Summary: In the production of high titre staphylococcus phage K lysates from concentrated liquid bacterial cultures the upper limit of phage concentration appears to be at about 10 particles/ml. This concentration, the ‘ceiling titre’, can be raised to 4 × 10 particles/ml. by the addition of ammonium sulphate to the medium after phage infection. It is concluded that this effect depends upon the ability of ammonium sulphate to lower the rate of adsorption of phage upon host cells, thus preventing a high rate of phage loss by adsorption and inactivation towards the end of lysis. The possibility is discussed that this mechanism may explain some puzzling observations in lysing systems.


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