1887

Abstract

Extracellular penicillinases produced by ATCC 9945A and from the same structural gene, , were compared. The two strains secreted the same exolarge penicillinase (mol. wt, 30500; isoelectric point, pI = 5.00-5.04; NH-terminal amino acid, Ser). In contrast, the exo-small enzyme from (mol. wt, 29500; pI = 5.00-5.04; NH-terminal amino acid, Glu or Asn) was slightly different from that of (mol. wt, 29500; pI = 5.13; NH-terminal amino acid, Lys). The difference in the NH-terminal residue is most probably due to differences in degradation by host-specific proteolytic enzymes.

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/content/journal/micro/10.1099/00221287-129-8-2621
1983-08-01
2021-10-23
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