1887

Abstract

Summary: Bacteriophage Mu pf7701, a Km derivative of Mu ts62, was inserted into the conjugative plasmid pDC251, which carries Tn. When pDC251 : : Mu pf7701 plasmids were conjugated from into derivatives of strain SS104 they were unstable; loss of either or both drug resistance markers occurred. Stable transconjugants resulted from deletion of Mu sequences, integration of the plasmid into the chromosome, or loss of an indigenous 34 kb cryptic plasmid. Among transconjugants selected for Km, the largest colonies arose from transconjugants in which Mu pf7701 had transposed to the chromosome and the pDC251 : : Mu pf7701 plasmid had been lost; 1300 transconjugants of this type were screened for pathogenicity to corn () seedlings and eight mutants were obtained that did not cause watersoaking symptoms. The insertions of Mu pf7701 in these mutants were in the chromosome.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-11-2993
1985-11-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/11/mic-131-11-2993.html?itemId=/content/journal/micro/10.1099/00221287-131-11-2993&mimeType=html&fmt=ahah

References

  1. Beringer J. E., Beynon J. L., Buchanan-Wollas-Ton A. V., Johnston A. W. B. 1978; Transfer of the drug resistance transposon Tn5to Rhizobium. Nature, London 276:633–634
    [Google Scholar]
  2. Birnboim H. A., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Bradshaw-Rouse J. J., Whatley M. A., Coplin D. L., Woods A., Sequeira L., Kelman A. 1981; Agglutination of strains of Erwinia stewartiiwith a corn agglutinin: correlation with extracellular poly-saccharide production and pathogenicity. Applied and Environmental Microbiology 42:344–350
    [Google Scholar]
  4. Bukhari A. I., Taylor A. L. 1975; Influence of insertions on packaging of host sequences covalently linked to bacteriophage Mu DNA. Proceedings of the National Academy of Sciences of the United States of America 72:4399–4403
    [Google Scholar]
  5. Casey C., Bolton E., O’Gara F. 1983; Behavior of bacteriophage Mu-based IncP suicide vector plasmids in Rhizohiumspp. FEMS Microbiology Letters 20:217–223
    [Google Scholar]
  6. Chatterjee A. K., Brown M. A., Ziegle J. S. 1981; Isolation and characterization of a temperature-sensitive (ts) mutant of a broad-host-range IncPl R plasmid pUT13. Phytopathology 71:866
    [Google Scholar]
  7. Chatterjee A. K., Thurn K. K., Feese D. A. 1983; Tn5-induced mutations in the enterobacterial phytopathogen Erwinia chrysanthemi. Applied and Environmental Microbiology 45:644–650
    [Google Scholar]
  8. Chow L. T., Kahmann R., Kamp D. 1977; Electron microscopic characterization of DNAs of nondefective deletion mutants of bacteriophage Mu. Journal of Molecular Biology 113:591–609
    [Google Scholar]
  9. Coplin D. L. 1978; Properties of F and P group plasmids in Erwinia stewartii. Phytopathology 68:1637–1643
    [Google Scholar]
  10. Coplin D. L. 1979; Introduction of bacteriophage Mu into Erwinia stewartiiby use of an RK2: :Mu hybrid plasmid. Journal of General Microbiology 113:181–184
    [Google Scholar]
  11. Coplin D. L., Rowan R. G. 1979; Conjugative plasmids in Erwinia stewartii.. In Proceedings of the 4th International Conference on Plant Pathogenic Bacteria pp. 67–73 Angers, France: Station de Pathologie Végétale et Phytobactériologie, Institut National de la Recherche Agronomique;
    [Google Scholar]
  12. Coplin D. L., Rowan R. G., Chisholm D. A., Whitmoyer R. E. 1981; Characterization of plasmids in Erwinia stewartii. Applied and Environmental Microbiology 42:599–604
    [Google Scholar]
  13. Coplin D. F., Frederick R. D., McCammon S. L. 1985; Characterization of a conjugative plasmid from Erwinia stewartii. Journal of General Micro-biology 131:2985–2991
    [Google Scholar]
  14. Currier T. C., Nester E. W. 1976; Isolation of covalently closed circular DNA of high molecular weight from bacteria. Analytical Biochemistry 76:431–441
    [Google Scholar]
  15. Gantotti B. V., Kindle K. K., Beer S. V. 1981; Transfer of the drug-resistance transposon Tn5to Erwinia herbicolaand the induction of insertion mutations. Current Microbiology 6:377–381
    [Google Scholar]
  16. Hoopes B. C., Mcclure W. R. 1981; Studies on the selectivity of DNA precipitation by spermine. Nucleic Acids Research 9:5493–5504
    [Google Scholar]
  17. Howe M. M. 1973; Prophage deletion mapping of bacteriophage Mu-1. Virology 54:93–101
    [Google Scholar]
  18. Schumann W. 1979; Cloning and biological characterization of the immunity region of Escherichia coliphage Mu. Gene 5:275–290
    [Google Scholar]
  19. Thierry A., Chambost J.-P., Kotoujansky A., Cattaneo J., Bertheau Y., Barras F., Van Gijsegm F., Coleno A. 1984; Mutants of Erwinia chrysanthemidefective in secretion of pectin-ase and cellulase. Journal of Bacteriology 160:1199–1203
    [Google Scholar]
  20. Thompson C. J., Howe M. 1979; Tn5induced mutations in bacteriophage Mu. In Abstracts of the Annual Meeting of the American Society of Microbiology, abstract no. S44 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  21. Van Gijsegm F., Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. 1985; Isolation and characterization of Erwinia chrysanthemimutants defective in degradation of hexuronates. Journal of Bacteri-ology 161:702–708
    [Google Scholar]
  22. Waggoner B. T., Marrs C. F., Howe M. M., Pato M. C. 1984; Multiple factors and processes involved in host cell killing by bacteriophage Mu: characterization and mapping. Virology 136:168–185
    [Google Scholar]
  23. Zink R. T., Kemble R. J., Chatterjee A. K. 1984; Transposon Tn5mutagenesis in Erwinia carotovorasubsp. carotovoraand E. carotovorasubsp. atroseptica. Journal of Bacteriology 157:809–814
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-11-2993
Loading
/content/journal/micro/10.1099/00221287-131-11-2993
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error