1887

Abstract

The outer membrane proteins of five clinical isolates of were identified by I-surface labelling and SDS-PAGE of outer membrane preparations. All isolates expressed a major outer membrane protein of variable molecular weight (43000–46000: 43K–46K). Several constant surface proteins were also identified including a 27K protein which was surface-exposed and acid-extractable but was not present in the outer membrane preparations. Isolated flagella comprised a major 62K protein and a minor 87K protein. Both proteins were absent in an aflagellate variant. The 62K protein was immunoblotted and immunoprecipitated by rabbit anti-flagella antisera.

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1984-05-01
2022-01-19
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References

  1. Arrigoni O., Singer T. P. 1962; Limitations of the phenazine methosulphate assay for succinic and related dehydrogenases. Nature, London 193:1256–1258
    [Google Scholar]
  2. Belland R. J., Trust T. J. 1982; Deoxyribonucleic acid sequence relatedness between thermophilic members of the genus Campylobacter. Journal of General Microbiology 128:2515–2522
    [Google Scholar]
  3. Butzler J. P., Skirrow M. B. 1979; Campylobacter enteritis. Clinics in Gastroenterology 8:737–765
    [Google Scholar]
  4. Churchward G. G., Holland I. B. 1976; Envelope synthesis during the cell cycle in Escherichia coli B/r. Journal of Molecular Biology 105:245–261
    [Google Scholar]
  5. Davidson J. A., Solomon J. B. 1980; Onset of resistance to pathogenic strains of Campylobacter fetus subspecies jejuni in the chick embryo. In Aspects of Developmental and Comparative Immunology - I pp. 289–294 Edited by Solomon J. B. Oxford: Pergamon Press;
    [Google Scholar]
  6. Der-Balian G. P. 1980; Imidoester iodination of immunoglobulins to high specific activity. Analytical Biochemistry 106:411–416
    [Google Scholar]
  7. Filip C., Fletcher G., Wulff J. F., Earhart C. F. 1973; Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium lauryl sarcosinate. Journal of Bacteriology 115:717–722
    [Google Scholar]
  8. Giannella R. A. 1981; Pathogenesis of acute bacterial diarrhoeal disorders. Annual Review of Medicine 32:341–357
    [Google Scholar]
  9. Guerrant R. L., Lahita R. G., Winn W. C., Roberts R. B. 1978; Campylobacteriosis in man: pathogenic mechanisms and review of 91 bloodstream infections. American Journal of Medicine 65:584–592
    [Google Scholar]
  10. Kondah H., Hotani H. 1974; Flagellin from Escherichia coli K/2. Polymerization and molecular weight in comparison with Salmonella flagellins. Biochimica et biophysica acta 336:117–139
    [Google Scholar]
  11. Lambden P. R., Heckels J. E., James L. T., Watts P. J. 1979; Variations in surface protein composition associated with virulence properties in opacity types of Neisseria gonorrhoeae. Journal of General Microbiology 114:305–312
    [Google Scholar]
  12. Lawn A. M. 1977; Comparison of the flagellins from different flagellar morphotypes of Escherichia coli. Journal of General Microbiology 101:112–130
    [Google Scholar]
  13. Logan S. M., Trust T. J. 1982; Outer membrane characteristics of Campylobacter jejuni. Infection and Immunity 38:898–906
    [Google Scholar]
  14. Manninen K. I., Prescott J. F., Dohoo I. R. 1982; Pathogenicity of Campylobacter jejuni isolates from animals and humans. Infection and Immunity 38:46–52
    [Google Scholar]
  15. Marchalonis J. J., Cone R. E., Santer V. 1971; A probe for accessible surface proteins of normal and neoplastic lymphocytes. Biochemical Journal 124:921–927
    [Google Scholar]
  16. Mccoy E. C., Doyle D., Burda K., Corbeil L. B., Winter A. J. 1975; Superficial antigens of Campylobacter (Vibrio) fetus: characterisation of an antiphagocytic component. Infection and Immunity 11:517–525
    [Google Scholar]
  17. Mccoy E. C., Wiltberger H. A., Winter A. J. 1976; Major outer membrane protein of Campylobacter fetus:physical and immunological characterisation. Infection and Immunity 13:1258–1265
    [Google Scholar]
  18. Newell D. G. 1983; The surface protein antigens, including flagella of Campylobacter, jejuni. In Campylobacter II pp. 66 Edited by Pearson A. D., Skirrow M. B., Rowe B., Davis J. R., Jones D. M. London: P.H.L.S.;
    [Google Scholar]
  19. Owen R. J., Leaper S. 1981; Base composition, size and nucleotide sequence similarities of genome deoxyribonucleic acids from species of the genus Campylobacter. FEMS Microbiology Letters 12:395–400
    [Google Scholar]
  20. Palmer S. R., Gully P. R., White J. M., Pearson A. D., Suckling W. G., Jones D. M., Rawes J. C. L, Penner J. L. 1983; Water-borne outbreak of Campylobacter gastroenteritis. Lancet i:287–290
    [Google Scholar]
  21. Penner J. L., Hennessy J. N. 1980; Passive haemagglutination technique for serotyping Campylobacter fetus subspecies jejuni on the basis of soluble heat-stable antigens. Journal of Clinical Microbiology 12:732–737
    [Google Scholar]
  22. Rautelin H., Kosunen T. U. 1983; An acid extract as a common antigen in Campylobacter coli and Campylobacter jejuni strains. Journal of Clinical Microbiology, 17:700–701
    [Google Scholar]
  23. Skirrow M. B. 1977; Campylobacter enteritis: a ‘new’ disease. British Medical Journal 2:9–11
    [Google Scholar]
  24. Skirrow M. B. 1982; Campylobacter enteritis - the first five years. Journal of Hygiene 89:175–184
    [Google Scholar]
  25. Skirrow M. B., Benjamin J. 1980; Differentiation of enteropathogenic Campylobacter. Journal of Clinical Pathology 33:1122
    [Google Scholar]
  26. Skirrow M. B., Benjamin J. 1982; The classification of ‘thermophilic’ Campylobacters and their distribution in man and domestic animals. In Campylobacter: Epidemiology, Pathogenesis and Biochemistry pp. 40–44 Edited by Newell D. G. Lancaster: MTP Press;
    [Google Scholar]
  27. Swanson J. 1981; Surface exposed protein antigens of the gonococcal outer membrane. Infection and Immunity 34:804–816
    [Google Scholar]
  28. Walder M., Forsgren A. 1982; Enzyme-linked immunosorbent assay (ELISA) for antibodies against Campylobacter jejuni and its clinical application. Acta pathologica, microbiologica et immunologica scandinavica 90:423–433
    [Google Scholar]
  29. Waravdekar V. S., Saslaw L. D. 1959; A sensitive colorimetric method for the estimation of 2- deoxy sugars with the use of the malonaldehyde thiobarbituric acid reaction. Journal of Biological Chemistry 234:1945–1950
    [Google Scholar]
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