1887

Abstract

Summary: Pseudomonas aeruginosa strain PAOl was compared to PAOl strains containing an IncP-l R plasmid (RPl. R68, or R68.45) in an experimental mouse burn infection model. All R plasmids tested caused a 10-to 400-fold increase in mean lethal dose (LD). The decrease in virulence produced by plasmids R68 and R68.45 was significantly greater than the decrease caused by the closely related plasmid RPl. All plasmids also led to an increased sensitivity of strain PAOl to human serum bactericidal activity. Virulence and serum resistance of strain PAOl were restored by curing of the entire plasmid R68.45 but not by deletions in the plasmid’s transfer gene regions.

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1985-10-01
2021-07-29
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References

  1. Barth P. T. 1979; RP4 and R300B as wide host-range plasmid cloning vehicles. In Plasmids of Medical, Entironmental and Commerical Importance pp. 399–410 Edited by Timmis K. N., Pühler A. Amsterdam: ElsevieriNorth-Holland:
    [Google Scholar]
  2. Binns M. M., Mayden J., Levine R. B. 1982; Further characterization of complement resistance conferred on Escherichia coli by the plasmid genes traT of RIOO and iss of ColV, 1-K94. hifection and lmmunitr 34:654–659
    [Google Scholar]
  3. Burkardt H.-J., Riess G., PÜHler A. 1979; Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical. Journal of General Microbiology 114:341–348
    [Google Scholar]
  4. Caruso M., Shapiro J. A. 1982; Interactions of Tn7 and temperate phage F1 16L of Pseudomonas aeruginosa. Molecular and General Genetics 188:292–298
    [Google Scholar]
  5. Clark R. B., Janda J. M., Bottone E. J. 1984; Phenotypic properties correlated with the absence of virulence among gentamicin-resistant Pseudomonas aeruginosa strains. Journal ol Clinical Microbiology 20:235–238
    [Google Scholar]
  6. Currier T. C., Morgan M. K. 1981; Restriction endonuclease analysis of the incompatibility group P-1 plasmids RK2, RP1, RP4. R68 and R68.45. Current Microbiology 5:323–327
    [Google Scholar]
  7. Daum R. S., Syriopoulou V. P., Smith A. L., Scheifele D. w., Willard J. E. 1981; Loss of plasmid DNA coding for β-lactamase during experimental infection with Haemophilus influenzae type b. Journal of Infectious Diseases 143:548–553
    [Google Scholar]
  8. Elwell L. P., Shipley P. L. 1980; Plasmidmediated factors associated with virulence of bacteria to animals. Annual Review of Microbiology 34:465–496
    [Google Scholar]
  9. Finney D. J. 1964; The Spearman-Kärber method. In Statistical Methods in Biological Assays, 2nd. edn pp. 524–530 London: Charles Griffin;
    [Google Scholar]
  10. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. 1972; Properties of an R factor which originated in Pseudomonas aeruginosa 1822. Journal of Bacteriology 110:529–537
    [Google Scholar]
  11. Grinter N. J. 1985; Plasmid stability: involvement of a plasmid-coded primase and site-specific recombination/resolution systems. In Plasmids in Bacteria p. 858 Edited by Helinski D., Cohen S. N., Clewell D. Z., Jackson D., Hollaender A. New York: Plenum Press;
    [Google Scholar]
  12. Haas D., Holloway B. w. 1976; R factor variants with enhanced sex factor activity in Pseudommws aeruginosa. Molecular anti General Genetics 144:243–251
    [Google Scholar]
  13. Haas D., Riess G. 1983; Spontaneous deletions of the chromosome-mobilizing plasmid R68.45 in Pseudomonas aeruginosa PAO. Plasmid 09:42–52
    [Google Scholar]
  14. Khakoo R. A., Kluge R. M. 1978; Decreased virulence of gentamicin-resistant strains of Pseudomonas aeruginosa in a rat model. Journal of Laboratory and Clinical Medicine 91:96–103
    [Google Scholar]
  15. Klemperer R. M. M., Ismail N. T. A. J., Brown M. R. W. 1979; Effect of R plasmid RPl on the nutritional requirements of Escherichia coli in batch culture. Journal of General Microbiology 115:325–331
    [Google Scholar]
  16. Lacey R. W. 1975; A critical appraisal of the importance of R-factors in the Enterobacteriaceae in riro. Journal Antimicrobial Chemotherapy 01:25–37
    [Google Scholar]
  17. Liu P. V. 1973; Exotoxins of Pseudomonas aeruginosa. I. Factors that influence the production of exotoxin A. Journal of Infectious Diseases 128:506–513
    [Google Scholar]
  18. Lowbury E. J. L., Kidson A., Lilly H. A., Ayliffe G. A. J., Jones R. J. 1969; Sensitivity of Pseudomonas aeruginosa to antibiotics: emergence of strains highly resistant to carbenicillin. lancet ii:448–452
    [Google Scholar]
  19. Mcmanus A. T., Northam W. J., Moody E. E. M. 1981; Suppression of Pseudomonas aeruginosa burn surface infection by plasmid RPl. Abstracts of the Annual Meeting of the American Society.for Microbiology Abstract B15
    [Google Scholar]
  20. Pavlovskis O. R., Wretlind B. 1979; Assessment of protease (elastase) as a Pseudomonas aeruginosa virulence factor in experimental mouse burn infection. Infection and Immunity 24:181–187
    [Google Scholar]
  21. Pavlovskis O. R., Yaffe L. J., Wretlind B., Galloway D. R. 1983; Experimental Pseudomonas aeruginosa infections in burned mice and rats: description of a model. In Experimental Bacterial and Parasitic Infections pp. 103–108 Edited by Keusch G., Wadstrom T. New York:: Elsevier;
    [Google Scholar]
  22. Pemberton J. M., Holloway B. W. 1973; A new sex factor of Pseudomonas aeruginosa. Genetical Research 21:263–272
    [Google Scholar]
  23. Smith H. R., Scotland S. M., Rowe B. 1983; Plasmids that code for production of colonization factor antigen II and enterotoxin production in strains of Escherichia coli. Infection and Immunity 40:1236–1239
    [Google Scholar]
  24. Stanisich V. A., Holloway B. W. 1971; Chromosome transfer in Pseudomonas aeruginosa mediated by R factors. Genetical Research 17:169–172
    [Google Scholar]
  25. Stieritz D. D., Holoer I. A. 1975; Experimental studies of the pathogenesis of infections due to Pseudomonas aeruginosa: description of a burned mouse model. Journal oflnjectious Diseases 131:688–691
    [Google Scholar]
  26. Thomassen M. J., Demko C. A. 1981; Serum bactericidal effect on Pseudomonas aeruginosa isolates from cystic fibrosis patients. lnfection and Immunity 33:512–518
    [Google Scholar]
  27. Willetts N. S., Crowther C., Holloway B. W. 1981; The insertion sequence IS21 of R68.45 and the molecular basis for mobilization of the bacterial chromosome. Plasmid 6:30–52
    [Google Scholar]
  28. Williams P. H. 1979; Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli. Infection and Immunity 26:925–932
    [Google Scholar]
  29. Wright B. L., Carrick L. JR, Jollick J. D. 1977; The effect of an R-factor on the virulence of Pseudomonas aeruginosa in the mouse. Abstracts of the Annual Meeting of the American Society for Microbiology Abstract Bl 17
    [Google Scholar]
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