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SUMMARY: Fatty acids of 16 strains of Selenomonas isolated from the human oral cavity were examined by gas-liquid chromatography. The strains showed similar patterns, characterized by the presence of straight-chain fatty acids in the range C11 to C18. Fatty acids of odd-numbered carbon atoms dominated and the major acids were n-pentadecanoate and 3-hydroxytridecanoate. The general fatty acid pattern of Selenomonas differed distinctly from those of other previously analysed anaerobic or microaerophilic Gram-negative bacilli.