1887

Abstract

Plasmid formation by derivatives of lambdoid phages has been reinvestigated with transducing phages carrying the and genes of Transduction by λ I derivatives was inefficient and short-lived in each case, under both selective and nonselective conditions. Mutant operators were introduced to relieve possible auto-repression by the gene product. Such -defective phage genomes were able to propagate continually as plasmids, although without selection they were gradually lost from the carrier cells. Plasmid formation remained inefficient, however. The entire chromosome of phages can be expressed by transcription that leaks through the serially arranged Rho-dependent terminators. Some functions so expressed are deleterious to the plasmid state and cause the instability of λ plasmids.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-10-2501
1984-10-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/10/mic-130-10-2501.html?itemId=/content/journal/micro/10.1099/00221287-130-10-2501&mimeType=html&fmt=ahah

References

  1. Adhya S., Sen A., Mitra A. 1971; The role of gene S. In The Bacteriophage Lambda pp. 743–746 Hershey A.D. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  2. Anilonis A. 1977 A restriction map of Escherichia coli trp operon DNA. PhD thesis. University of Edinburgh;
    [Google Scholar]
  3. Appleyard R.K. 1954; Segregation of new lysogenic types during growth of a doubly lysogenic-strain derived f rom Escherichia coli K 12. Genetics 39:440–452
    [Google Scholar]
  4. Beckwith J.R. 1978; lac: the genetic system. In The Operon. pp. 11–30 Miller J.H., Reznikoff W.S. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Benchimo S., Lucko H., Becker A. 1982; A novel endonuclease specified by bacteriophage λ. Journal of Biological Chemistry 257:5211–5219
    [Google Scholar]
  6. Benedik M., Mascarenhas D., Campbell A. 1983; The integrase promoter and Tj terminator of bacteriophage λ and 434. Virology 126:658–668
    [Google Scholar]
  7. Blattner F.R., Fiandt M., Hass K.K., Twose P.A., Szybalski W. 1974; Deletions and insertions in the immunity region of coliphage lambda: revised measurement of the promoter-startpoint distance. Virology 62:458–471
    [Google Scholar]
  8. Brammar W.J., Murray N.E., Winston S. 1974; Restriction of trp bacteriophages by Escherichia coli K. Journal of Molecular Biology 90:633–647
    [Google Scholar]
  9. Burt D. 1981 Gene-fusion and the analysis of genetic control signals. PhD thesis. University of Leicester;
    [Google Scholar]
  10. Campbell A. 1961; Sensitive mutants of bacteriophage λ. Virology 14:22–32
    [Google Scholar]
  11. Campbell A., Babinder E. 1958; Properties of transducing phages. Yearbook of the Carnegie Institute, Washington pp. 386–389
    [Google Scholar]
  12. Clark A.J. 1967; The beginning of a genetic analysis of recombination proficiency. Journal of Cell Physiology 70: Supplement I 165–180
    [Google Scholar]
  13. Court D., Campell A. 1972; Gene regulation in N mutants of bacteriophage X. Journal of Virology 9:938–945
    [Google Scholar]
  14. Davis R.W, Bostein D., Roth J.R. 1980 Adianced Bacterial Genetics pp. 70–77 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Dennert G., Henning V. 1968; Tyrosine incorporating amber suppressors in Escherichia coli K12. Journal of Molecular Biology 33:327–329
    [Google Scholar]
  16. Drahos D., Szybalski W. 1981; Antitermination and termination functions of the cloned nuiL, N and /Ll modules of coliphage lambda. Gene 16:261–274
    [Google Scholar]
  17. Eisen H.A., Fuerst C.R., Siminovitch L., Thomas R., Lambert L., Pereira DA SILVA L., Jacob F. 1966; Genetics and physiology of defective lysogeny in K12 (λ): studies in early mutants. Virology 30:224–241
    [Google Scholar]
  18. Enquist L.W., Skalka A. 1973; Replication of bacteriophage A DNA dependent on the function of host and viral genes. Journal of Molecular Biology 75:185–212
    [Google Scholar]
