1887

Abstract

Purified mitochondria were obtained from the phytopathogenic fungus f. sp. by mechanical disruption of protoplasts, followed by differential and density gradient centrifugation. DNA, extracted from the mitochondria, was shown by electron microscopy and restriction endonuclease analysis to be a 46·5 kilobase pair circular molecule.

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1984-11-01
2024-04-26
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References

  1. Bernard U., Bade E., Kuntzel H. 1975; Specific fragmentation of mitochondrial DNA from Neurospora crassa by restriction endonuclease EcoRl. Biochemical and Biophysical Research Communications 64:783–789
    [Google Scholar]
  2. Clark-Walker G.D., Sriprakash K.S. 1981; Sequence rearrangements between mitochondrial DN As of Torulopsis glabrata and Kloeckera africana. Journal of Molecular Biology 151:367–387
    [Google Scholar]
  3. Guardiola J., Grimaldi G., Constantino P., Micheli G., Cervone F. 1982; Loss of nitrofuran resistance in Fusarium ~xysporum is correlated with loss of a 46·7 kb circular DNA molecule. Journal of General Microbiology 128:2235–2242
    [Google Scholar]
  4. Ifft J.B., Voet D.H, Vinoorad J. 1961; The determination of density distributions and density gradients in binary solutions at equilibrium in the ultracentrifuge. Journal of Physical Chemistry 65:1138–1145
    [Google Scholar]
  5. Kleinschmidt A.K., Lang D., Jackbrts D, Zahn R.K. 1962; Darstellung und Lfingenmes- sungen des gesamten Desoxyribonucteinsfiurc-inhaltes von T2-Bakteriophagcn. Biochimica et bio-physica acta 61:857–864
    [Google Scholar]
  6. Kück U., Stahl U, Esser K. 1981; Plasmid-like DNA is part of mitochondria] DNA in Podospora anserina. Current Genetics 3:151–156
    [Google Scholar]
  7. Kück U., Stahl U., Lhermitte A, Esser K. 1980; Isolation and characterisation of mitochondrial DNA from the alkane yeast Saccharomycopsis Hpolytica. Current Genetics 2:97–101
    [Google Scholar]
  8. Lazarus C.M., Earl A.J., Turner G, Küntzbl H. 1980; Amplification of a mitochondrial DNA sequence in the cytoplasmically inherited ‘ragged’ mutant of Aspergillus amstelodami. European Journal of Biochemistry 106:633–641
    [Google Scholar]
  9. Locker I., Lewin A., Rabinowitz M. 1979; The structure and organisation of mitochondrial DNA from petite yeast. Plasmid 2:155–181
    [Google Scholar]
  10. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual: Cold Spring Harbor, NY;
  11. Minuth W., Tudzynski P, Esser K. 1982; Extrachromosoma) genetics of Cephalosporium acre- monium. I. Characterisation and mapping of mitochondrial DNA. Current Genetics 5:227–231
    [Google Scholar]
  12. Rolfe R, Meselson M. 1959; The relative homogeneity of microbial DNA. Proceedings of the National Academy of Sciences of the United States of America 45:1039–1043
    [Google Scholar]
  13. Sanger F., Coulson A.R., Hong G.F., Hill D.F, Petersen G.B. 1982; Nucleotide sequence of bacteriophage λ DNA. Journal of Molecular Biology 162:729–773
    [Google Scholar]
  14. Schnarrenberg C., Deser A., Tolbert N.E. 1971; Development of microbodies in sunflower cotyledons and castor bean endosperm during germination. Plant Physiology 48:566–574
    [Google Scholar]
  15. Stepein P.P., Bernard U., Cooke H.J, Küntzel H. 1978; Restriction endonuclease cleavage map of mitochondrial DNA from Aspergillus nidulans. Nucleic Acids Research 5:317–330
    [Google Scholar]
  16. Tudzynski P., Esser K. 1979; Chromosomal and extrachromosomal control of senescence in the ascomycete Podospora anserina. Molecular and General Genetics 173:71–84
    [Google Scholar]
  17. Twigg A.J., Sherratt D. 1980; Trans-comple- mentable copy-number mutants of plasmid Col El. London: Nature; 283216–218
    [Google Scholar]
  18. Van Heuverswyn H., Fibrs W. 1979; Nucleotide sequence of the Hind C fragment of simian virus 40 DNA. European Journal of Biochemistry 100:51–60
    [Google Scholar]
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