1887

Abstract

Summary: In the production of high titre staphylococcus phage K lysates from concentrated liquid bacterial cultures the upper limit of phage concentration appears to be at about 10 particles/ml. This concentration, the ‘ceiling titre’, can be raised to 4 × 10 particles/ml. by the addition of ammonium sulphate to the medium after phage infection. It is concluded that this effect depends upon the ability of ammonium sulphate to lower the rate of adsorption of phage upon host cells, thus preventing a high rate of phage loss by adsorption and inactivation towards the end of lysis. The possibility is discussed that this mechanism may explain some puzzling observations in lysing systems.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-13-1-185
1955-08-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/13/1/mic-13-1-185.html?itemId=/content/journal/micro/10.1099/00221287-13-1-185&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1950; Section I. Methods of study of bacterial viruses. Meth. med. Res 2:19
    [Google Scholar]
  2. Bradley P. L., Boyd J. S. K. 1952; Readsorption of phage produced in cultures of lysogenic strains of Salmonella typhi-murium. J. Path. Bact 64:891
    [Google Scholar]
  3. Delbrück M. 1945; Effects of specific anti-sera on the growth of bacterial viruses (bacteriophages). J. Bact 50:137
    [Google Scholar]
  4. Farrant J. L., Rountree P. M. 1953; Electronmicroscopy of a staphylococcal bacteriophage. J. gen. Microbiol 9:288
    [Google Scholar]
  5. French R. C., Graham A. F., Lesley S. M., Rooyen C. E. van. 1952; The contribution of phosphorus from T2r+ bacteriophage to progeny. J. Bact 64:597
    [Google Scholar]
  6. Hotchin J. E. 1954; The purification and electron microscopical examination of the structure of staphylococcal bacteriophage K. J. gen. Microbiol 10:250
    [Google Scholar]
  7. Hotchin J. E., Dawson I. M., Elford W. J. 1952; The use of empty bacterial membranes in the study of the adsorption of staphylococcus K phage upon its host. Brit. J. exp. Path 33:177
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-13-1-185
Loading
/content/journal/micro/10.1099/00221287-13-1-185
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error