  19. Feiss M., Becker A. 1983; DNA packaging and cutting. In Lambda II pp. 305–330 Hendrix R., Roberts J., Stahl F., Weisberg R. Edited by New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  20. Feiss M., Adhya S., Court D. 1972; Isolation of plaque-forming galactose-transducing strains of phage lambda. Genetics 71:189–206
    [Google Scholar]
  21. Franklin N.C. 1967; Deletions and functions of the center of the ^80-lphage genome. Evidence for a phage function promoting genetic recombination. Genetics 57:301–318
    [Google Scholar]
  22. Franklin N.C. 1971; The N operon of lambda: extent and regulation as observed in fusions to the tryptophan operon of Escherichia coli. In The Bacteriophage Lambda pp. 621–638 Hershey A.D. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Garrett J., Fusselman R., Hise J., Chiou L., Smith-GRILLO D., Schulz J., Young R. 1981; Cell lysis by induction of cloned lambda lysis genes. Molecular and General Genetics 182:326–331
    [Google Scholar]
  24. Goldberg A.R., Howe M. 1969; New mutations in the S cistron of bacteriophage lambda affecting host cell lysis. Virology 38:200–210
    [Google Scholar]
  25. Greenstein M., Skalka A. 1975; Replication of bacteriophage lambda DNA: in vivo studies of the interaction between the viral gamma protein and the host recBC DNase. Journal of Molecular Biology 97:543–559
    [Google Scholar]
  26. Hadfield C., Brammar W.J. 1984; Functions antagonistic to plasmid formation by λN chromosomes. Journal of General Microbiology 130:2515–2526
    [Google Scholar]
  27. Herskowitz I. 1973; Control of gene expression in bacteriophage lambda. Annual Review of Genetics 7:289–324
    [Google Scholar]
  28. Herskowitz I., Hagen D. 1980; The lysis lysogeny decision of phage explicit programming and responsiveness. Annual Review of Genetics 14:399–445
    [Google Scholar]
  29. Hobom G., Hogness D.S. 1974; The role of recombination in the formation of circular oligomers of the ,λdv I plasmid. Journal of Molecular Biology 88:65–87
    [Google Scholar]
  30. Hopkins N., Ptashne M. 1971; Genetics of virulence. In The Bacteriophage Lambda pp. 571–588 Hershey A.D. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Hopkins A.S., Murray N.E., Brammar W.J. 1976; Characterization of λrrp-transducing bacteriophages made in vitro. Journal of Molecular Biology 17:549–569
    [Google Scholar]
  32. Ippen K., Shapiro J.A., Beckwith J.R. 1971; Transposition of the lac region to the gal region of the Escherichia coli chromosome: isolation of the Xlac transducing bacteriophages. Journal of Bacteriology 108:5–9
    [Google Scholar]
  33. Jacob F., Wollman E.L. 1954; Etude genetique d'un bacteriophage tempere d’Escherichia coli. I. Le systeme genetique du bacteriophage. Annates de 1’Institut Pasteur 87:653–690
    [Google Scholar]
  34. Joyner A., Isaacs L.N., Echols H., Sly W.S. 1966; DNA replication and messenger RNA production after induction of wild type λ bacteriophage and mutants. Journal of Molecular Biology 19:174–186
    [Google Scholar]
  35. Kaiser A.D., Jacob F. 1957; Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localisation. Virology 4:509–521
    [Google Scholar]
  36. Kleckner N., Signer E. 1977; Genetic characterization of plasmid formation by λ mutants of bacteriophage λ. Virology 79:160–173
    [Google Scholar]
  37. Lennox E.S. 1955; Transduction of linked genetic characters of the host by bacteriophage PI. Virology 1:190–206
    [Google Scholar]
  38. Lleb M. 1970; λ mutants which persist as plasmids. Journal of Virology 6:218–225
    [Google Scholar]
  39. Lieb M. 1971; Lysogenization of lambda lacking repressor and N function. In The Bacteriophage Lambda pp. 679–690 Hershey A.D. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  40. Liedke-KULKE M., Kaiser A.D. 1967; The c-region of coliphage 21. Virology 32:475–481
    [Google Scholar]
  41. Luk K.-C., Szybalski W. 1982; Transcription termination: sequence and function of the rho-dependent i terminator in the major leftward operon of bacteriophage lambda. Gene 17:247–258
    [Google Scholar]
