1887

Abstract

Cells of underwent rapid autolysis when suspended in media of low osmolarity under non-growing conditions. Chaotropes like urea and guanidine. HCl which are potent protein denaturants caused complete and immediate lysis of whole cells. This unique sensitivity of to protein denaturants led to the development of a rapid method for the selective isolation of the outer membrane upon treatment of whole cells with urea. The composition of the outer membrane isolated from both whole cells and crude envelopes by treatment with urea was comparable with that of the outer membrane isolated by other conventional methods.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-8-2027
1984-08-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/8/mic-130-8-2027.html?itemId=/content/journal/micro/10.1099/00221287-130-8-2027&mimeType=html&fmt=ahah

References

  1. Adhikari P. C., Raychaudhuri C., Chatterjee S. N. 1969; The lysis of cholera and El Tor vibrios. Journal of General Microbiology 59:91–95
    [Google Scholar]
  2. Ames B. N., Dubin T. 1960; The role of polyamines in the neutralisation of bacteriophage deoxyribonucleic acid. Journal of Biological Chemistry 235:769–775
    [Google Scholar]
  3. Bligh G. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  4. Dirienzo J. M., Nakamura K., Inouye M. 1978; The outer membrane proteins of Gram-negative bacteria.Biosynthesis, assembly and functions. Annual Review of Biochemistry 47:481–532
    [Google Scholar]
  5. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  6. Fernandes P. B., Bayer M. E. 1977; Membrane-bound enterotoxin of Vibrio cholerae. Journal of General Microbiology 103:381–387
    [Google Scholar]
  7. Filip C., Fletcher G., Wulf J. L., Earhart C. F. 1973; Solubilisation of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium lauryl sarcosinate. Journal of Bacteriology 115:717–722
    [Google Scholar]
  8. Ghuysen J. M. 1968; Use of bacteriolytic enzymes in determination of wall structure and their role in cell metabolism. Bacteriological Reviews 32:425–464
    [Google Scholar]
  9. Griffith O. M. 1978; Rapid density gradient centrifugation using short column techniques. Analytical Biochemistry 90:435–443
    [Google Scholar]
  10. Hager L. P., Deeb S. S. 1967; The preparation of bacterial cytochrome b1 . Methods in Enzymology 10:367–372
    [Google Scholar]
  11. Hartmann R., Bock-Hennig S. B., Schwarz U. 1974; Murein hydrolases in the envelope of Escherichia coli. Properties in situ and solubilisation from the envelope. European Journal of Biochemistry 41:203–208
    [Google Scholar]
  12. Horio T., Higashi T., Sasagarwa M., Kusai K., Nakai M., Okanuki K. 1960; Preparation of crystalline Pseudomonas cytochrome C-551 and its general properties. Biochemical Journal 77:194–201
    [Google Scholar]
  13. Ingram L. O. 1981; Mechanism of lysis of Escherichia coli by ethanol and other chaotropic agents. Journal of Bacteriology 146:331–336
    [Google Scholar]
  14. Jann B., Reske K., Jann K. 1975; Heterogeneity of lipopolysaccharides.Analysis of polysaccharide chain lengths by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. European Journal of Biochemistry 60:239–246
    [Google Scholar]
  15. Kabir S. 1980; Composition and immunochemical properties of outer membrane proteins of Vibrio cholerae. Journal of Bacteriology 144:382–389
    [Google Scholar]
  16. Kelley J. T., Parker C. D. 1981; Identification and preliminary characterisation of Vibrio cholerae outer membrane proteins. Journal of Bacteriology 145:1018–1024
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  18. Larsen H. 1967; Biochemical aspects of extreme halophilism. Advances in Microbial Physiology 1:97–132
    [Google Scholar]
  19. Lugtenberg B., Van Alphen L. 1983; Molecular architecture and functioning of the outer membrane of Escherichia coli and other Gram-negative bacteria. Biochimica et biophysica acta 737:51–115
    [Google Scholar]
  20. Markwell M. A. K, Haas S. M., Bieber L. L., Tolbert N. E. 1978; A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Analytical Biochemistry 87:206–210
    [Google Scholar]
  21. Nikaido H. 1979; Non specific transport through the outer membrane. In Bacterial Outer Membranes pp. 361–407 Edited by Inouye M. New York: John Wiley & Sons;
    [Google Scholar]
  22. Pearson G. D. N, Mekalanos J. J. 1982; Molecular cloning of Vibrio cholerae enterotoxin genes in Escherichia coli K-12. Proceedings of the National Academy of Sciences of the United States of America 79:2976–2980
    [Google Scholar]
  23. Raziuddin S., Kawasaki T. 1976; Biochemical studies on the cell wall lipopolysaccharides (O-Anti-gens) of Vibrio cholerae 569B (Inaba) and El Tor (Inaba). Biochimica et biophysicaacta 431:116–126
    [Google Scholar]
  24. Repaske R. 1956; Lysis of Gram-negative bacteria by lysozyme. Biochimica et biophysicaacta 22:189–191
    [Google Scholar]
  25. Schnaitman C. A. 1970; Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli. Journal of Bacteriology 104:890–901
    [Google Scholar]
  26. Stewart-TULL D. E. S. 1965; A modified PAS staining technique for polysaccharides in gels. Immunology 8:221–222
    [Google Scholar]
  27. Sur P., Chatterjee S. N. 1979; Molecular weight and analysis of protein units in Vibrio cholerae cell envelope. Journal of General and Applied Microbiology 21:61–63
    [Google Scholar]
  28. Suzuki H., Nishimura Y., Yasuda S., Nishimura A., Yamada M., Hirota Y. 1978; Murein lipoprotein of Escherichia coli: a protein involved in the stabilisation of bacterial cell envelope. Molecular and General Genetics 167:1–9
    [Google Scholar]
  29. Weibull C. 1953; The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. Journal of Bacteriology 66:688–695
    [Google Scholar]
  30. Weidel W., Frank H., Martin H. H. 1960; The rigid layer of the cell wall of Escherichia coli strain B. Journal of General Microbiology 22:158–166
    [Google Scholar]
  31. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides.Extraction with phenol-water and further applications of the procedure. Methods in Carbohydrate Chemistry 5:83–91
    [Google Scholar]
  32. Winter A. J., Katz W., Martin H. H. 1971; Murein (peptidoglycan) structure of Vibrio fetus comparison of a veneral and an intestinal strain. Biochimica et biophysica acta 244:58–64
    [Google Scholar]
  33. Wright B. E., Rebers P. A. 1972; Procedure for determining heptose and hexose in lipopolysaccharides.Modification of the cysteine-sulphuric acid method. Analytical Biochemistry 49:307–319
    [Google Scholar]
  34. Yem D. W., Wu H. C. 1978; Physiological characterization of an Escherichia coli mutant altered in the structure of murein lipoprotein. Journal of Bacteriology 133:1419–1426
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-8-2027
Loading
/content/journal/micro/10.1099/00221287-130-8-2027
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error