1887

Abstract

The adaptation of cells of f. sp. for growth under denitrifying conditions in the light has been studied. The presence of nitrate in photosynthetically grown bacterial cultures resulted in a drastic reduction of carotenoid and bacteriochlorophyll contents as well as a loss of one of the polypeptides of the light harvesting complex, resulting in colour changes. Denitrifying cells had high activities of nitrate, nitrite and nitrous oxide reductases. The polypeptides corresponding to subunits of these enzymes were separated by PAGE. Synthesis of these enzymes was studied by pulse-chase labelling techniques. Nitrate and nitrite reductases are constitutive enzymes and it is likely that copies of mRNA for synthesis of these enzymes are ‘long lived’ in the cells.

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1984-01-01
2021-05-17
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References

  1. Aagaard J., SistrØm W. R. 1972; Control of synthesis of reaction center bacteriochlorophyll in photosynthetic bacteria. Photochemistry and Photobiology 15:209–225
    [Google Scholar]
  2. Amesz J. 1968; Fluorescence and energy transfer. In The Photosynthetic Bacteria pp. 333–341 Clayton R. K., Sistrom W. R. Edited by New York:: Plenum.;
    [Google Scholar]
  3. Averill B. A., Tiedje J. M. 1982; The chemical mechanism of microbial denitrification. FEBS Letters 138:8–12
    [Google Scholar]
  4. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  5. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  6. Chory J., Kaplan S. 1983; Light-dependent regulation of the synthesis of soluble and intro- cytoplasmic membrane proteins of Rhodopseudomonas sphaeroides. Journal of Bacteriology 153:465–474
    [Google Scholar]
  7. Cogdell J. R., Parson W. W., Kerr M. A. 1976; The type, amount, location and energy transfer properties of the carotenoid inreaction centres from Rhodopseudomonas sphaeroides. Biochimica et biophysica acta 430:83–93
    [Google Scholar]
  8. Cohen L. K., Kaplan S. 1981; Characterization of three major intracytoplasmic membrane polypeptides isolated from Rhodopseudomonas sphaeroides. Journal of Biological Chemistry 256:5909–5915
    [Google Scholar]
  9. Cohen-Bazire G., SistrØm W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by nonsulfur purple bacteria. Journal of Cellular and Comparative Physiology 49:25–68
    [Google Scholar]
  10. Hallenbeck P. C., Meyer C. M., Vignais P. M. 1982; Regulation of nitrogenase in the photosynthetic bacterium Rhodopseudomonas capsulata as studied by two-dimensional gel electrophoresis. Journal of Bacteriology 151:1612–161
    [Google Scholar]
  11. Hillmer P., Gest H. 1975; H2 metabolism in the photosynthetic bacterium Rhodopseudomonas capsulata. H2 production by growing culture. Journal of Bacteriology 129:724–731
    [Google Scholar]
  12. Iborra F., Buchler J. M. 1976; Protein subunit mapping.A sensitive high resolution method. Analytical Biochemistry 74:503–511
    [Google Scholar]
  13. Kaplan S. 1978; Control and kinetics of photosynthetic membrane development. In The Photosynthetic Bacteria pp. 809–839 Clayton R. K., Sistrom W. R. Edited by New York:: Plenum.;
    [Google Scholar]
  14. Kaplan S. 1981; Development of the membranes of photosynthetic bacteria. Photochemistry and Photobiology 34:769–774
    [Google Scholar]
  15. Katan M. B. 1976; Detection of cytochromes on sodium dodecyl-sulphate-polyacrylamide gels by their intrinsic fluorescence. Analytical Biochemistry 74:132–137
    [Google Scholar]
  16. Kelley B. C., Dunstan R. M., Nicholas D. J. D. 1982; Respiration-dependent nitrogenase activity in the dark in a denitrifying phototrophic bacterium Rhodopseudomonas sphaeroides forma sp.denitrificans. FEMS Microbiology Letters 13:253–258
    [Google Scholar]
  17. Kerber N. L., Cardenas J. 1982; Nitrate reductase from Rhodopseudomonas sphaeroides. Journal of Bacteriology 150:1091–1097
    [Google Scholar]
  18. Klemme J-H., Chyla I., Preuss M. 1980; Dissimilatory nitrate reduction by strains of the facultative phototrophic bacterium Rhodopseudomonas palustris. FEMS Microbiology Letters 9:137–140
    [Google Scholar]
