1887

Abstract

The soluble and membrane-bound cytochromes of methylotrophic bacteria were investigated in order to determine their relationships with each other, and their roles in electron transport. The proportions of soluble cytochromes c and c in various methylotrophs were not markedly dependent on the type of methylotroph nor on conditions of growth. Between 30 and 50% of the cytochrome of Methylophilus methqdotrophus was bound tightly to membranes; 8% of this was cytochrome c, 37% was cytochrome cand 55% was the cytochrome component of the oxidase, cytochrome co. These cytochromes were purified and characterized. It was concluded that cytochrome c has no role in the membrane. By contrast, the membrane cytochrome c has a separate role from that of soluble cytochrome c; the membrane-bound cytochrome c may play a role analogous to that of mitochondria1 cytochrome c in mediating between cytochrome and the terminal oxidase during the oxidation of NADH.

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1984-12-01
2024-11-13
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References

  1. Anthony C. 1982italic The Biochemistry of Methylo- trophs. London: Academic press;
    [Google Scholar]
  2. Beardmore-Gray M. 1982italic Cytochrome c: its role in methylotrophic bacteria. PhD thesis University of Southampton, UK:
    [Google Scholar]
  3. Beardmore-Gray M., Anthony C. 1984; The methanol: cytochrome coxidoreductase activity of methylotrophs. In Microbial Growth on C1-compounds pp. 97–105
    [Google Scholar]
  4. Beardmore-Gray M., O’Keeffe D.T., Anthony C. 1982; The autoreducible cytochromes c of the methylotrophsMethylophilusmethylotrophus and PseudomonasAMI. Biochemical Journal 207:161–165
    [Google Scholar]
  5. Beardmore-Gray M., O’Keeffe D.T., Anthony C. 1983; The methanol: cytochrome coxidoreductase activity of methylotrophs. Journal of General Microbiology 129:923–933
    [Google Scholar]
  6. Burton S.M., Byrom D., Carver M., Jones G.D.D., Jones C.W. 1983; The oxidation of methylated amines by the methylotrophic bacterium Methylophilusmethylotrophus. FEMS Microbiology Letters 17:185–190
    [Google Scholar]
  7. Carver M.A., Jones C.W. 1983; The terminal respiratory chain of the methylotrophic bacterium, Methylophilusmethylotrophus. FEBS Letters 155:187–191
    [Google Scholar]
  8. Carver M.A., Jones C.W. 1984; The role of c- type cytochromes in the terminal respiratory chain of the methylotrophic bacterium Methylophilusmethylotrophus. Archives of Microbiology 139:76–82
    [Google Scholar]
  9. Cross A.R. 1980italic Electron transport in Methylophilusmethylotrophus. PhD thesis University of Southampton, UK.:
    [Google Scholar]
  10. Cross A.R., Anihony C. 1980; The electron transport chains of the obligate methylotrophMethylophilusmethylotrophus. Biochemical Journal 192:429–439
    [Google Scholar]
  11. Dawson M.J., Jones C.W. 1981; Energy conservation in the terminal region of the respiratory chain of the methylotrophic bacterium Methylophilusmethylotrophus. European Journal of Biochemistry 118:113–118
    [Google Scholar]
  12. Froud S.J., Anthony C. 1984; The purification and characterization of the o-type cytochrome oxidase from Methylophilusmethylotrophus, and its reconstitution into a ‘methanol oxidase’ electron transport chain. Journal of General Microbiology 130:2201–2212
    [Google Scholar]
  13. Jones C.W., Kingsbury S.A., Dawson M.J. 1982; The partial resolution and dye-mediated reconstitution of methanol oxidase activity in Methylophilusmethylotrophus. FEMS Microbiology letters 13:195–200
    [Google Scholar]
  14. Maclennen D.G., Ousby J.C., Vasey R.B. 1971; The influence of dissolved oxygen on Pseudomonas AMI grown on methanol in continuous culture. Journal of General Microbiology 69:395–404
    [Google Scholar]
  15. Ohta S., Tobari J. 1981; Two cytochromes c of Methylomonas J. Journal of Biochemistry 90:215–224
    [Google Scholar]
  16. Van Verseveld H.W., Stouthamer A.H. 1978; Electron-transport chain and coupled oxidative phosphorylation in methanol-grown Paracoccusdenitrificans. Archives of Microbiology 118:13–20
    [Google Scholar]
  17. Vrdoljak M., Froud S.J. 1982; Characterisation of the electron transport chain of an obligate methylotroph strain 4025. International Journal of Biochemistry 14:1019–1025
    [Google Scholar]
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