1887

Abstract

The mycolic acid derivatives of 11 unidentified pathogenic coryneform bacteria were examined by TLC, GLC and GLC-mass spectrometry. The resulting mycolic acid profiles of the unidentified isolates were compared with those of type or reference strains of possibly related coryneform species, namely and It was apparent that most of the unidentified strains showed a distinctive mycolic acid profile, with predominant amounts of relatively high molecular weight mycolic acids (C-C) and a high degree of unsaturation, and could thus be distinguished from both , which had exceptionally low molecular weight mycolic acids (C-C), and (C-C), which had large amounts of saturated mycolic acids. The mycolates of and (C-C) were generally similar to those of the unidentified coryneforms but their overall mycolic acid patterns were different from one another as well as the unidentified strains. The mycolic acid profiles exhibited by the pathogenic coryneforms examined here were very similar to one another but unlike that of any of the type or reference strains included in the study.

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1984-03-01
2021-08-05
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References

  1. Applebaum P. C., Dossett J. H. 1981; Arthritis caused by unidentified Gram positive rods in a child with acute lymphocytic anaemia. Journal of Clinical Microbiology 15:525–527
    [Google Scholar]
  2. Becker B., Lechevalier M. P., Lechevalier H. A. 1965; Chemical composition of cell wall preparations from strains of various form-genera of aerobic actinomycetes. Applied Microbiology 13:236–243
    [Google Scholar]
  3. Collins M. D., Goodfellow M., Minnikin D. E. 1982; A survey of structures of mycolic acids in Corynebacterium and related taxa. Journal of General Microbiology 128:129–149
    [Google Scholar]
  4. Corey E. J., Venkateswarlu A. 1972; Protection of hydroxyl groups as tertiary-butyldimethylsilyl derivatives. Journal of the American Chemical Society 94:6190–6191
    [Google Scholar]
  5. Corina D. L., Sesardic D. 1980; Profile analysis of total mycolic acids from skin corynebacteria and from named Corynebacterium species by gas-liquid chromatography and gas-liquid chromatography/ mass spectrometry. Journal of General Microbiology 116:61–68
    [Google Scholar]
  6. Gailley C., Sandra P., Verzele M., Cocrro C. 1982; Analysis of mycolic acids from a group of corynebacteria by capillary gas chromatography and mass spectrometry. European Journal of Biochemistry 125:83–94
    [Google Scholar]
  7. Gill V. J., Manning C., Lamson M., Woltering P., Pizzo P. A. 1981; Antibiotic resistant group JK bacteria in hospitals. Journal of Clinical Microbiology 13:472–477
    [Google Scholar]
  8. Goldfine H. 1972; Comparative aspects of bacterial lipids. Advances in Microbial Physiology 8:1–58
    [Google Scholar]
  9. Goodfellow M., Minnikin D. E. 1977; Nocar-dioform bacteria. Annual Review of Microbiology 31:159–180
    [Google Scholar]
  10. Goodfellow M., Weaver C. H., Minnikin D. E. 1982; Numerical classification of some rhodococci, corynebacteria and related organisms. Journal of General Microbiology 128:731–745
    [Google Scholar]
  11. Goodfellow M., Beckham A. C., Barton M. D. 1983; Numerical classification of Rhodococcus equi and related actinomycetes. Journal of Applied Bac-teriology 53:199–207
    [Google Scholar]
  12. Gronemeyer P. S., Weissfeld A. C., Sonnen-Wirth A. C. 1980; Corynebacterium group JK bacterial infection in a patient with an epicardial pacemaker. American Journal of Clinical Pathology 74:838–842
    [Google Scholar]
  13. Hande K., Witebsky F., Brown M. S., Schulman C. S., Anderson S. E., Levine A. S., Mclowry J. D., Chabner B. A. 1976; Sepsis with a new species of Corynebacterium . Annals of Internal Medicine 85:423–426
