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Volume 131, Issue 10

Environmental Regulation of Carbohydrate Metabolism by NCTC 7865 Grown in a Chemostat Free

Abstract

Summary: Carbohydrate metabolism by the oral bacterium NCTC 7865 was studied using cells grown in a chemostat at pH 7.0 under glucose or amino acid limitation (glucose excess) over a range of growth rates ( = 0.05 h−0.4 h). A mixed pattern of fermentation products was always produced although higher concentrations of lactate were formed under amino acid limitation. Analysis of culture filtrates showed that arginine was depleted from the medium under all conditions of growth; a further supplement of 10 m-arginine was also consumed but did not affect cell yields, suggesting that it was not limiting growth. Except at the slowest growth rate ( = 0.05 h) under glucose limitation, the activity of the glucose phosphotransferase (PTS) system was insufficient to account for the glucose consumed during growth, emphasizing the importance of an alternative method of hexose transport in the metabolism of oral streptococci. The PTS for a number of sugars was constitutive in NCTC 7865 and, even though the cells were grown in the presence of glucose, the activity of the sucrose-PTS was highest. The glycolytic activity of cells harvested from the chemostat was affected by the substrate, the pH of the environment, and their original conditions of growth. Glucose-limited cells produced more acid than those grown under conditions of glucose excess; at slow growth rates, in particular, greater activities were obtained with sucrose compared with glucose or fructose. Maximum rates of glycolytic activity were obtained at pH 8.0 (except for cells grown at = 0.4 h where values were highest at pH 7.0), while slow-growing, amino acid-limited cells could not metabolize at pH 5.0. These results are discussed in terms of their possible significance in the ecology of dental plaque and the possible involvement of these bacteria in the initiation but not the clinical progression of a carious lesion.

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© Society for General Microbiology 1985
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1985-10-01
2024-04-27
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References

