1887

Abstract

Partial inhibition of lipid synthesis in by the inclusion of cerulenin in growth media led to the formation of chains of spheroidal cells both of wild-type strains and mutants. Washed cell suspensions prepared from wild-type cultures treated with cerulenin lysed only very slowly compared with those from control cultures although the activity of autolysins in 5 -LiCl extracts made from such organisms was only about 30% less than those in extracts from controls. The behaviour of the cultures, such as the separation of cells and the reaction to -lactams, was as if they were grossly deficient in autolytic activity. The concentration of cerulenin used (7·5 μg ml) reduced the growth rate two- to threefold but exponential growth at this slower rate continued for at least 18 h. The steady state concentration of total protein and peptidoglycan per unit bacterial mass was the same as in control cultures but the phospholipid content was reduced by 50%.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-3-591
1985-03-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/3/mic-131-3-591.html?itemId=/content/journal/micro/10.1099/00221287-131-3-591&mimeType=html&fmt=ahah

References

  1. Berkeley R. C. W., Pepper E. A., Caulfield M. P., Melling J. 1978; Inhibition of enterotoxin A production by cerulenin and quina-crine. Presumptive evidence for a lipid intermediate protease release mechanism. FEMS Letters 4:103–107
    [Google Scholar]
  2. Brogue R. M., Niederman R. A. 1979; Membranes of Rhodopseudomonas spheroides: effect of cerulenin on assembly of chromatophore membrane. Journal of Bacteriology 138:788–798
    [Google Scholar]
  3. Carson D. D., Pieringer R. A., Daneo-Moore L. 1981; Effect of cerulenin on cellular autolytic and lipid metabolism during inhibition of protein synthesis in Streptococcus faecalis. Journal of Bacteriology 146:590–604
    [Google Scholar]
  4. Caulfield M. P., Berkeley R. C. W., Pepper E. A., Melling J. 1979; Export of extracellular levan sucrase by Bacillus subtilis: inhibition by cerulenin and quinacrine. Journal of Bacteriology 138:345–351
    [Google Scholar]
  5. Fein J. E., Rogers H. J. 1976; Autolytic enzyme-deficient mutants of Bacillus subtilis 168. Journal of Bacteriology 127:1427–1442
    [Google Scholar]
  6. Fishman Y., Rotham S., Citri N. 1980; Preferential suppression of normal exo-enzyme formation by membrane-modifying agents. Journal of Bacteriology 141:1435
    [Google Scholar]
  7. Higgins M. L., Carson D. D., Daneo-Moore L. 1980; Morphological effect of cerulenin treatment on Streptococcus faecalis as studied by ultrastructure reconstruction. Journal of Bacteriology 143:989–994
    [Google Scholar]
  8. Ito E. T., Cihlar R. L., Inderlied C. B. 1982; Lipid synthesis during morphogenesis of Mucor racemosus. Journal of Bacteriology 152:880–887
    [Google Scholar]
  9. Jacques N. A. 1983; Membrane perturbation by cerulenin modulates glucosyltransferase secretion and acetate uptake by Streptococcus salivarius. Journal of General Microbiology 129:3293–3302
    [Google Scholar]
  10. Janczura E., Perkins H. R., Rogers H. J. 1961; Teichuronic acid: a mucopolysaccharide in wall preparations from vegetative cells of Bacillus subtilis. Biochemical Journal 80:82–93
    [Google Scholar]
  11. Leduc M., Kasra R., Van Heijenoort J. 1982; Induction and control of the autolytic system of Escherichia coli. Journal of Bacteriology 152:26–34
    [Google Scholar]
  12. Leung W.-L., Harlander S. K., Schachtell F. 1980; Streptococcus mutans dextransucrase: effect of cerulenin on lipid synthesis and enzyme production. Infection and Immunity 28:846–852
    [Google Scholar]
  13. Omura S. 1976; The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis. Bacteriological Reviews 40:681–697
    [Google Scholar]
  14. Paton J. C, May B. K., Elliot W. H. 1980; Cerulenin inhibits production of extracellular proteins but not membrane proteins in Bacillus amylo-liquefaciens. Journal of General Microbiology 118:178–187
    [Google Scholar]
  15. Petit-Glatron M. F., Chambert R. 1981; Levan-sucrase of Bacillus subtilis. Conclusive proof that production and export are unrelated to fatty acid synthesis but modulated by membrane modifying agents. European Journal of Biochemistry 119:603–611
    [Google Scholar]
  16. Rogers H. J. 1985; Morphological changes in Bacillus subtilis with genetic and phenotypic origins. Annales de Microbiologie in the Press
    [Google Scholar]
  17. Rogers H. J., Taylor C. 1978; Autolysins and shape change in rodA mutants of Bacillus subtilis. Journal of Bacteriology 135:1032–1042
    [Google Scholar]
  18. Rogers H. J., Thurman P. F. 1978; Temperature-sensitive nature of the rodB mutation in Bacillus subtilis. Journal of Bacteriology 133:298–305
    [Google Scholar]
  19. Rogers H. J., Thurman P. F., Burdett I. D. J. 1983; The bactericidal action of β-lactam antibiotics on an autolysin-deficient strain of Bacillus subtilis. Journal of General Microbiology 129:465–478
    [Google Scholar]
  20. Rogers H. J., Taylor C, Rayter S., Ward J. B. 1984; Purification and properties of autolytic endo-β-N-acetylglucosaminidase and the N-acetyl-muramyl-l-alanine amidase from Bacillus subtilis strain 168. Journal of General Microbiology 130:2395–2402
    [Google Scholar]
  21. Vance D. I., Goldberg I., Mitsuhashi O., Block K., Omura S., Momura S. 1972; Inhibition of fatty acid synthetase by the antibiotic cerulenin.. Biochemical and Biophysical Research Communications 48:649–656
    [Google Scholar]
  22. Willie W. E., Eisenstadt E., Willecke K. 1975; Inhibition of de novo fatty acid synthesis by the antibiotic cerulenin in Bacillus subtilis: effects on citrate-Mg2+ transport and synthesis of macromole-cules. Antimicrobial Agents and Chemotherapy 8:231–237
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-3-591
Loading
/content/journal/micro/10.1099/00221287-131-3-591
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error