Summary: Techniques of antigenic analysis were used to examine the synthesis of the exosporium during sporulation of . An antiserum to a soluble extract of exosporium from mature spores was used to analyse extracts of cells from various stages of the life cycle by the technique of one-dimensional immunoelectrophoresis. Exosporium antigens were absent from vegetative cell extracts and were first detected in extracts of cells at stage III of sporulation, where they appeared simultaneously in the soluble and particulate cellular fractions. The results indicate that the exosporium contains antigens which are spore specific.


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