SUMMARY: Inhibition by sodium azide of the growth of decreased but the cytochrome content, particularly cytochrome , increased with increasing growth temperature. Higher cytochrome content correlated with increased cyanide resistance of NADH oxidase, but azide resistant activity was found in mesophilic and thermophilic cultures. Anaerobic growth at 37°C was totally inhibited by 1 mM-azide. At 55°C; growth occurred with 4 mM-azide, but the cell yield was reduced by 60%. ATPase activity was sensitive to azide but inhibition varied with both growth and assay temperatures. ATPase from cells grown at 55°C; was least sensitive when assayed at 55°C;. Possible changes in ATPase which could account for the temperature-dependent azide sensitivity are discussed.


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