Protoplasts from hyphae of . collected from lytic medium after different times of incubation, represented a sequential fractionation of mycelium from apical to distal zones. This method gives the opportunity to study the intrahyphal localization of cell wall glucan synthases in relation to apical growth. (l→4)-β-Glucan synthases were mainly found in early protoplasts (i.e. from apical zones) while the highest (l→3)-β-glucan synthase activities were recovered in late protoplasts (i.e. from the subapical zones). Early protoplasts also exhibited the highest rate of cell wall synthesis during regeneration. The distribution of the enzymes along the fungal hyphae might provide a clue to the mechanisms of cell wall polysaccharide deposition during apical growth.


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