1887

Abstract

SUMMARY: Plasmid formation by derivatives of lambdoid phages has been reinvestigated with transducing phages carrying the and genes of Transduction by λ I derivatives was inefficient and short-lived in each case, under both selective and nonselective conditions. Mutant operators were introduced to relieve possible auto-repression by the gene product. Such -defective phage genomes were able to propagate continually as plasmids, although without selection they were gradually lost from the carrier cells. Plasmid formation remained inefficient, however. The entire chromosome of phages can be expressed by transcription that leaks through the serially arranged Rho-dependent terminators. Some functions so expressed are deleterious to the plasmid state and cause the instability of λ plasmids.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-10-2501
1984-10-01
2020-10-23
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-10-2501
Loading

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error