1887

Abstract

The soluble and membrane-bound cytochromes of methylotrophic bacteria were investigated in order to determine their relationships with each other, and their roles in electron transport. The proportions of soluble cytochromes c and c in various methylotrophs were not markedly dependent on the type of methylotroph nor on conditions of growth. Between 30 and 50% of the cytochrome of Methylophilus methqdotrophus was bound tightly to membranes; 8% of this was cytochrome c, 37% was cytochrome cand 55% was the cytochrome component of the oxidase, cytochrome co. These cytochromes were purified and characterized. It was concluded that cytochrome c has no role in the membrane. By contrast, the membrane cytochrome c has a separate role from that of soluble cytochrome c; the membrane-bound cytochrome c may play a role analogous to that of mitochondria1 cytochrome c in mediating between cytochrome and the terminal oxidase during the oxidation of NADH.

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1984-12-01
2021-10-25
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