1887

Abstract

Two isoenzymes of glutamate dehydrogenase located in the cytoplasmic fraction of were specific for NAD and NADP, respectively. The NAD-dependent enzyme functioned in both directions, i.e. amination and deamination, whereas the NADP enzyme was primarily for the amination of 2-oxoglutarate to glutamate. The NADP enzyme was purified 52-fold (free of the NAD enzyme) by affinity chromatography on 2′,5′-ADP Sepharose 4B, and some of its properties studied. Substrate activation of the amination reaction of the NADP enzyme was observed with NH and NADPH. A comparison is made of the properties of the purified NADP enzyme from and . The possible roles of two isoenzymes of glutamate dehydrogenase in are discussed.

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/content/journal/micro/10.1099/00221287-130-4-967
1984-04-01
2024-12-07
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References

  1. Aleem M. I. H. 1968; Mechanism of oxidative phosphorylation in chemoautotroph Nitrobacter agilis. Biochimica et biophysica acta 162:338–347
    [Google Scholar]
  2. Ballantine R. 1957; Determination of total nitrogen and ammonia. Methods in Enzymology 3:984–995
    [Google Scholar]
  3. Bhandari B., Nicholas D. J. D. 1979; Ammonia and O2 uptake in relation to proton translocation in cells of Nitrosomonas europaea. Archives of Microbiology 122:249–255
    [Google Scholar]
  4. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Cleland W. W. 1970; Steady state kinetics. In The Enzymes II pp. 1–61 Boyer P. D. Edited by New York: Academic Press.;
    [Google Scholar]
  6. Davis B. 1964; Disc electrophoresis II.Method and application to human serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  7. Hooper A. B., Hansen J., Bell R. 1967; Characterization of glutamate dehydrogenase from the ammonia oxidising chemoautotroph Nitrosomonas europaea. Journal of Biological Chemistry 242:288–296
    [Google Scholar]
  8. Johnson W. H., Westlake D. W. S. 1972; Purification and characterization of glutamic acid dehydrogenase and α-ketoglutaric acid reductasefromPeptococcus aerogenes. Canadian Journal of Microbiology 18:881–892
    [Google Scholar]
  9. Kew O. M., Woolfolk C. A. 1970; Preparation of glutamate dehydrogenase from Micrococcus aerogenes. Biochemical and Biophysical Research Communications 39:1126–1133
    [Google Scholar]
  10. Kramer J. 1970; NAD- and NADP-dependent glutamate dehydrogenase in Hydrogenomonas H16. Archiv für Mikrobiologie 71:226–234
    [Google Scholar]
  11. Kumar S., Nicholas D. J. D. 1981; Oxygen-dependent nitrite uptake and nitrate production by cells, spheroplasts and membrane vesicles of Nitrobacter agilis. FEMS Microbiology Letters 11:201–206
    [Google Scholar]
  12. Kumar S., Nicholas D. J. D. 1982; Assimilation of inorganic nitrogen compounds by Nitrobacter agilis. Journal of General Microbiology 128:1795–1801
    [Google Scholar]
  13. Kumar S., Nicholas D. J. D. 1984; Purification, properties and regulation of glutamine synthetase from Nitrobacter agilis. Journal of General Microbiology 130:959–966
    [Google Scholar]
  14. Le’John H. B., Suzuki I., Wright J. A. 1968; Glutamate dehydrogenases of Thiobacillus novellus.Kinetic properties and a possible control mechanism. Journal of Biological Chemistry 243:118–128
    [Google Scholar]
  15. Smith E. L., Austin B. M., Blumenthal K. M., Nyc J. F. 1975; Glutamate dehydrogenases. In The Enzymes XI pp. 293–367 Boyer P. D. Edited by New York: Academic Press.;
    [Google Scholar]
  16. Tally D. J., White R. H., Schmidt R. R. 1972; Evidence for NADH and NADPH specific isozymes of glutamate dehydrogenase and the continuous inducibility of the NADPH-specific isozyme throughout the cell cycle of eucaryotic Chlorella. Journal of Biological Chemistry 247:7927–7935
    [Google Scholar]
  17. Wallace W., Nicholas D. J. D. 1968; Properties of some reductase enzymes in the nitrifying bacteria and their relationship to the oxidase systems. Biochemical Journal 109:763–773
    [Google Scholar]
  18. Wallace W., Nicholas D. J. D. 1969; The biochemistry of nitrifying micro-organisms. Biological Reviews 44:359–391
    [Google Scholar]
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