1887

Abstract

2-Deoxy--glucose added to cultures of in the stationary phase of growth inhibited the incorporation of glucose into the (1→3)--glucan fraction of the organisms. In the presence of ATP and cell extracts it was converted to 2-deoxy--glucose phosphate and when UTP was also present, material with the electrophoretic properties of UDP-2-deoxy--glucose was formed. In similar conditions glucose formed glucose phosphates, UDP-glucose and other products. Evidence was obtained that the analogue, after conversion to a phosphate derivative, was an inhibitor of phosphoglucomutase.

When was grown in the presence of 2-deoxy--glucose for 24 h, analogue residues became incorporated into the (1→3)--glucan fraction and the subsequent rate of incorporation of glucose into that fraction was enhanced. The rate of turnover of glucose in this -glucan fraction was greater than in controls. Pretreatment of cultures with -glucanase, or incubation under conditions known to stimulate endogenous -glucanases, increased the subsequent rate of glucose incorporation and this increase was enhanced by growth in the presence of 2-deoxy--glucose. The analogue thus had the effect of altering the stability and glucose-acceptor function of (1→3)--glucan chains. This could affect the properties of the polymer network leading to the known effect of the analogue in delaying the onset of phenotypic resistance to amphotericin methyl ester in stationary phase cultures of

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1984-12-01
2024-04-26
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References

  1. Bieleski R.L., Young R.E. 1963; Extraction and separation of phosphate esters from plant tissues. Analytical Biochemistry 6:54–68
    [Google Scholar]
  2. Biely P., Kratky Z., Bauer S. 1972; Metabolism of 2-deoxyglucose by bakers’ yeast. IV. Incorporation of 2-deoxyglucose into cell wall mannan. Biochimica et biophysica acta 255:631–639
    [Google Scholar]
  3. Biely P., Kratky Z., Bauer S. 1974; Metabolism of 2-deoxyglucose by bakers’ yeast. VI. A study on cell wall mannan. Biochimica et biophysica acta 352:268–274
    [Google Scholar]
  4. Cassone A., Kerridge D., Gale E.F. 1979; Ultrastructural changes in the cell wall of Candida albicans following cessation of growth and their possible relationship to the development of polyene resistance. Journal of General Microbiology 110:339–349
    [Google Scholar]
  5. Farkas V. 1979; Biosynthesis of cell walls of fungi. Microbiological Reviews 43:117–144
    [Google Scholar]
  6. Gale E.F. 1974; The release of potassium ions from Candida albicans in the presence of polyene antibiotics. Journal of General Microbiology 80:451–465
    [Google Scholar]
  7. Gale E.F., Johnson A.M., Kerridge D., Miles E.A. 1978; Phenotypic resistance to amphotericin B in Candida albicans: the role of reduction. Journal of General Microbiology 109:191–204
    [Google Scholar]
  8. Gale E.F., Ingram J., Kerridge D., Notario V., Wayman F. 1980; Reduction of amphotericin resistance in stationary phase cultures of Candida albicans by treatment with enzymes. Journal of General Microbiology 117:383–391
    [Google Scholar]
  9. Johnson B.F. 1968; Lysis of yeast cell walls induced by 2 deoxy-glucose at their sites of glucan synthesis. Journal of Bacteriology 95:1169–1174
    [Google Scholar]
  10. Notario V. 1982; βGlucanases from Candida albicans: purification, characterization and the nature of their attachment to cell wall components. Journal of General Microbiology 128:747–759
    [Google Scholar]
  11. Notario V., Gale E.F., Kerridge D., Wayman F. 1982; Phenotypic resistance to amphotericin B in Candida albicans: relationship to glucan metabolism. Journal of General Microbiology 128:761–111
    [Google Scholar]
  12. Orlean P.A.B. 1982; (1→3)-β-Glucan synthase from budding and filamentous cultures of the dimorphic fungus Candida albicans. European Journal of Biochemistry 127:397–403
    [Google Scholar]
  13. Orlean P.A.B., Kerridge D. 1981; (l→3)-β-Glucan and glycogen synthase activities from budding and filamentous cultures of Candida albicans. In Abstracts of VIIth International Symposium on Yeast p. 92 Valencia, Spain;
    [Google Scholar]
  14. Orlean P.A.B., Ward S.M. 1983; Sodium fluoride stimulates (1→3)- β-glucan synthase from Candida albicans. FEMS Microbiology Letters 18:31–35
    [Google Scholar]
  15. Schwarz R.T., Datema R. 1982; Inhibition of lipid-dependent glycosylation. In The Glycoconjugates III pp. 47–49
    [Google Scholar]
  16. Villanueva J.R., Notario V., Santos T., Villa T.G. 1976; βGlucanases in nature. Biochemistry and function of βglucanases in yeast. In Microbial and Plant Protoplasts pp. 323–355 Edited by Peberdy J. F., Rose A. H., Cocking E. C. London: Academic Press;
    [Google Scholar]
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