Summary: EDTA extracts were prepared from whole cells of 16 strains of (types A-E), 6 strains of (types A-D) and 3 strains of . They were reacted in an enzyme-linked immunosorbent assay (ELISA) with antisera raised against whole, UV-killed cells of and type A. Results showed significant cross-reactions between antiserum and the type A (three out of four strains), proteolytic type B (all strains) and one type E strain, and between type A antiserum and types C and D. All the and strains reacted with their homologous antiserum; these two species showed no cross-reactions. All the reactions were investigated further by running the EDTA extracts on SDS-polyacrylamide gels. The separated molecules were electro-phoretically transferred to nitrocellulose membranes, reacted with antiserum and complexes visualized with horseradish peroxidase conjugate reagents. Only those extracts that reacted significantly in the ELISA gave a pattern of cross-reactive antigen bands and the number of bands and intensity of stain closely paralleled the strength of the ELISA reaction.


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