Skip to content
1887

Microbiology Society logo Microbiology

Volume 129, Issue 7

Preliminary Identification of a Possible Cell-division Protein in the Cyanobacterium Free

Abstract

Cultures of the cyanobacterium Anacystis nidulans were grown under conditions of Mg-limitation and Mg-excess. Cell-free extracts, obtained after sonication and centrifugation (45000 , 60 min), were analysed on polyacrylamide gels. Mg-limited cells, in which cell division was inhibited, accumulated a protein of molecular weight 50 10. Only small amounts of this protein were detected in non-Mg-lirnited cultures. A protein of molecular weight 36 10, found in non-Mg-limited cells, was not detected in Mg-limited cells. When a Mg shift-up from 5 m to 1 mM was carried out in a chemostat, synthesis of the 36 103 protein was initiated and the amount of the 50 10 protein decreased whilst the main protein pattern remained unaltered. The possibility that the two proteins are involved in cell division is discussed.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology, 1983
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-7-2285
1983-07-01
2025-03-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/7/mic-129-7-2285.html?itemId=/content/journal/micro/10.1099/00221287-129-7-2285&mimeType=html&fmt=ahah

References

  1. Ahluwalia B., Duffus J. H., Paterson L. J., Walker G. M. 1978; Synchronization of cell division in the fission yeast Schizosaccharomyces pombe by ethylenediaminetetra-acetic acid. Journal of General Microbiology 106:261–264
     [Google Scholar]
  2. Ahmed N., Rowbury R. J. 1971; A temperature- sensitive cell division component in a mutant of Salmonella typhimurium. Journal of General Microbiology 67:107–115
     [Google Scholar]
  3. Grula E. A., King R. D. 1971; Changes in the cell membranes of dividing and non-dividing cells of Micrococcus lysodeikticusdis IIp+. Biochemical and Biophysical Research Communications 44:1356–1363
     [Google Scholar]
  4. Ingram L. O., Fisher W. D. 1973; Novel mutant impaired in cell division: evidence for a positive regulation factor. Journal of Bacteriology 113:999–1005
     [Google Scholar]
  5. Inouye M., Pardee A. B. 1970; Changes in membrane proteins and their relation to deoxyribonucleic acid synthesis and cell division of Escherichia coli. Journal of Biological Chemistry 245:5813–5819
     [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
     [Google Scholar]
  7. Lutkenhaus J. F., Donachie W. D. 1979; Identification of the ftsA gene product. Journal of Bacteriology 137:1088–1094
     [Google Scholar]
  8. Mann N., Carr N. G. 1977; Coupling between the initiation of DNA replication and cell division in the blue-green alga Anacystis nidulans. Archives of Microbiology 112:95–98
     [Google Scholar]
  9. Oakley B. R., Kirsch D. R., Morris N. R. 1980; A simplified ultrasensitive silver stain for detection of proteins in polyacrylamide gels. Analytical Biochemistry 105:361–363
     [Google Scholar]
  10. O’Farrell P. H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
     [Google Scholar]
  11. Petersen J. G. L., Kielland-Brandt M. C., Nilsson-Tillgren T. 1979; Protein patterns of yeast during sporulation. Carlsberg Research Communication 44:149–162
     [Google Scholar]
  12. Retovsky R., Klasterka I. 1961; Study of the growth and development of Chlorellapopulations in culture as a whole. V. The influence of the MgSO4 on autospore formation. Folia microbiologica 6:115–126
     [Google Scholar]
  13. Sachsenmaier W., Remy U., Plattner-Schobel R. 1972; Initiation of synchronous mitosis in Physarum polycephalum. Experimental Cell Research 73:41–48
     [Google Scholar]
  14. Smith H. S., Pardee A. B. 1970; Accumulation of a protein required for division during the cell cycle of Escherichia coli. Journal of Bacteriology 101:901–909
     [Google Scholar]
  15. Utkilen H. C. 1982; Magnesium-limited growth of the cyanobacteriumAnacystis nidulans. Journal of General Microbiology 128:1849–1862
     [Google Scholar]
  16. Webb M. 1949; The influence of magnesium on cell division. 2. The effect of magnesium on the growth and cell division of various bacterial species in complex media. Journal of General Microbiology 3:410–417
     [Google Scholar]
  17. Wheatley D. N., Rasmussen L., Zeuthen E. 1979; Analysis of the time of utilization of proteins for the initiation and completion of division in synchronized Tetrahymena pyriformis. Journal of Cell Science 37:109–116
     [Google Scholar]
/content/journal/micro/10.1099/00221287-129-7-2285
Loading
Preliminary Identification of a Possible Cell-division Protein in the Cyanobacterium Anacystis nidulans
Microbiology 129, 2285 (1983); https://doi.org/10.1099/00221287-129-7-2285
/content/journal/micro/10.1099/00221287-129-7-2285
/content/journal/micro/10.1099/00221287-129-7-2285
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error