  1. Carlsson J. 1970; Nutritional requirements of Streptococcus mutans. Caries Research 4:305–320
     [Google Scholar]
  2. Dawes E. A. 1976; Endogenous me.tabolism and the survival of starved prokaryotes. Symposia of the Society for General Microbiology 26:19–53
     [Google Scholar]
  3. Drucker D. B., Green R. M. 1981; The relative cariogenicity of different streptococci in the gnotohiotic WAG RIJ rat. Archires of Oral Biology 26:599–606
     [Google Scholar]
  4. Ellwood D. C., Hamilton I. R. 1982; Properties of Streptococcus mutans Ingbritt growing on limiting sucrose in a chemostat: repression of the phosphoenolpyruvate phosphotransferase system. Infection and Immunity 36:576–581
     [Google Scholar]
  5. Ellwood D. C., Hunter J. R., Longyear V. M. C. 1974; Growth of Strep1ococcus mutans in a chemostat. Archives of Oral Biology 19:659–665
     [Google Scholar]
  6. Ellwood D. C., Phipps P. J., Hamilton I. R. 1979; Effect of growth rate and glucose concentration on the activity of the phosphoenolpyruvate phosphotransferase system in Streptococcus mutans lngbritt grown in continuous culture. Infection and Immunity 23:224–231
     [Google Scholar]
  7. Featherstone J. D. B., Rodgers B. E. 1981; Effect of acetic. lactic and other organic acids on the formation of artificial carious lesions. Caries Research 15:377–385
     [Google Scholar]
  8. Geddes D. A. M., Weetman D. A., Featherstone J. D. B. 1984; Preferential loss of acetic acid from plaque fermentation in the presence of enamel. Caries Research 18:430–433
     [Google Scholar]
  9. Hamilton I. R., Ellwood D. C. 1978; Effects of fluoride on carbohydrate metabolism by washed cells of Streptoeoccus mutans grown at various pH values in a chemostat. Infection and Immunity 19:434–442
     [Google Scholar]
  10. Hamilton I. R., Ellwood D. C. 1983; Carbohydrate metabolism by Actinomyces riscosus growing in continuous culture. Injection and Immunity 42:19–26
     [Google Scholar]
  11. Hamilton I. R. Sr., Martin E. J. 1982; Evidence for the involbement of proton motive force in the transport of glucose by a mutant of Streptococcus mutans strain DR 0001 defective in glucose-phosphoenolpqruvate phosphotransferase activity. Infection and Immunity 36:567–575
     [Google Scholar]
  12. Hardie J. M., Thomson P. T., South R. J., Marsh P. D., Bowden G. H., Mckee A. S., Fillery E. D., Slack G. L. 1977; A longitudinal epidemiological study of dental plaque and the development of dental caries – interim results after two years. Journal of Dental Research 56:90–98
     [Google Scholar]
  13. Harper D. S., Loesche W. J. 1984; Growth and acid tolerance of human dental plaque bacteria. Archives of Oral Biology 29:843–848
     [Google Scholar]
  14. Herbert D., Kornberg H. L. 1976; Glucose transport as a rate-limiting step in the growth of Escherichia coli on glucose. Biochemical Journal 156:477–480
     [Google Scholar]
  15. Huis In T’Veld J., Van Palenstein-Helderman W. H., Backer Dirks O. 1979; Streptococcus mutans and dental decay in humans-a bacteriological and immunological study. Antonie zan Leeuwenhoek 45:25–33
     [Google Scholar]
  16. Keevil C. W., West A. A., Marsh P. D., Ellwood D. C. 1983; Batch versus continuous culture studies of glucosyltransferase synthesis in oral streptococci. In Glucosyltransferase, Glucans, Sucrose and Demal Caries pp 189–200 Edited by Doyle R. J., Ciardi J. E. Washington, DC: Information Retrieval:
     [Google Scholar]
  17. Keevil C. W., Marsh P. D., Ellwood D. C. 1984a; Regulation of glucose metabolism in oral streptococci through independent pathways of glucose 6-phosphate and glucose I-phosphate formation. Journal of Bacteriology 157:560–567
     [Google Scholar]
  18. Keevil C. W., West A. A., Bourne N., Marsh P. D. 1984b; Inhibition of the synthesis and secretion of extracellular glucosyl-and fructosyxstransferase in Streptococcus sanguis by sodium ions. Journal of General Microbiology 130:77–82
     [Google Scholar]
  19. Keevil C. W., Williamson M. I., Marsh P. D., Ellwood D. C. 1984c; Evidence that glucose and sucrose uptake in oral streptococcal bacteria involves independent phosphotransferase and proton motive force-mediated mechanisms. Archives of Oral Biology 29:871–878
     [Google Scholar]
  20. Kemp C. W., Robrish S. A., Curtis M. A., Sharer S. A., Bowen W. H. 1983; Application of a competition model to the growth of Streptococcus mutans and Streptococcus sanguis in binary continuous culture. Applied and Environmental Microbiology 45:1277–1282
     [Google Scholar]
  21. Loesche W. J., Straffon L. H. 1979; Longitudinal investigations of the role of Streptococcus mutans in human fissure decay. lnJection and Immunity 26:498–507
     [Google Scholar]
  22. Mckee A. S., Mcdermid A. s., Marsh P. D., Ellwood D. C. 1984; The application ofcontinuous culture techniques to the study of oral anaerobes. In Models of Anaerobic Infection pp 165–175 Edited by Hill M., Borriello S. P., Hardie J. M., Hudson M. J., Lysons R. L., Tabaqchali S. Dordrecht: Martinus Nijhoff;
     [Google Scholar]
  23. Marsh P. D., Martin M. 1984 Oral Microbiology, 2nd. edn Wokingham: van Nostrand Reinhold;
     [Google Scholar]
  24. Marsh P. D., Williamson M. I., Keevil C. W., Mcdermid A. S., Ellwood D. C. 1982; The influence of sodium and potassium ions on acid production by washed cells of Streptococcus mutans Ingbritt and Streptococcus sanguis NCTC 7865 grown in a chemostat. Infection and Immunity 36:476–483
     [Google Scholar]
  25. Marsh P. D., Hunter J. R., Bowden G. H., Hamilton I. R., Mckee A. S., Hardie J. M., Ellwood D. C. 1983; The influence of growth rate and nutrient limitation on the microbial composition and biochemical properties of a mixed culture of oral bacteria grown in a chemostat. Journal of General Microbiology 129:755–770
     [Google Scholar]
  26. Marsh P. D., Keevil C. W., Ellwood D. C. 1984; Relationship of bioenergetic processes to the pathogenic properties of oral bacteria. Journal of Dental Research 63:401–406
     [Google Scholar]
  27. Stmartin E. J., Wittenberger C. L. 1979; Regulation and function of sucrose 6-phosphate hydrolase in Streptococcus mutans. Infection and Immunity 26:487–491
     [Google Scholar]
  28. Slee A. M., Tanzer J. M. 1980; Effect of growth conditions on sucrose phosphotransferase activity of Streptococcus mutans. Infection and immunity 27:922–927
     [Google Scholar]
  29. Tanzer J. M., Freedman M. L., Woodiel F. N., Eifert R. L., Rinehimer L. A. 1976; Association of Streptococcus mutans virulence with synthesis of intracellular polysaccharide. In Microbial Aspects of Dental Caries 3 pp 597–616 Edited by Stiles H. M., Loesche W. J., O’Brien T. C. Washington DC: Information Retrieval;
     [Google Scholar]
  30. Van Houte J. 1980; Bacterial specificity in the etiology of dental caries. International Dental Journal 30:305–326
     [Google Scholar]
  31. West A. A., Keevil C. W., Marsh P. D., Ellwood D. C. 1984; The effect of ionophores on growth and glycosyltransferase production of Streptococcus sanguis. FEMS Microbiologr Letters 25:133–137
     [Google Scholar]
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Environmental Regulation of Carbohydrate Metabolism by Streptococcus sanguis NCTC 7865 Grown in a Chemostat
Microbiology 131, 2505 (1985); https://doi.org/10.1099/00221287-131-10-2505
/content/journal/micro/10.1099/00221287-131-10-2505
/content/journal/micro/10.1099/00221287-131-10-2505
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