    [Google Scholar]
  14. Hill L. R., Lapage S. P., Bowie I. S. 1978; Computer assisted identification of coryneform bacteria. In Coryneform Bacteria pp. 181–215 Bousfield I. J., Callely A. G. Edited by London: Academic Press;
    [Google Scholar]
  15. Hine J. L., Hill L. R., Lapage S. P. 1978; Corynebacterium spp. in human disease. Lancet ii:376
    [Google Scholar]
  16. Johnson W. D., Kaye D. 1970; Serious infections caused by diphtheroids. Annals of the New York Academy of Sciences 174:568–576
    [Google Scholar]
  17. Kaplan K., Weinstein L. 1969; Diphtheroid infections in man. Armais of Internal Medicine 70:919–929
    [Google Scholar]
  18. Lechevalier M. P. 1977; Lipids in bacterial taxonomy - a taxonomist’s viewpoint. CRC Critical Reviews in Microbiology 5:109–210
    [Google Scholar]
  19. Lechevalier M. P., Horan A. C., Lechevalier H. A. 1971; Lipid composition in the classification of nocardia and mycobacteria. Journal of Bacteriology 105:313–318
    [Google Scholar]
  20. Minnikin D. E., Goodfellow M. 1976; Lipid composition in the classification and identification of nocardiae and related taxa. In The Biology of the Nocardiae pp. 160–219 Goodfellow M., Brownell G. H., Serrano J. A. Edited by London: Academic Press;
    [Google Scholar]
  21. Minnikin D. E., Goodfellow M. 1980; Lipid composition in the classification and identification of acid-fast bacteria. In Microbiological Classification and Identification pp. 189–256 Goodfellow M., Board R. G. Edited by London: Academic Press;
    [Google Scholar]
  22. Minnikin D. E., Goodfellow M., Collins M. D. 1978; Lipid composition in the classification and identification of coryneform bacteria. In Coryneform Bacteria pp. 85–165 Bousfield I. J., Callely A. G. Edited by London: Academic Press;
    [Google Scholar]
  23. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin layer chromatography of methanolysates of mycolic acid containing genera. Journal of Chromatography 188:221–233
    [Google Scholar]
  24. Murray B. E., Krachmer A. W., Mollering R. C. 1980; Diphtheroid prosthetic valve endocarditis: a study of clinical features and infective organisms. American Journal of Medicine 69:838–848
    [Google Scholar]
  25. Riley P. S., Hollis D. G., Utter G. B., Weaver R. E., Baker C. N. 1979; Characterization of 95 diphtheroid (JK) cultures isolated from clinical specimens. Journal of Clinical Microbiology 9:418–427
    [Google Scholar]
  26. Shaw N. 1974; Lipid composition as a guide to the classification of bacteria. Advances in Applied Microbiology 17:63–108
    [Google Scholar]
  27. Stamm W. F., Tompkins L. S., Wagner K. F., Counts G. W., Thomas E. D., Myers J. D. 1979; Infections due to Corynebacterium species in marrow transplant patients. Annals of Internal Medicine 91:163–173
    [Google Scholar]
  28. Tomiyasu I. 1982; Mycolic acid composition and thermally adaptive changes in Nocardia asteroides . Journal of Bacteriology 151:828–837
    [Google Scholar]
  29. Van Scoy R. E., Cohen S. N., Geraci J. E., Washington J. A. 1977; Coryneform bacterial endocarditis. Mayo Clinical Proceedings 52:216–219
    [Google Scholar]
  30. Yano I., Saito K., Furukawa Y. 1972; Gas chromatographic and mass spectro- metric analysis of molecular species of corynemycolic acids from Corynebacterium ulcerans . FEBS Letters 21:215–219
    [Google Scholar]
  31. Yano I., Kageyama K., Ohno Y., Masui M., Kusanose E., Kusunose M., Akimori N. 1978; Separation and analysis of molecular species of mycolic acids in Nocardia and related taxa by gas chromatography and mass spectrometry. Biomedical Mass Spectrometry 5:14–24
    [Google Scholar]
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