1887

Abstract

Batch cultures of X mut 1 lacking methanol dehydrogenase activity were grown in media containing both methylamine and ethanol as carbon source. The pattern of growth did not show the characteristics of diauxic growth but methylamine was the preferred substrate. When the concentration of the methylamine in the medium fell to 9 m (±0·8 m), ethanol was utilized and the two substrates were then utilized simultaneously until the methylamine was exhausted from the medium. Growth then continued using ethanol. The levels of key enzymes and the values for the two substrates supported this growth pattern and accounted for the measured uptake of the two substrates from the medium. When the wild-type X was used in similar experiments, methylamine and ethanol appeared to be used simultaneously. However, the values for methanol and ethanol were the same until ethanol dehydrogenase activity was induced. This suggested that the ethanol utilization observed before the induction of ethanol dehydrogenase was due to the activity of methanol dehydrogenase induced by the presence of methylamine. When this was taken into account the growth pattern obtained with the wild-type organism was the same as that seen with the mutant.

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/content/journal/micro/10.1099/00221287-129-8-2399
1983-08-01
2022-01-29
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References

  1. Attwood M.M., Harder W. 1974; The oxidation and assimilation of C2 compounds by Hyphomicrobium sp.. Journal of General Microbiology 84:350–356
    [Google Scholar]
  2. Attwood M.M., Harder W. 1977; Isocitrate lyase activity in Hyphomicrobium spp. - a critical reappraisal.. FEMS Microbiology Letters 1:25–30
    [Google Scholar]
  3. Attwood M.M., Harder W. 1978; Formate assimilation by Hyphomicrobium X.. FEMS Microbiology Letters 3:111–114
    [Google Scholar]
  4. Blackmore M.A., Quayle J.R. 1970; Microbial growth on oxalate by a route not involving glyoxylatecarboligase.. Biochemical Journal 118:53–59
    [Google Scholar]
  5. Dawson R.M.C., Elliott D.C., Elliott W.H., Jones K.M. 1969 Data for Biochemical Research, 2nd Edn. Oxford: Clarendon Press;
    [Google Scholar]
  6. Dijkhuizen L., Harder W. 1975; Substrate inhibition in Pseudomonas oxalaticus0X1: a kinetic study of growth inhibition by oxalate and formate using extended cultures.. Antonie van Leeuwenhoek 41:135–146
    [Google Scholar]
  7. Harder W., Attwood M.M. 1975; Oxidation of organic C, compounds by Hyphomicrobium spp.. Antonie van Leeuwenhoek 41:421–429
    [Google Scholar]
  8. Harder W., Attwood M.M., Quayle J.R. 1973; Methanol assimilation by Hyphomicrobiumsp.. Journal of General Microbiology 78:155–163
    [Google Scholar]
  9. Johnson P.A., Quayle J.R. 1964; Microbial growth on C, compounds. 6. Oxidation of methanol, formaldehyde and formate by methanol-grown Pseudomonas AMI.. Biochemical Journal 93:281–290
    [Google Scholar]
  10. Levering P.R., Van Dijken J.P., Veenhuis M., Harder W. 1981; Arthrobacter PI, a fast-growing versatile methylotroph with amine oxidase as a key enzyme in the metabolism of methylated amines.. Archives of Microbiology 129:72–80
    [Google Scholar]
  11. Marison I.W., Attwood M.M. 1982; A possible alternative mechanism for the oxidation of formaldehyde to formate.. Journal of General Microbiology 128:1441–1446
    [Google Scholar]
  12. Saier M.H.J., Roseman S. 1976; Sugar transport. Inducer exclusion and regulation of the melibiose, maltose, glycerol and lactate transport systems by the phosphoenolpyruvate: sugar phosphotransferase system.. Journal of Biological Chemistry 251:6606–6615
    [Google Scholar]
  13. Srere P.A. 1969; Citrate Synthase.. Methods in Enzymology 13:3–11
    [Google Scholar]
  14. Van Dijken J.P., Otto R., Harder W. 1975; Oxidation of methanol, formaldehyde and formate by catalase purified from methanol-grownHansen- ulapolymorpha.. Archives of Microbiology 106:221–226
    [Google Scholar]
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