  42. Luk K.-C., Szybalski W. 1983a; The r1,2 cluster of transcription termination sites between genes bet and rat of coliphage lambda. Virology 125:403–418
    [Google Scholar]
  43. Luk K.-C, Szybalski W. 1983b; A cluster of leftward. rAo-dependent t, terminators in the J gene of coliphage lambda. Gene 21:175–191
    [Google Scholar]
  44. Luk K.-C., Dobrzanski P., Slszybalski W. 1982; Cloning and characterization of the termination site r, for the gene int transcript of phage lambda. Gene 17:259–262
    [Google Scholar]
  45. Malamy MH., Fiandt M., Szybalski W. 1972; Electron microscopy of polar insertions in the lac operon of Escherichia coli. Molecular and General Genetics 119:207–222
    [Google Scholar]
  46. Matsubara K. 1974; Interference in bacteriophage growth by a resident plasmid Adv. II. Role of the promoter-operator. Journal of Virology 13:603–607
    [Google Scholar]
  47. Moir A., Brammar W.J. 1976; The use of specialised transducing phages in the amplification of enzyme production. Molecular and General Genetics 149:87–99
    [Google Scholar]
  48. Murray N.E., Brammar W.J. 1973; The trpE gene of Escherichia coli K contains a recognition sequence for the K-restriction system. Journal of Molecular Biology 77:615–624
    [Google Scholar]
  49. Murray N.E., Batten P.L., Murray K. 1973; Restriction of bacteriophage k by Escherichia coli K. Journal of Molecular Biology 81:395–407
    [Google Scholar]
  50. Ogawa T., Tomizawa J. 1968; Replication of bacteriophage DNA. I. Replication of DNA of lambda phage defective in early functions. Journal of Molecular Biology 38:217–226
    [Google Scholar]
  51. Parkinson J.S. 1968; Genetics of the left arm of the chromosome of bacteriophage lambda. Genetics 59:311–325
    [Google Scholar]
  52. Reader R.W., Siminovitch L. 1971a; Lysis defective mutants of bacteriophage lambda: genetics and physiology of 5 cistron mutants. Virology 43:607–622
    [Google Scholar]
  53. Reader R.W., Siminovitch L. 1971b; Lysis defective mutants of bacteriophage lambda: on the role of the S function in lysis. Virology 43:623–637
    [Google Scholar]
  54. Roades R.W., Siminovitch L. 1973; An endonuclease induced by bacteriophage. Journal of Biological Chemistry 248:521–527
    [Google Scholar]
  55. Rosenberg M., Court D., Shimatake H., Brady C., Wulff D.L. 1978; The relationship between functions and DNA sequence in an intercistronic regulatory region in phage λ. Nature: London; 272:414–423
    [Google Scholar]
  56. Salstrom J.S., Szybalski W. 1978; Transcription termination sites in the major leftward operon of coliphage lambda. Virology 88:252–262
    [Google Scholar]
  57. Signer E.R. 1969; Plasmid formation: a new mode of lysogeny by phage λ. Nature: London; 223158–160
    [Google Scholar]
  58. Signer E. 1971; General recombination. In The Bacteriophage Lambda pp. 139–174 Hershey A.D. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  59. Sumner-SMITH M., Benchimol S., Murialdo H., Becker A. 1982; The ben gene of bacteriophage A. Mapping, identification and control of synthesis. Journal of Molecular Biology 160:1–22
    [Google Scholar]
  60. Syvanen M. 1974; In vitro genetic recombination of bacteriophage λ. Proceedings of the National Academy of Sciences of the United States of America 71:2496–2499
    [Google Scholar]
  61. Vogel H.J., Bonner D.M. 1956; Acetylornithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  62. Weil J., Signer E.R. 1968; Recombination in bacteriophage λ. Journal of Molecular Biology 34:261–271
    [Google Scholar]
  63. Yanofsky C., Ito J. 1966; Nonsense codons and polarity in the tryptophan operon. Journal of Molecular Biology 21:313–334
    [Google Scholar]
  64. Zissler J., Signer E.R., Schaefer F. 1971; The role of recombination in growth of bacteriophage lambda. II. Inhibition of growth by prophage P2. In The Bacteriophage Lambda pp. 469–476 Hershey A.D. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-10-2501
Loading
/content/journal/micro/10.1099/00221287-130-10-2501
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error