1887

Abstract

Treatment of with bleomycin induced a dramatic increase in ATP concentration in the first 30 min. Afterwards, in RecA strains, ATP dropped quickly to values similar to those of untreated cells. Mutants of defective in either RecA protein or RecA protease activity did not show this decrease, indicating that it was due to the action of RecA protease. The increase in ATP in the first 30 min was dependent on RecBC exonuclease activity and must have been due to substrate level phosphorylation, since an uncoupler such as dinitrophenol did not affect it. Nevertheless, mitomycin C did not induce any change in ATP pools of RecA strains, at least during 120 min following treatment. The implications of these findings are discussed in relation to the possible pathways of activation of RecA protease.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-9-2247
1984-09-01
2021-07-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/9/mic-130-9-2247.html?itemId=/content/journal/micro/10.1099/00221287-130-9-2247&mimeType=html&fmt=ahah

References

  1. Barbé J., Vericat J. A., Guerrero R. 1983a; Discriminated induction of SOS functions in Escherichia coti by alkylating agents. Journal of General Microbiology 129:2079–2089
    [Google Scholar]
  2. Barbé J., Villaverde A., Guerrero R. 1983b; Evolution of cellular ATP concentration after UV- mediated induction of SOS system in Escherichia coli. Biochemical and Biophysical Research Communications 117:556–561
    [Google Scholar]
  3. Barbé J., Vericat J. A., Guerrero R. 1983c; recA-Dependent inhibition of cell respiration is not induced by mitomycin C in Escherichia coli. Mutation Research 120:1–5
    [Google Scholar]
  4. Brent R., Ptashne M. 1981; Mechanism of action of the lex A gene product. Proceedings of the National Academy of Sciences of the United States of America 78:3700–3704
    [Google Scholar]
  5. Casaregola S., D’ARI R., Huisman O. 1982; Role of DNA replication in the induction and turnoff of the SOS response in Escherichia coli. Molecular and General Genetics 185:440–444
    [Google Scholar]
  6. Chapman A. G., Fall L., Atkinson D. E. 1971; Adenylate charge in Escherichia coli during growth and starvation. Journal of Bacteriology 108:1072–1086
    [Google Scholar]
  7. Craig N. L., Roberts J. W. 1981; Function of nucleoside triphosphate and polynucleotide in Escherichia coli recA protein-directed cleavage of phage λ repressor. Journal of Biological Chemistry 256:8039–8044
    [Google Scholar]
  8. Guerrero R., Barbé J. 1982; Expression of recA-gene dependent SOS functions in Salmonella typhi- murium. Antonie van Leeuwenhoek 48:159–167
    [Google Scholar]
  9. Howard-FLANDERS P., Theriot L. 1962; A method for selecting radiation sensitive mutants of Escherichia coli. Genetics 47:1219–1224
    [Google Scholar]
  10. Howard-FLANDERS P., Theriot L. 1966; Mutants of Escherichia coli K.12 defective in DNA repair and in genetic recombination. Genetics 53:1137–1150
    [Google Scholar]
  11. Little J. W., Mount D. W. 1982; The SOS regulatory system of Escherichia coli. Cell 29:11–22
    [Google Scholar]
  12. Little J. W., Mount D. W., Yanisch-PERRON C. R. 1981; Purified lex A protein is a repressor of the recA and lexA genes. Proceedings of the National Academy of Sciences of the United States of America 78:4199–4203
    [Google Scholar]
  13. Miller J. H. 1972 Experiments in Molecular Genetics. New York; Cold Spring Harbor Laboratory:
    [Google Scholar]
  14. Witkin E. M. 1976; Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriological Reviews 40:869–907
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-9-2247
Loading
/content/journal/micro/10.1099/00221287-130-9-2247
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error