1887

Abstract

FeCI labelling and non-denaturing gel electrophoresis were used to study nitrogenase synthesis in sp. CCAP 1430/3. Nitrogenase synthesis was inhibited by addition of NHX but was unaffected by elevated concentrations of O. Upon transfer of cultures of from light to darkness, there was initially a slight decrease in the rate of synthesis of nitrogenase but after 4–5 h there was an almost complete cessation of synthesis. This delayed effect of darkness on nitrogenase synthesis could not be related to any change in RNA synthesis, in protein synthesis or in the rate of breakdown of storage glucan.

In cultures of mRNA, including mRNA, was unstable, having a half-life of about 5 min. The synthesis of mRNA did not stop immediately upon transfer of cultures to the dark. If darkness exerts its effect on nitrogenase synthesis by inhibiting the synthesis of mRNA, it does so only after a lag of about 4 h.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-6-1689
1983-06-01
2021-05-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/6/mic-129-6-1689.html?itemId=/content/journal/micro/10.1099/00221287-129-6-1689&mimeType=html&fmt=ahah

References

  1. Bailey J. L. 1962 Techniques in Protein Chemistry, 1st edn. p. 293 Amsterdam: Elsevier;
    [Google Scholar]
  2. Dingman C. W., Peacock A. C. 1968; Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis. Biochemistry 7:659–668
    [Google Scholar]
  3. Doolittle W. F. 1972; Ribosomal ribonucleic acid synthesis and maturation in the blue-green alga Anacystis nidulans. . Journal of Bacteriology 111:316–324
    [Google Scholar]
  4. Eady R. 1981; Regulation of nitrogenase activity. In Current Perspectives in Nitrogen Fixation pp. 172–181 Edited by Gibson A. H., Newton W. E. Canberra: Australian Academy of Science;
    [Google Scholar]
  5. Eady R. R., Issack R., Kennedy C., Postgate J. R. 1978; Nitrogenase synthesis in Klebsiella pneumoniae: Comparison of ammonium and oxygen regulation. Journal of General Microbiology 104:277–285
    [Google Scholar]
  6. Eady R., Kahn D., Hawkins M. 1981; Metabolic control of Klebsiella pneumoniae mRNA degradation: significance to nif regulation by NHJ. In Current Perspectives in Nitrogen Fixation p. 443 Edited by Gibson A. H., Newton W. E. Canberra: Australian Academy of Science;
    [Google Scholar]
  7. Gallon J. R. 1981; The oxygen sensitivity of nitrogenase: a problem for biochemists and microorganisms. Trends in Biochemical Sciences 6:19–23
    [Google Scholar]
  8. Gallon J. R., Kurz W. G. W., Larue T. A. 1973; Isocitrate supported nitrogenase activity in Gloeocapsa sp. LB795. Canadian Journal of Microbiology 19:461–465
    [Google Scholar]
  9. Hallenbeck P. C., Kostel P. J., Benemann J. R. 1979; Purification and properties of nitrogenase from the cyanobacterium, Anabaena cylindrica. . European Journal of Biochemistry 98:275–284
    [Google Scholar]
  10. Hamadi A. F., Gallon J. R. 1981; Calcium ions, oxygen and acetylene reduction (nitrogen fixation) in the unicellular cyanobacterium Gloeocapsa sp. 1430/3. Journal of General Microbiology 125:391–398
    [Google Scholar]
  11. Kirby K. S., Fox-Carter E., Guest M. 1967; Isolation of deoxyribonucleic acid and ribosomal ribonucleic acid from bacteria. Biochemical Journal 104:258–262
    [Google Scholar]
  12. Lowe D. J., Smith B. E., Eady R. R. 1980; The structure and mechanism of nitrogenase. In Recent Advances in Biological Nitrogen Fixation pp. 34–87 Edited by Subba N. Rao London: Edward Arnold;
    [Google Scholar]
  13. Meeks J. C., Wolk C. P., Thomas J., Lockau W., Shaffer P. W., Austin S. M., Chien W.-S., Galonsky A. 1977; The pathways of assimilation of 13NHJ by the cyanobacterium, Anabaena cylindrica. . Journal of Biological Chemistry 252:7894–7900
    [Google Scholar]
  14. Mullineaux P. M., Chaplin A. E., Gallon J. R. 1980; . Effects of a light to dark transition on carbon reserves, nitrogen fixation and ATP concentrations in cultures of Gloeocapsa(Gloeothece) sp. 1430/3. Journal of General Microbiology 120:227–232
    [Google Scholar]
  15. Mullineaux P. M., Gallon J. R., Chaplin A. E. 1981a; Acetylene reduction (nitrogen fixation) by cyanobacteria grown under alternating light-dark cycles. FEMS Microbiology Letters 10:245–247
    [Google Scholar]
  16. Mullineaux P. M., Gallon J. R., Chaplin A. E. 1981b; Nitrogen fixation in cultures of the cyanobacterium Gloeocapsa (Gloeothece) sp. 1430/3 incubated in the dark. Journal of General Microbiology 124:141–146
    [Google Scholar]
  17. Peterson R. B., Wolk C. P. 1978; High recovery of nitrogenase activity and of 55Fe-labelled nitrogen- ase in heterocysts isolated from Anabaena variabilis. . Proceedings of the National Academy of Sciences of the United States of America 75:6271–6275
    [Google Scholar]
  18. Robson R. L. 1979; O2-repression of nitrogenase synthesis in Azotobacter chroococcum. . FEMS Microbiology Letters 5:259–262
    [Google Scholar]
  19. Smith R. J., Carr N. G. 1977; An investigation of RNA synthesis in Anacystis nidulans during exponential growth using techniques of RNA-DNA hybridization. Journal of General Microbiology 98:559–567
    [Google Scholar]
  20. Solymosy F., Fedorcsák I., Galyás A., Farkas G. S., Ehrenberg L. 1968; A new method based on the use of diethyl pyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid from plant tissues. European Journal of Biochemistry 5:520–527
    [Google Scholar]
  21. Tözö S. R. D., Ul-Haque M. I., Chaplin A. E., Gallon J. R. 1977; The effect of fluoroacetate on acetylene reduction by Gloeocapsa. . Biochemical Society Transactions 5:1482–1484
    [Google Scholar]
  22. Tözö S. R. D., Gallon J. R. 1979; The effects of methyl viologen on Gloeocapsa sp. LB795 and their relationship to the inhibition of acetylene reduction (nitrogen fixation) by oxygen. Journal of General Microbiology 111:313–326
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-6-1689
Loading
/content/journal/micro/10.1099/00221287-129-6-1689
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error