Summary: Colloidal gold conjugated goat anti-rabbit IgG and specific antisera raised against purified thioredoxins from and have been used to determine the localization of thioredoxin in whole filaments of and Vegetative cells of were labelled differently when tested with either of the two anti-thioredoxin sera. The anti- thioredoxin serum reacted only with thioredoxin which was located predominantly in the nucleoplasm in vegetative cells; it showed no association with the chromatoplasm. Anti- thioredoxin serum labelled vegetative cells of , particularly in the vicinity of the thylakoid membranes and also labelled in the vicinity of the thylakoid membranes, indicating that a second thioredoxin or thioredoxin-like protein was present in this cyanobacterium. Heterocysts of and showed substantially less labelling than vegetative cells when tested with both antisera.


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