1887

Abstract

Bacteriophage T4 wild-type is not sensitive to heating at 60 °C. Trypsin at this temperature quickly inactivates the bacteriophage, with first-order kinetics for about the first 60 min. The half-inactivation period is around 15 min. The characteristics of this inactivation reaction have been studied. Inactivation of T4 particles is paralleled by a loss in ability to adsorb to bacteria. SDS-PAGE reveals a ‘clipping’ of gp 37, the protein of the distal part of the long tail fibres, during the inactivation reaction, gp 37 is the only protein to be modified under these conditions. Mutants have been isolated which resist this modification; they map in gene 37.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-7-2217
1983-07-01
2021-07-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/7/mic-129-7-2217.html?itemId=/content/journal/micro/10.1099/00221287-129-7-2217&mimeType=html&fmt=ahah

References

  1. Adams M.H. 1959 Bacteriophages. New York: Wiley Interscience;
    [Google Scholar]
  2. Arscott P.G., Goldberg E.B. 1976; Cooperative action of the T4 tail fibers and base plate in triggering conformational change and in determining host range. Virology 69:15–22
    [Google Scholar]
  3. Beckendorf S.K. 1973; Structure of the distal half of the bacteriophage T4 tail fiber. Journal of Molecular Biology 73:37–53
    [Google Scholar]
  4. Beckendorf S.K., Kim J.S., Lielausis I. 1973; Structrure of bacteriophage T4 genes 37 and 38. Journal of Molecular Biology 73:17–35
    [Google Scholar]
  5. Bernstein H., Edgar R.S., Denhardt G.H. 1965; Intragenic complementation among temperature sensitive mutants of bacteriophage T4 D. Genetics 51:987–1002
    [Google Scholar]
  6. Cleveland D.W., Fisher S.G., Kirschner M.W., Laemmli U.K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulphate analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  7. Crawford J.T., Goldberg E.B. 1977; The effect of base plate mutants on the requirement for tail fiber binding for irreversible adsorption of bacteriophage T4. Journal of Molecular Biology 111:305–313
    [Google Scholar]
  8. Crawford J.T., Goldberg E.B. 1980; The function of tail fibers in triggering base plate expansion of bacteriophage T4. Journal of Molecular Biology 139:679–690
    [Google Scholar]
  9. Dawes J. 1975; Characterization of the bacteriophage receptor site. Nature, London 256:127–128
    [Google Scholar]
  10. Fisher K.M., Bernstein H. 1970; Deficiency of double mutant recombinants in crosses of phage T4. Molecular and General Genetics 106:139–150
    [Google Scholar]
  11. Goldberg A.L. 1972; Correlation between rates of degradation of bacterial proteins in vivo and their sensitivity to proteases. Proceedings of the National Academy of Sciences of the United States of America 69:2640–2644
    [Google Scholar]
  12. Goldberg A.L., Dice J.F. 1974; Intracellular protein degradation in mammalian cells: part 1. Annual Review of Biochemistry 43:835–869
    [Google Scholar]
  13. Goldberg A.L., St John A.C. 1976; Intracellular protein degradation in mammalian and bacterial cells: part 2. Annual Review of Biochemistry 45:747–803
    [Google Scholar]
  14. Kao S.H., Mcclain W.H. 1980; Base plate protein of bacteriophage T4 with both structural and lytic functions. Journal of Virology 34:95–103
    [Google Scholar]
  15. Kellenberger E., Bolle A., Boydelatour E., Epstein R.H., Franklin N.C., Jerne N.K., Reale-Scafati A., Sechaud J. 1965; Functions and properties related to the tail fibers of bacteriophage T4. Virology 26:419–440
    [Google Scholar]
  16. King J., Laemmli U.K. 1971; Polypeptides of the tail fibers of bacteriophage T4. Journal of Molecular Biology 62:465–477
    [Google Scholar]
  17. Oliver D.B., Crowther R.A. 1981; DNA sequence of the tail fiber gene 36 and 37 of bacteriophage T4. Journal of Molecular Biology 153:545–568
    [Google Scholar]
  18. Prehm P., Jann B., Jann K., Schmidt G., Stirm S. 1976; On a bacteriophage T3 and T4 receptor region within the cell wall lipopolysaccharide of Escherichia coli B. Journal of Molecular Biology 101:277–281
    [Google Scholar]
  19. Showe M.K., Isobe E., Onorato L. 1976; Bacteriophage T4 prehead proteinase. 1. Purification and properties of bacteriophage enzyme which cleaves the capsid precursor proteins. Journal of Molecular Biology 107:35–54
    [Google Scholar]
  20. Simon L.D., Anderson T.F. 1967; The infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope. 1. Attachment and penetration. Virology 32:279–297
    [Google Scholar]
  21. Simon L.D., Tomczak K., St John A.C. 1978; Bacteriophages inhibit degradation of abnormal proteins in E. coli. Nature, London 275:424–428
    [Google Scholar]
  22. Stahl F.W., Crasemann J.M., Yegian C., Stahl M.M., Nakata A. 1970; Co-transcribed cistrons in bacteriophage T4. Genetics 64:157–170
    [Google Scholar]
  23. Terzaghi E. 1971; Alternative pathways of tail fiber assembly in bacteriophage T4. Journal of Molecular Biology 59:319–327
    [Google Scholar]
  24. To C.M., Kellenberger E., Eisenstark A. 1969; Disassembly of T-even bacteriophage into structural parts and subunits. Journal of Molecular Biology 46:493–511
    [Google Scholar]
  25. Ward S., Dickson R.C. 1971; Assembly of bacteriophage T4 tail fibers. III. Genetic control of the major tail fiber polypeptides. Journal of Molecular Biology 62:479–492
    [Google Scholar]
  26. Wood W.B., Bishop R.J. 1973; Bacteriophage T4 tail fibers: structure and assembly of a viral organelle. In Virus Research pp. 303–324 Edited by Fox C. F., Robinson W. S. New York: Academic Press;
    [Google Scholar]
  27. Wood W.B., Henninger M. 1969; Attachment of tail fibers in bacteriophage T4 assembly: some properties of the reaction in vitro and its genetic control. Journal of Molecular Biology 39:603–618
    [Google Scholar]
  28. Wood W.B., Dickson R.C., Bishop R.J., Revel H.R. 1972; Self-assembly and non-self-assembly in bacteriophage T4 morphogenesis. In The Generation of Subcellular Structures pp. 25–59 Edited by Markham R., Bancroft J. B., Davies D. R., Hopwood D. A., Horne R. W. Amsterdam: North-Holland Publishing Company;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-7-2217
Loading
/content/journal/micro/10.1099/00221287-129-7-2217
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error