., Macrae A. D., Lewis M. J. 1980; Electron microscopic evidence of flagella and pili on Legionella pneumophila . Journal of Clinical Pathology 33:1184–1188
    [Google Scholar]
  19. Winn W. C., Davis G. S., Gump D. W., Craighead J. E., Beaty H. N. 1982; Legionnaires’ pneumonia after intratracheal inoculation of guinea-pigs and rats. Laboratory Investigation 47:568–578
    [Google Scholar]
  20. Wong M. C., Ewing E. P., Callaway C. S., Peacock W. L. 1980; Intracellular multiplication of Legionella pneumophila in cultured human embryonic lung fibroblasts. Infection and Immunity 28:1014–1018
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-4-697
Loading
/content/journal/micro/10.1099/00221287-131-4-697
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error