1887

Abstract

Summary: Lipopolysaccharides from phase I (LPSI) Ohio and Nine Mile strains and from phase II (LPSII) Nine Mile strain were stained negatively and positively and examined with the electron microscope. The ultrastructure of LPSI and LPSII positively stained with uranyl formate or uranyl acetate was ribbon-like. When negatively stained with uranyl acetate, LPSI was ribbon-like but LPSII exhibited hexagonal lattice structures. However, LPSII stained negatively with sodium phosphotungstate and ammonium molybdate exhibited hexagonal lattice ultrastructures which were not identical to those observed when negatively stained with uranyl acetate. The hexagonal lattice structures formed were due to the interactions of LPSII and the staining reagents rather than to protein-LPS interactions. The differences in the ultrastructures of LPSI and LPSII are undoubtedly based on variations in their chemical composition.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-11-3127
1985-11-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/11/mic-131-11-3127.html?itemId=/content/journal/micro/10.1099/00221287-131-11-3127&mimeType=html&fmt=ahah

References

  1. Acker G., Wartenberg K. 1976; Ultrastructure of lipopolysaccharides of Yersinia enterocolitica, Salmonella typhimurium and Escherichia coli. Zen-tralblatt Jur Bakteriologie, Mikrobiologie und Hygiene (Abteilung I, Originate A) 235:439–452
    [Google Scholar]
  2. Amano K., Fukushi K. 1984a; Chemical and ultrastructural differences in endotoxic glycolipids from Salmonella typhimurium R mutant extracted with various solvent systems. Microbiology and Immunology 28:135–452
    [Google Scholar]
  3. Amano K., Fukushi K. 1984b; Chemical and ultrastructural comparison of endotoxins extracted from Salmonella minnesota wild type and R mutants. Microbiology and Immunology 28:149–159
    [Google Scholar]
  4. Amano K., Fukushi K. 1984c; Electron microscopic studies of endotoxins treated with alkaline and acid reagents. Microbiology and Immunology 28:161–168
    [Google Scholar]
  5. Amano K., Williams J. C. 1984; Chemical and immunological characterization of lipopolysaccharides from phase I and phase II Coxiella burnetii. Journal of Bacteriology 160:994–1002
    [Google Scholar]
  6. Baca O. G., Martinez I. L., Aragon A. S., Klassen D. 1980; Isolation and partial characterization of a lipopolysaccharide from phase II Coxiella burnetii. Canadian Journal of Microbiology 26:819–826
    [Google Scholar]
  7. Chan M. L., Mcchesney J., Paretsky D. 1976; Further characterization of a lipopolysaccharide from Coxiella burnetii. Infection and Immunity 13:1721–1727
    [Google Scholar]
  8. Kato N., Ohta M., Kido N., Ito H., Naito S., Kuno T. 1985; Formation of a hexagonal lattice structure by an R-form lipopolysaccharide of Klebsiella sp. Journal of Bacteriology 162:1142–1150
    [Google Scholar]
  9. Nikaido H. 1979; Nonspecific transport through the outer membrane In Bacterial Outer Membranes. Biogenesis and Functions pp 361–407 Edited by Inouye M. New York: John Wiley;
    [Google Scholar]
  10. Schramek S., Mayer H. 1982; Different sugar compositions of lipopolysaccharides isolated from phase I and phase II cells of Coxiella burnetii. Infection and Immunity 38:53–57
    [Google Scholar]
  11. Schramek S., Radziejewska-Lebrecht J., Mayer H. 1985; 3-C-Branched aldoses in lipopolysaccharide of phase I Coxiella burnetii and their role as immunodominant factors. Eropean Journal of Biochemistry 148:455–461
    [Google Scholar]
  12. Shands J. W. JR 1971; Evidence for bilayer structure in gram-negative lipopolysaccharide: relationship to toxicity. Infection and Immunity 4:167–172
    [Google Scholar]
  13. Shands J. W. JR, Graham J. A., Nath K. 1967; The morphologic structure of isolated bacterial lipopolysaccharide. Journal of Molecular Biology 25:15–21
    [Google Scholar]
  14. Williams J. C., Peacock M. G., Mccaul T. F. 1981; Immunological and biological characterization of Coxiella burnetii, phases I and II, separated from host components. Infection and Immunity 32:840–851
    [Google Scholar]
  15. Yamada H., Mizushima S. 1980; Interaction between major outer membrane protein (O-8) and lipopolysaccharide in Escherichia coli K12. European Journal of Biochemistry 103:209–218
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-11-3127
Loading
/content/journal/micro/10.1099/00221287-131-11-3127
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error