  19. Kristjansson J. K., Hollocher T. C. 1980; First practical assay for extracellular nitrous oxide reductase from denitrifying bacteria and a preliminary kinetic characterization of the soluble enzyme. Journal of Biological Chemistry 255:704–707
    [Google Scholar]
  20. Kundu B., Nicholas D. J. D. 1983; Purification and properties of a dissimilatory nitrite reductase from a phototrophic denitrifying bacterium. Proceedings of the Australian Biochemical Society 15:57
    [Google Scholar]
  21. Laemmli U. K., Favre M. 1973; Maturation of the head of bacteriophage T4. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  22. Madigan M. T., Wall J. D., Gest H. 1979; Dark anaerobic dinitrogen fixation by a photosynthetic microorganism. Science 204:1429–1430
    [Google Scholar]
  23. Matsubara T., Zumft W. G. 1982; Identification of a copper protein as part of the nitrous oxide- reducing system in nitrite-respiring (denitrifying) Pseudomonads. Archives of Microbiology 132:322–328
    [Google Scholar]
  24. Meyer T. E., Bartsch R. G., Kamen M. D. 1971; A class of electron transfer heme proteins found in both photosynthetic and sulfate-reducing bacteria. Biochimica et biophysica acta 245:453–464
    [Google Scholar]
  25. Michalski W. P., Nicholas D. J. D. 1983; Regulation of nitrogen fixation by glutamine in Rhodopseudomonassphaeroides f. sp.denitrificans. Proceedings of the Australian Biochemical Society 15:54
    [Google Scholar]
  26. Michalski W. P., Nicholas D. J. D., Vignais P. M. 1983; 14C-labelling of glutamine synthetase and Fe protein of nitrogenase in toluene-treated cells of Rhodopseudomonas capsulata. Biochimica et biophysica acta 743:136–148
    [Google Scholar]
  27. O’Farrell P. H. J. 1975; High resolution twodimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  28. Payne W. J. 1973; Reduction of nitrogenous oxides by microorganisms. Bacteriological Reviews 37:409–451
    [Google Scholar]
  29. Reed D. W. 1969; Isolation and composition of a photosynthetic reaction centre complex from Rhodopseudomonas sphaeroides. Journal Biological Chemistry 244:4936–4941
    [Google Scholar]
  30. Satoh T. 1981; Soluble dissimilatory nitrate reductase containing cytochrome c from a photodenitrifierRhodopseudomonas sphaeroides forma sp.denitrificans. Plant & Cell Physiology 22:443–452
    [Google Scholar]
  31. Satoh T., Hoshino Y., Kitamura M. 1976; Rhodopseudomonassphaeroides forma sp.denitrificans, a denitrifying strain as a subspecies of Rhodopseudomonas sphaeroides. Archives of Microbiology 108:265–269
    [Google Scholar]
  32. Sawada E., Satoh T. 1980; Periplasmic location of dissimilatory nitrate and nitrite reductases in a denitrifying phototrophic bacterium, Rhodopseudomonas sphaeroides forma sp.denitrificans. Plant & Cell Physiology 21:205–210
    [Google Scholar]
  33. Sawada E., Satoh T., Kitamura H. 1978; Purification and properties of a dissimilatory nitrite reductase of a denitrifying phototrophic bacterium. Plant & Cell Physiology 19:1339–1351
    [Google Scholar]
  34. Stanley P. E., Williams S. G. 1969; Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme. Analytical Biochemistry 29:381–392
    [Google Scholar]
  35. Stouthamer A. H. 1976; Biochemistry and genetics of nitrate reductase in bacteria. Advances in Microbial Physiology 14:315–375
    [Google Scholar]
  36. Urata K., Shimada K., Satoh T. 1982; Periplasmic location of nitrous oxide reductase in a photodenitrifier, Rhodopseudomonas sphaeroides forma sp.denitrificans. Plant & Cell Physiology 23:1121–1124
    [Google Scholar]
  37. Wehrli W., KnÜsel F., Schmid K., Staehelin M. 1968; Interaction of rifamycin with bacterial RNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 61:667–673
    [Google Scholar]
  38. Whatley F. R. 1981 Biology of Inorganic Nitrogen and Sulphur. Proceedings in Life Sciences pp. 64–77 Bothe H., Trebst A. Edited by New York & Berlin:: Springer-Verlag.;
    [Google Scholar]
  39. Wood P. M. 1981; Fluorescent gels as a general technique for characterizing bacterial c-type cytochromes. Analytical Biochemistry 111:235–239
    [Google Scholar]
  40. Zumft W. G., Matsubara T. 1982; A novel kind of multi-copper protein as terminal oxidoreductase of nitrous oxide respiration in Pseudomonas perfectomarinus. FEBS Letters 148:107–112
    [Google Scholar]
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