1887

Abstract

Pyruvate and ethanol were both effective electron donors for nitrite reduction by K12. The pyruvate-dependent rate decreased by approximately 50% when either a mutation, which results in loss of NADH-dependent nitrite reductase activity (EC 1.6.6.4), or a mutation, which results in loss of the formate-nitrite oxidoreductase activity, was introduced into the prototrophic parental strain CGSC4315. A double mutant deficient in both of these previously described activities retained only 2% of the rate of nitrite reduction of the parental strain after growth on glucose or 5% after growth on pyruvate. We conclude that any third pathway for nitrite reduction contributes little to the rate of nitrite reduction by wild-type strains.

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1984-05-01
2021-10-25
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References

  1. Abou-Jaoudé A., Chippaux M., Pascal M. C., Casse F. 1977; Formate: a new electron donor for nitrite reduction in Escherichia coli K12. Biochemical and Biophysical Research Communications 78:579–583
    [Google Scholar]
  2. Abou-Jaoudé A., Chippaux M., Pascal M. C. 1979; Formate-nitrite reduction in Escherichia coli K12. Physiological study of the system. European Journal of Biochemistry 95:309–314
    [Google Scholar]
  3. Cole J. A., Wimpenny J. W. T. 1968; Metabolic pathways for nitrate reduction in Escherichia coli. Biochimica et biophysica acta 162:39–48
    [Google Scholar]
  4. Cole J. A., Coleman K. J., Compton B. E., Kavanagh B. M., Keevil C. W. 1974; Nitrite and ammonia assimilation by anaerobic continuous cultures of Escherichia coli. Journal of General Microbiology 85:11–22
    [Google Scholar]
  5. Fujita T. 1966; Studies on soluble cytochromes in the Enterobacteriaceae. I. Detection, purification and properties of cytochrome c552 in anaerobically grown cells. Journal of Biochemistry 60:204–215
    [Google Scholar]
  6. Jackson R. H., Cornish-Bowden A., Cole J. A. 1981; Prosthetic groups of the NADH-dependent nitrite reductase from Escherichia coli K12. Biochemical Journal 193:861–867
    [Google Scholar]
  7. Kaspar H. F., Tiedje J. M. 1981; Dissimilatory reduction of nitrate and nitrite in the bovine rumen: nitrous oxide production and effect of acetylene. Applied and Environmental Microbiology 41:705–709
    [Google Scholar]
  8. Kemp J. D., Atkinson D. E. 1966; Nitrite reductase of Escherichia coli specific for reduced nicotinamide adenine dinucleotide. Journal of Bacteriology 92:628–634
    [Google Scholar]
  9. Kersters K., De Ley J. 1966; Soluble NAD-linked primary and secondary alcohol dehydrogenases. Methods in Enzymology 9:347–350
    [Google Scholar]
  10. Knappe J., Blaschkowski H. P., Grobner P., Schmitt T. 1974; Pyruvate-formate lyase of Escherichia coli: the acetyl enzyme intermediate. European Journal of Biochemistry 50:253–263
    [Google Scholar]
  11. Kornberg H. L. 1967; Genetic control of the uptake of pyruvate by Escherichia coli. Biochimica et biophysica acta 148:591–592
    [Google Scholar]
  12. Newman B. M., Cole J. A. 1978; The chromosomal location and pleiotropic effects of mutations of the tiirA+ gene of Escherichia coli K12: the essential role of nirA + in nitrite reduction and in other anaerobic redox reactions. Journal of General Microbiology 106:1–12
    [Google Scholar]
  13. Pascal M. C., Chippaux M., Abou-Jaoudé A., Blaschkowski H. P., Knappe J. 1981; Mutants of Escherichia coli K12 with defects in anaerobic pyruvate metabolism. Journal of General Microbiology 124:35–42
    [Google Scholar]
  14. Pope N. R., Cole J. A. 1982; Generation of a membrane potential by one of two independent pathways for nitrite reduction by Escherichia coli. Journal of General Microbiology 128:219–222
    [Google Scholar]
  15. Satoh T., Hom S. S. M, Shanmugam K. T. 1983; Production of nitrous oxide from nitrite in Klebsiella pneumoniae: mutants altered in nitrogen metabolism. Journal of Bacteriology 155:454–458
    [Google Scholar]
  16. Smith M. S. 1983; Nitrous oxide production of Escherichia coli is correlated with nitrate reductase activity. Applied and Environmental Microbiology 45:1545–1547
    [Google Scholar]
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