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Inhibition by sodium azide of the growth of Bacillus coagulans decreased but the cytochrome content, particularly cytochrome d, increased with increasing growth temperature. Higher cytochrome d content correlated with increased cyanide resistance of NADH oxidase, but azide resistant activity was found in mesophilic and thermophilic cultures. Anaerobic growth at 37 °C was totally inhibited by 1 mm-azide. At 55 °C; growth occurred with 4 mm-azide, but the cell yield was reduced by 60%. ATPase activity was sensitive to azide but inhibition varied with both growth and assay temperatures. ATPase from cells grown at 55 °C; was least sensitive when assayed at 55 °C;. Possible changes in ATPase which could account for the temperature-dependent azide sensitivity are discussed.
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