1887

Abstract

SUMMARY: Cohen & Wheeler medium prepared from casein hydrolysate previously treated with charcoal supported the growth of small inocula of (1–10 organisms/ml.).

Almost as good growth was obtained from small inocula in medium in which the casein hydrolysate was replaced by a balanced mixture of 17 amino acids and which contained a mixture of growth factors.

Medium containing either cystine and glutamic acid or cystine and aspartic acid as the only amino acids also supported the growth of small inocula; the rate of growth, however, was low.

Nicotinic acid proved to be an essential growth factor for all strains tested. Guanine, hypoxanthine and xanthine enhanced growth although they were not essential. Biotin and haemin, also not essential, enhanced growth during the early phase; this was especially evident with freshly isolated strains.

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1955-08-01
2022-01-16
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