1887

Abstract

Summary: Four IncT plasmids were compared for various chatacters, in particular pilus synthesis and function at different temperatures. The prototype Rtsl differed in some respects from the others (R402, R394, pIN25). At 37 °C, the supposedly temperature-sensitive conjugation systems of the plasmids could still function efficiently on a surface, but not in a liquid. Long conjugative pili were synthesized at 30 °C, but only short ones (approx. 200 nm) were produced at 37 °C. The long pili converted two surface-obligatory conjugation systems to surface + liquid ones at 30 °C.

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/content/journal/micro/10.1099/00221287-131-10-2665
1985-10-01
2022-01-17
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References

  1. Bradley D. E. 1967; Ultrastructure of bacteriophages and bacteriocins. Bacteriological Reviews 31:230–314
    [Google Scholar]
  2. Bradley D. E. 1980; Morphological and serological relationships of conjugative pili. Plusmid 4:155–169
    [Google Scholar]
  3. Bradley D. E. 1984; Characteristics and function of thick and thin conjugative pili determined by transfer-derepressed plasmids of incompatibility groups I1, I2, I5, B, K and Z. Journal of General Microbiology 130:1489–1502
    [Google Scholar]
  4. Bradley D. E., Fleming J. 1983; Incompatibility group identification for repressed plasmids using host cell lysis by specific bacteriophages. Journal of Microbiological Methods 1:171–176
    [Google Scholar]
  5. Bradley D. E., Taylor D. E., Cohen D. R. 1980; Specification of surface mating systems among conjugative drug resistance plasmids in Escherichiu coli K-12. Journal of Bacteriology 143:1466–1470
    [Google Scholar]
  6. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981; Phage t : a group T plasmid dependent bacteriophage. Journal of General Microbiology 126:397–403
    [Google Scholar]
  7. Coetzee J. N., Datta N., Hedges R. W. 1972; R factors from Proteus rettgeri. Journal of General Microbiology 72:543–552
    [Google Scholar]
  8. Coetzee J. N., Bradley D. E., Fleming J., Dutoit L., Hughes V. M., Hedges R. W. 1985; Phage pilHα: a phage which adsorbs to IncHI and IncHII plasmid-coded pili. Journul of General Microbiology 131:1115–1121
    [Google Scholar]
  9. Datta N. 1977; Classification of plasmids as an aid to understanding their epidemiology and evolution. Journal of Antimicrobial Chemotherapr 3:suppl. C19–23
    [Google Scholar]
  10. Ishihara M., Kamio Y., Terawaki Y. 1978; Cupric ion resistance as a new genetic marker of a temperature-sensitive R plasmid Rtsl in Escherichia coli. Biochemicul and Biophysical Research Communications 8274–80
    [Google Scholar]
  11. Jacob A. E., Shapiro J. A., Yamamoto L., Smith D. I., Cohen S. N., Berg D. 1977; Plasmids studied in Escherichia coli and other enteric bacteria. In DNA Insertion Elements, Plasmids and tpisomes607–638 Bukhari A. I., Shapiro J. A., Adhya S. L. New York: Cold Spring Harhor Laboratory;
    [Google Scholar]
  12. Kado C. I., Liu S.-T. 1981; Rapid procedure for the detection and isolation of large and small plasmids. Journal of Bacteriology 145:1365–1373
    [Google Scholar]
  13. Levy S. B., Hedges R. W., Sullivan F., Medeiros A. A., Sosrosputro H. 1985; Multiple antibiotic resistance plasmids in Enterobucteriaceae isolated from diarrhoeal specimens of hospitalized children in Indonesia. Journal qi Antimicrobial Chemotherapy 16:in the Press
    [Google Scholar]
  14. Matthew M., Hedges R. W. 1976; Analytical isoelectric focussing of R-factor determined β-lactamases: correlation with plasmid compatibility. Journal of Bacteriology 125:713–718
    [Google Scholar]
  15. Odakura Y., Hashimoto H., Mitsuhashi S. 1977; Temperature sensitive R plasmids isolated from Proteus strains. Microhiology and Immunology 21621–629
    [Google Scholar]
  16. Taylor D. E. 1983; Transfer-defective and tetracy cline-sensitive mutants of the incompatibility group H 1 plasmid R27 generated by insertion of transpo son 7. Plasmid 9:227–239
    [Google Scholar]
  17. Taylor D. E., Levine J. G. 1980; Studies of the temperature-sensitive transfer and maintenance of H incompatibility group plasmids. Journal ofGeneral Microbiology 116:475–484
    [Google Scholar]
  18. Terawaki Y., Rownd R. 1972; Replication of the R factor RtsI in Proteus mirabilis. Journal of Bacteriology 109:492–498
    [Google Scholar]
  19. Terawaki Y., Takayasu H., Akiba T. 1967; Thermosensitive replication of a kanamycin resis tance factor. Journal of Bacteriology 94687–690
    [Google Scholar]
  20. Tetaz T. J., Luke R. K. 1983; Plasmid-controlled resistance to copper in Escherichia coli. Journal of Bacteriology 154:1263–1268
    [Google Scholar]
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