Summary: Esterases, and glutamate, lactate and malate dehydrogenases of 64 and strains, were analysed by polyacrylamide agarose gel electrophoresis and by thin layer isoelectrofocusing. On the basis of the isoelectric points of malate dehydrogenase from the three species and the mobility of lactate dehydrogenase from 8 species specific zymotypes were defined: three for strains, three for strains and two for strains. These zymotypes correlated with previously established DNA hybridization groups. The other electrophoretic data were found to be less useful for distinction between and strains, but supported differentiation into zymotypes for strains. The two-dimensional electrophoretic profile established by plotting isoelectric point against electrophoretic mobility of the major esterase illustrated the degree of enzyme polymorphism among the strains of the three species. Variation in electrophoretic patterns within and might provide useful epidemiological markers.


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