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A 6·95 kb HindIII-generated DNA fragment from Bacillus subtilis 168 was inserted into the DNA of phage ϕ105DI:lt. The recombinant phage (ϕ105DS1) contained DNA of 33·8 kb as compared with 35·2 kb for ϕ105DI:lt and 39·2 kb for the wild-type phage. In the presence of helper phage, ϕ105DS1 complemented both spoIIA and spoVA mutations in B. subtilis.
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