1887

Abstract

Transient exposure of mycelia from to the cytidine analogue 5-azacytidine, at concentrations which do not affect the growth rate of the fungus on nearly minimal media, result in a dose-dependent, heritable change in the timing of the conidiation programme as well as heritable over-production of adaptive enzymes (glycosidases and phosphatases) and modification in the control properties of acid phosphatase. These heritable changes are induced by 5-azacytidine in a non-random way since the new phenotypes are exhibited not only by isolated clones but also by mixed populations of mycelia several life cycles (thousands of mitoses) after exposure to the drug.

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/content/journal/micro/10.1099/00221287-129-8-2585
1983-08-01
2021-05-06
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References

  1. Clutterbuck A. J. 1977; The genetics of conidia- tion in Aspergillus nidulans. In Genetics and Physiology of Aspergillus pp. 305–327 Smith J. E., Pateman J. A. Edited by London: Academic Press;
    [Google Scholar]
  2. Compere S. J., Palmiter R. D. 1981; DNA methylation controls the inducibility of the mouse metallothionein I gene in lymphoid cells. Cell 25:233–240
    [Google Scholar]
  3. Creusot F., Acs G., Christman J. K. 1982; Inhibition of DNA methyl transferase and induction of friend erythroleukemia cell differentiation by 5-azacytidine and 5-aza 2´-deoxycytidine. Journal of Biological Chemistry 257:2041–2048
    [Google Scholar]
  4. Ehrlich M., Wang R. Y. E. 1981; 5-Methylcystosine in eukaryotic DNA. Science 212:1350–1357
    [Google Scholar]
  5. Friedman S. 1979; The effect of 5-azacytidine on E. coli DNA-methylase. Biochemical and Biophysical Research Communications 89:1328–1333
    [Google Scholar]
  6. Groudine M., Eisenman R., Weintraub H. 1981; Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation. Nature, London 292:311–317
    [Google Scholar]
  7. Harris M. 1982; Induction of thymidine kinase in enzyme deficient Chinese hamster cells. Cell 29:483–492
    [Google Scholar]
  8. Hulme M. A., Stranks D. W. 1970; Induction and the regulation of production of cellulase by fungi. Nature, London 226:469–470
    [Google Scholar]
  9. Jinks J. L. 1966; Extranuclear inheritance. In The Fungi: An Advanced Treatise 2: pp. 619–660 Ainsworth G. C., Sussman A. S. Edited by New York: Academic Press;
    [Google Scholar]
  10. Jones P. A., Taylor S. M. 1980; Cellular differentiation, cytidine analogues and DNA methylation. Cell 20:85–93
    [Google Scholar]
  11. Jones P. A., Taylor S. M., Mohandas T., Shapiro L. J. 1982; Cell cycle-specific reactivation of an inactive X-chromosome locus by 5-azadeoxy-cytidine. Proceedings of the National Academy of Sciences of the United States of America 79:1215–1219
    [Google Scholar]
  12. Law D. J., Timberlake W. E. 1980; Developmental regulation of laccase levels in Aspergillus nidulans. Journal of Bacteriology 144:509–517
    [Google Scholar]
  13. Lemke P. A. 1981; Viruses of conidial fungi. In Biology of Conidial Fungi 2 pp. 395–416 Cole G. T., Kendrick B. Edited by New York: Academic Press;
    [Google Scholar]
  14. Niwa O., Sugahara T. 1981; 5-Azacytidine induction of mouse endogenous type C virus and suppression of DNA-methylation. Proceedings of the National Academy of Science of the United States of America 78:6290–6294
    [Google Scholar]
  15. Razin A., Riggs A. D. 1980; DNA methylation and gene function. Science 210:604–610
    [Google Scholar]
  16. Sager R., Kovac P. 1982; Pre-adipocyte determination either by insulin or by 5-azacytidine. Proceedings of the National Academy of Sciences of the United States of America 79:480–484
    [Google Scholar]
  17. Sakurai Y., Shiota H. 1980; Some properties of multiple acid phosphatases produced on phosphate restricted or enriched culture of Aspergillusoryzae. Journal of General and Applied Microbiology 26:315–329
    [Google Scholar]
  18. Smith J. E., Anderson J. G., Deans S. G., Davis B. 1977; Asexual development in Aspergillus. In Genetics and Physiology of Aspergillus pp. 23–58 Edited by Smith J. E., Pateman J. A. London: Academic Press;
    [Google Scholar]
  19. Taylor S. M., Jones P. A. 1979; Multiple new phenotypes induced in 10 T1/2 and 3T3 cells with 5-azacytidine. Cell 17:771–779
    [Google Scholar]
  20. Timberlake W. E. 1980; Developmental gene regulation in Aspergillus nidulans. Developmental Biology 78:497–510
    [Google Scholar]
  21. Timberlake W. E., Barnard E. C. 1981; Organization of a gene cluster expressed specifically in the asexual spores of A. nidulans. Cell 26:29–37
    [Google Scholar]
  22. Venolia L., Cartler S. M., Wassman E. R., Yen P., Mohandas T., Shapiro L. J. 1982; Transformation with DNA from 5-azacytidine-reactivated X-Chromosomes. Proceedings of National Academy of Sciences of the United States of America 79:2352–2354
    [Google Scholar]
  23. Zimmermann C. R., Orr W. C., Leclerck R. F., Barnard E. C., Timberlake W. E. 1980; Molecular cloning and selection of genes regulated in Aspergillus development. Cell 21:709–715
    [Google Scholar]
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