1887

Abstract

Summary: A proline-producing strain of grew more rapidly than the wild-type strain in a medium of high osmolarity due to high concentrations of NaCl, KCl, NaSO, (NH) HPO, sodium glutamate, glucose or sucrose. Growth inhibition by NaCl was partially reversed by proline in the wild-type strain, and by glutamate and proline in the proline-producing strain. Intracellular proline and glutamate concentrations under conditions of high osmolarity were studied. The wild-type strain accumulated endogenously synthesized glutamate, and concentrated proline taken up from the external medium. In contrast, the proline-producing strain accumulated a large amount of endogenously synthesized proline. This increased proline content contributes to the osmotolerance of the proline-producing strain. The growth inhibition by NaCl was also reversed by glycinebetaine in . wild-type and proline-producing strains.

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/content/journal/micro/10.1099/00221287-131-10-2515
1985-10-01
2021-10-21
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References

  1. Adams E., Frank L. 1980; Metabolism of proline and hydroxyproline. Annual Review of Biochemistry 49:1005–1061
    [Google Scholar]
  2. Brown A. D. 1974; Microbial water relations: features of the intracellular composition of sugar-tolerant yeasts. Journal of Bacteriology 118:769–777
    [Google Scholar]
  3. Brown C. M., Stanley S. O. 1972; Environment-mediated changes in the cellular content of the ‘pool’ constituents and their associated changes in cell physiology. Journal of Applied Chemistry and Biotechnology 22:363–389
    [Google Scholar]
  4. Chibata I., Itoh H., Morimoto T. 1977; Automated recording incubator for tube culture of microorganisms. Chemical Economy and Engineering Review 9:11–15
    [Google Scholar]
  5. Csonka L. N. 1981; Proline over-production results in enhanced osmotolerance in Salmonella typhimurium. Molecular and General Genetics 182:82–86
    [Google Scholar]
  6. Csonka L. N. 1982; A third L-proline permease in Salmonella typhimurium which functions in media of elevated osmotic strength. Journal of Bacteriology 151:1433–1443
    [Google Scholar]
  7. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B12. Journal of Bacteriology 60:17–28
    [Google Scholar]
  8. Dendinger S. H., Patil L G., Brenchley J. E. 1980; Salmonella typhimurium mutants with altered glutamate dehydrogenase and glutamate synthase activities. Journal of Bacteriology 141:190–198
    [Google Scholar]
  9. Lerudulier D., Valentine R. C. 1982; Genetic engineering in agriculture: osmoregulation. Trends in Biochemical Sciences 7:431–433
    [Google Scholar]
  10. Matsumoto H., Hosogaya S., Suzuki K., Tazaki T. 1975; Arginine gene cluster of Serratia marcescens. Japanese Journal of Microbiology 19:35–44
    [Google Scholar]
  11. Measures J. C. 1975; Role of amino acids in osmoregulation of non-halophilic bacteria. Nature, London 257:398–400
    [Google Scholar]
  12. Roller S. O., Anagnostopoulos G. O. 1982; Accumulation of carbohydrate by Escherichia coli B/r/1 during growth at low water activity. Journal of Applied Bacterioloty 52:425–434
    [Google Scholar]
  13. Rosenfeld S. A., Brenchley J. E. 1983; Regulation of glutamate and glutamine biosynthesis. In Amino Acids: Biosynthesis and Genetic Regulation1–17 Edited by Herrmann K., Somerville R. L. Reading, Massachusetts: Addison-Wesley;
    [Google Scholar]
  14. Steele B. F., Sauberlich H. E., Reynolds M. S., Baumann C. A. 1949; Media for Leuconostoc mesenteroides P-60 and Leuconostoc citrovorum 8081. Journal of Biological Chemistry 177:533–544
    [Google Scholar]
  15. Sugiura M., Kisumi M. 1984; Stabilization of a histidine-producing strain of Serratia marcescens. Applied and Environmental Microbiology 48:43–47
    [Google Scholar]
  16. Sugiura M., Klsumi M. 1985; Proline-hyperproducing strains of Serratia marcescens: enhancement of proline analog-mediated growth inhibition by increasing osmotic stress. Applied and Environmental Microbiology 49:782–786
    [Google Scholar]
  17. Sugiura M., Kisumi M., Chibata I. 1981; Biosynthetic pathway of β-methylnorleucine, an antimetabolite produced by Serratia marcescens. Journal of Antibiotics 34:1283–1289
    [Google Scholar]
  18. Sugiura M., Takagi T., Kisumi M. 1985; Proline production by regulatory mutants of Serratia marcescens. Applied Microbiology and Biotechnology 21:213–219
    [Google Scholar]
  19. Tempest O. W., Meers J. L., Brown C. M. 1970; Influence of environment on the content and composition of microbial free amino acid pools. Journal of General Microbiology 64:171–185
    [Google Scholar]
  20. Thompson J., Macleod R. A. 1974; Potassium transport and the relationship between intracellular potassium concentration and amino acid uptake by cells of a marine pseudomonad. Journal of Bacteriology 120:598–603
    [Google Scholar]
  21. Umbarger H. E. 1978; Amino acid biosynthesis and its regulation. Annual Review of Biochemistry 49:1005–1061
    [Google Scholar]
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