1887

Abstract

The soluble cytochrome oxidase of has been highly purified and shown to be a copper protein devoid of haem, not a cytochrome as was previously assumed. The native molecular weight was 120000 and the subunit molecular weight 35000. Soluble cytochrome oxidase activity co-purified with nitrite reductase activity; both activities were almost certainly associated with the same protein. The possible physiological role of the nitrite reductase activity is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-6-1645
1983-06-01
2021-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/6/mic-129-6-1645.html?itemId=/content/journal/micro/10.1099/00221287-129-6-1645&mimeType=html&fmt=ahah

References

  1. Anderson J. R., Strumeyer D. H., Pramer D. 1968; Purification and properties of peroxidase from Nitrosomonas europaea. . Journal of Bacteriology 96:93–97
    [Google Scholar]
  2. Erickson R. H., Hooper A. B. 1968; A soluble hydroquinone oxidase from Nitrosomonas europaea. . Bacteriological Proceedings 68:140
    [Google Scholar]
  3. Erickson R. H., Hooper A. B., Terry K. R. 1972; Solubilization and purification of cytochrome a1 from Nitrosomonas. . Biochimica et biophysica acta 283:155–166
    [Google Scholar]
  4. Hooper A. B. 1968; A nitrite reducing enzyme from Nitrosomonas europaea. Preliminary characterization with hydroxylamine as electron donor. Biochimica et biophysica acta 162:49–65
    [Google Scholar]
  5. Hooper A. B., Erickson R. H., Terry K. R. 1972; Electron transport systems in Nitrosomonas:isolation of a membrane-envelope fraction. Journal of Bacteriology 110:430–438
    [Google Scholar]
  6. Hooper A. B., Terry K. R., Maxwell P. C. 1977; Hydroxylamine oxidoreductase of Nitro- somonas: oxidation of diethyldithiocarbamate concomitant with stimulation of nitrite synthesis. Bio-chimica et biophysica acta 462:141–152
    [Google Scholar]
  7. Ingledew W. J. 1978; Cytochrome-a1 as an oxidase?. In Functions of Alternative Terminal Oxidases Proceedings of the 11th FEBS Meeting pp. 79–87 Degn H., Lloyd D., Hill G. C. Edited by Oxford: Pergamon Press;
    [Google Scholar]
  8. Iwasaki H., Matsubara T. 1972; A nitrite reductase from Achromobacter cycloclastes. . Journal of Biochemistry 71:645–652
    [Google Scholar]
  9. Iwasaki H., Noji S., Shidara S. 1975; Achromobacter cycloclastes nitrite reductase. The function of copper, amino acid composition and ESR spectra. Journal of Biochemistry 78:355–361
    [Google Scholar]
  10. Iwasaki H., Shidara S., Suzuki H., Mori T. 1963; Studies on denitrification. VII. Further purification and properties of denitrifying enzyme. Journal of Biochemistry 53:299–303
    [Google Scholar]
  11. Kakutani T., Watanabe H., Arima K., Beppu T. 1981; Purification and properties of a copper- containing nitrite reductase from a denitrifying bacterium, Alcaligenes faecalis strain S-6. Journal of Biochemistry 89:453–461
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  13. Lipschultz F., Zafiriou O. C., Wofsy S. C., Mcelroy M. B., Valois F. W., Watson S. W. 1981; Production of NO and NiObysoil nitrifying bacteria. Nature; London: 294641–643
    [Google Scholar]
  14. Mejbaum-Katzenellenbogen W. 1955; A new turbidometric micromethod for protein analysis. Bulletin de l’Academie polonaise des sciences, Classe II 3:171–173
    [Google Scholar]
  15. Miller D. J., Wood P. M. 1982; Characterization of the c-type cytochromes of Nitrosomonas europaea with the aid of fluorescent gels. Biochemical Journal 207:511–517
    [Google Scholar]
  16. Miyata M., Mori T. 1968; Studies on denitrification. VIII. Production of nitric oxide by denitrifying reaction in the presence of tetramethyl-p-phenylene- diamine. Journal of Biochemistry 64:849–861
    [Google Scholar]
  17. Miyata M., Mori T. 1969; Studies on denitrification. X. The denitrifying enzyme as a nitrite reductase and the electron donating system for denitrification. Journal of Biochemistry 66:463–471
    [Google Scholar]
  18. Rees M. K. 1968; Studies of the hydroxylamine metabolism of Nitrosomonas. I. Purification of hydroxylamine oxidase. Biochemistry 7:353–365
    [Google Scholar]
  19. Rees M. K., Nason A. 1965; A P-450-like cytochrome and a soluble terminal oxidase identified as cytochrome-o from Nitrosomonas europaea. . Biochemical and Biophysical Research Communications 21:248–256
    [Google Scholar]
  20. Ritchie G. A. F., Nicholas D. J. D. 1972; Identification of the sources of nitrous oxide produced by oxidative and reductive processes in Nitrosomonas europaea. . Biochemical Journal 126:1181–1191
    [Google Scholar]
  21. Ritchie G. A. F., Nicholas D. J. D. 1974; The partial characterization of purified nitrite reductase and hydroxylamine oxidase from Nitrosomonas europaea. . Biochemical Journal 138:471–480
    [Google Scholar]
  22. Sawada E., Satoh T. 1980; Periplasmic location of dissimilatory nitrate and nitrite reductase in a denitrifying phototrophic bacterium Rhodopseudo- monas sphaeroides forma sp. denitrificans. . Plant and Cell Physiology 21:205–210
    [Google Scholar]
  23. Sawada E., Satoh T., Kitamura H. 1978; Purification and properties of a dissimilatory nitrite reductase of a denitrifying phototrophic bacterium. Plant and Cell Physiology 19:1339–1351
    [Google Scholar]
  24. Warburg O., Christian W. 1941; Isolierung und Kristallisation des gärungsferments Enolase. Bio-chemische Zeitschrift 310:384–421
    [Google Scholar]
  25. Weston J. A., Knowles C. J. 1973; A soluble CObinding c-type cytochrome from Beneckea natrie-gens. . Biochimica et biophysica acta 305:11–18
    [Google Scholar]
  26. Wood P. M. 1978; Periplasmic location of the terminal reductase in nitrite respiration. FEBS Letters 92:214–218
    [Google Scholar]
  27. Yamanaka T., Okunuki K. 1963; Crystalline Pseudomonas cytochrome oxidase. I. Enzymic properties with special reference to the biological specificity. Biochimica et biophysica acta 67:379–393
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-6-1645
Loading
/content/journal/micro/10.1099/00221287-129-6-1645
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error