1887

Abstract

EDTA extracts were prepared from whole cells of 16 strains of (types A-E), 6 strains of (types A-D) and 3 strains of . They were reacted in an enzyme-linked immunosorbent assay (ELISA) with antisera raised against whole, UV-killed cells of and type A. Results showed significant cross-reactions between antiserum and the type A (three out of four strains), proteolytic type B (all strains) and one type E strain, and between type A antiserum and types C and D. All the and strains reacted with their homologous antiserum; these two species showed no cross-reactions. All the reactions were investigated further by running the EDTA extracts on SDS-polyacrylamide gels. The separated molecules were electro-phoretically transferred to nitrocellulose membranes, reacted with antiserum and complexes visualized with horseradish peroxidase conjugate reagents. Only those extracts that reacted significantly in the ELISA gave a pattern of cross-reactive antigen bands and the number of bands and intensity of stain closely paralleled the strength of the ELISA reaction.

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/content/journal/micro/10.1099/00221287-130-4-975
1984-04-01
2022-01-19
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References

  1. Eklund M. W., Poysky F. T. 1981; Relationships of bacteriophages to the toxigenicity of Clostridium botulinum and closely related organisms. In Biomedical Aspects of Botulism pp. 93–109 Lewis G. E. Edited by New York: Academic Press;
    [Google Scholar]
  2. Hatheway C. L., Mccroskey L. M. 1981; Laboratory investigation of human and animal botulism. In Biomedical Aspects of Botulism pp. 165–180 Lewis G. E. Edited by New York: Academic Press;
    [Google Scholar]
  3. Holbrook W. P., Duerden B. I., Deacon A. G. 1977; The classification of Bacteroides melaninogenicus and related species. Journal of Applied Bacteriology 42:259–273
    [Google Scholar]
  4. Holdeman L. V., Cato E. P., Moore W. E. C. editors 1977 Anaerobe Laboratory Manual, 4th edn.. Blacksburg, Va., USA: Anaerobe Laboratory, Virginia Polytechnic Institute and State University;
    [Google Scholar]
  5. Iida H., Oguma K., Inoue K. 1981; Toxin production and phage in Clostridium botulinum types C and D. In Biomedical Aspects of Botulism p. 119 Lewis G. E. Edited by New York: Academic Press;
    [Google Scholar]
  6. Kiritani K., Mitsui S., Nakamura S., Nishida S. 1973; Numerical taxonomy of Clostridium botulinum and Clostridium sporogenes strains, and their susceptibilities to induced lysins and to mitomycinC. Japanese Journal of Microbiology 17:361–372
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 277680–685
    [Google Scholar]
  8. Poxton I. R. 1979; Serological identification of Bacteroidesspecies by an enzyme-linked immunosorbent assay. Journal of Clinical Pathology 32:294–298
    [Google Scholar]
  9. Poxton I. R., Brown R. 1979; Sodium dodecyl sulphate-polyacrylamide gel-electrophoresis of cell-surface proteins as an aid to the identification of the Bacteroides fragilis group. Journal of General Microbiology 112:211–217
    [Google Scholar]
  10. Poxton I. R., Byrne M. D. 1981; Immunological analysis of the EDTA-soluble antigens of Clostridium difficile and related species. Journal of General Microbiology 122:41–46
    [Google Scholar]
  11. Poxton I. R., Byrne M. D. 1984; The demonstration of shared antigens in the genus Clostridium by an enzyme-linked immunosorbent assay. Journal of Medical Microbiology in the Press
    [Google Scholar]
  12. Poxton I. R., Cartmill T. D. I. 1982; Immuno-chemistry of the cell-surface carbohydrate antigens of Clostridium difficile. Journal of General Microbiology 128:1365–1370
    [Google Scholar]
  13. Smith L. D. S. 1977; The Organism. In Botulism. The Organisms, its Toxins, the Disease pp. 15–33 Springfield, III., USA: Thomas;
    [Google Scholar]
  14. Towbin H., Staehlin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  15. Tsai C.-M., Frasch C. E. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Analytical Biochemistry 119:115–119
    [Google Scholar]
  16. Walker P. D., Batty I. 1964; Fluorescent studies in the genus Clostridium. II. A rapid method for differentiating Clostridium botulinum types A, B and F, types C and D, and type E. Journal of Applied Bacteriology 27:140–142
    [Google Scholar]
  17. Willis A. T. 1977 Anaerobic Bacteriology: Clinical and Laboratory Practice, 3rd edn. London: Butterworths;
    [Google Scholar]
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